1kb DNA Ladder
Kufotokozera Kwa Zida:
◮Makwerero a DNA a 1Kb amapangidwa ndi DNA ya 13 iwiri, ndipo kuwirikiza kawiri kwa 1kb ndi 5kb kumathandiza kusiyanitsa magulu osiyanasiyana.Pansi pa 1kb, magulu atatu a 250bp, 500bp, ndi 750bp amawonjezeredwa, ndipo kukula kwachidutswa chachifupi kungathe kuwerengedwa molingana ndi izi.Kuchuluka kwa magulu anayi a 6kb, 7kb, 8kb, ndi 10kb ndi theka, zomwe zimapindulitsa kupeza zotsatira zolekanitsa bwino.
Kufotokozera
Makwerero a DNA a 1Kb amapangidwa ndi DNA ya 13 iwiri, ndipo kuwirikiza kawiri kwa 1kb ndi 5kb kumathandiza kusiyanitsa magulu osiyanasiyana.Pansi pa 1kb, magulu atatu a 250bp, 500bp, ndi 750bp amawonjezeredwa, ndipo kukula kwachidutswa chachifupi kungathe kuwerengedwa molingana ndi izi.Kuchuluka kwa magulu anayi a 6kb, 7kb, 8kb, ndi 10kb ndi theka, zomwe zimapindulitsa kupeza zotsatira zolekanitsa bwino.
Izi zili kale ndi 1× Loading Buffer.Mukhoza kutenga 2-6μl kwa electrophoresis mwachindunji malinga ndi zosowa za kuyesa.Chithunzi cha electrophoresis ndi chomveka, chothandiza komanso chothandiza.
Magulu 13 a mankhwalawa kuyambira ang'onoang'ono mpaka akulu ndi awa: 250bp, 500bp, 750bp, 1kb, 1.5kb, 2kb, 3kb, 4kb, 5kb, 6kb, 7kb, 8kb, 10kb.Ngati kukweza voliyumu ndi 5μl, 1kb ndi 5kb magulu ndi 100ng;6kb, 7kb, 8kb, ndi 10kb magulu ndi 25ng;ndipo magulu otsala ndi 50ng.
Zofotokozera
| Mtengo wa DL-30011 | Kukonzekera 50 (250μl) |
| Mtengo wa DL-30012 | 200 Preps (1 ml) |
Zida zamagulu
Zosungirako zamadzimadzi
10mM Tris-HCl(pH 8.4) 10mM EDTA
0.02% BPB
5% glycerin
Mankhwala ndende
Kuchuluka kwa mankhwalawa ndi pafupifupi 130μg/ml
Electropherogram
Zosungirako
Chonde sungani pa 4℃(chonde sungani pa -20℃kusungirako nthawi yayitali)
