SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Njira)

Kufotokozera Kwa Zida:

Chidachi chimagwiritsa ntchito ukadaulo wa Real-time RT PCR(rRT-PCR) pozindikira bwino za SARS-CoV-2 (ORF1ab gene ndi N gene) ma nucleic acid mu zitsanzo za nasopharyngeal kapena oropharyngeal swab za anthu, pogwiritsa ntchito njira yofufuzira ya multiplex PCR fulorosenti. Kuwongolera kwamkati kuyesa mtundu wa zitsanzo, kotero kuti palibe chifukwa choyeretsera ma nucleic acid, kumatha kuyesedwa mu ola limodzi, makamaka koyenera kuzindikira mwachangu.

foregene strength


Tsatanetsatane wa Zamalonda

Zogulitsa Tags

Zofotokozera

48 Rxns, 96 Rxns

Ayi.

Chigawo

Ndalama

Zigawo zazikulu

48 nsi

96 nsi

1 Nucleic acid kumasula wothandizira 1.4mL / chubu 2 machubu 5.3 mL / botolo 1 botolo Wokwera pamwamba
2 Chitetezo cha RNA 27 μL / chubu 1 chubu 53 μL / chubu 1 chubu RNase inhibitor
3 SARS-CoV-2 reaction solution 800 μL / chubu 1 chubu 1600 μL / chubu 1 chubu Poyamba, probe, reaction buffer, dNTP
4 SARS-CoV-2 enzyme osakaniza 80 μL / chubu 1 chubu 160 μL / chubu 1 chubu Kutentha koyambira kwa Taq enzyme, enzyme ya M-MLV
5 Kuwongolera kwabwino kwa SARS-CoV-2 100 μL / chubu 1 chubu 100 μL / chubu 1 chubu Recombinant plasmid yokhala ndi chidutswa chandamale, RNA
6 Kuwongolera koyipa kwa SARS-CoV-2 1200 μL / chubu 1 chubu 1200 μL / chubu 1 chubu TE buffer

Mbali&zabwino

Mwamsanga ndi zosavuta.
Pewani kutaya kwa nucleic acid.
Malire otsika ozindikira komanso kukhudzidwa kwakukulu.
Kufunika kwa zida zochepa komanso kugwiritsa ntchito kosavuta.

Kuzindikira kumatha kufika pamlingo wotsika kwambiri ngati makope 500/mL).

GCE/mL Mtengo wapatali wa magawo Ct
N gene Mtengo wa ORF1ab
2.00E + 08 16.43 15.16
2.00E + 07 19.48 18.58
2.00E + 06 22.71 21.86
2.00E + 05 25.95 25.33
2.00E + 04 29.30 28.49
2.00E + 03 32.37 31.37
2.00E + 02 35.32 34.47
2.00E + 01 38.29 38.27
2.00E + 00 N / A N / A
2.00E-01 N / A N / A
NC N / A N / A

Linear amplification curve

amplification curve-RT-qPCR

Momwe mungagwiritsire ntchito

1. Chida chothandizira

covid-19 nucleic acid detection kit1

2. Njira yodziwira

covid-19 nucleic acid detection kit2

Kukonzekera kwachitsanzo: Naopharyngeal kapena oropharyngeal swab chitsanzo (ntchito imodzi yokha) (Dikirani kwa mphindi 10)

Kukulitsa kwa PCR (<=55min)

Kayendedwe kantchito:

covid-19 nucleic acid detection kit4

Kusungirako

osindikizidwa kuchokera kuwala ndi kusungidwa pa -20 ± 5 ℃;

storage
storage2

Alumali moyo: 1 chaka


  • Zam'mbuyo:
  • Ena:

  • Lembani uthenga wanu apa ndikutumiza kwa ife