China SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method) Wopanga ndi Wopereka |Foregene

SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Njira)

SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method) Chithunzi Chowonetsedwa

Kufotokozera Kwa Zida:

◮ Direct PCR:Palibe chifukwa cha zida za nucleic acid extaction ndi System, zomaliza 96 mu ola limodzi kapena apo.

◮ Kufunika kwa zida zochepa komanso kugwiritsa ntchito kosavuta:Kungofunika Real-Time PCR system .

◮ LoD komanso kukhudzika kwakukulu:Kuzindikira kumatha kufika pamlingo wotsika kwambiri ngati makope 500/ml.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Cholinga Chogwiritsidwa Ntchito

Chidachi chimagwiritsa ntchito ukadaulo wa Real-time RT PCR (rRT-PCR) pozindikira zamtundu wa SARS-CoV-2 nucleic acid mu zitsanzo za nasopharyngeal kapena oropharyngeal swab.

Mfundo yoyesera

Zoyambira ndi zoyesererazi zidapangidwa kuti zizindikire zotsatizana zosungidwa za SARS-CoV-2 (ORF1ab gene ndi N gene).Chidachi chili ndi Internal Control yomwe imagwiritsidwa ntchito kuwunika mtundu wa zitsanzo.

Tekinoloje ya Real-Time PCR imagwiritsa ntchito polymerase chain reaction (PCR) kukulitsa chandamale.

kutsata ndi kutsata zofufuza zenizeni zowunikira RNA yokwezeka.Ma probe amalembedwa ndi

mtolankhani wa fulorosenti ndi utoto wozimitsa.

Zambiri Zachangu

◮Zofotokozera:48 Rxns/kit, 96 Rxns/kit

◮ Mwatsatanetsatane:96.72%

Kukhudzika:97.92%

◮ Mitundu ya zitsanzo zovomerezeka

Nasopharyngeal Swab kapena Oropharyngeal Swab

◮Chida chogwiritsidwa ntchito

ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S

Zigawo

Ayi.	Chigawo	Ndalama				Zigawo zazikulu
Ayi.	Chigawo	48 nsi		96 nsi		Zigawo zazikulu
1	Nucleic acid kumasula wothandizira	1.4mL / chubu	2 machubu	5.3 mL / botolo	1 botolo	Wokwera pamwamba
2	Chitetezo cha RNA	27 μL / chubu	1 chubu	53 μL / chubu	1 chubu	RNase inhibitor
3	SARS-CoV-2 reaction solution	800 μL / chubu	1 chubu	1600 μL / chubu	1 chubu	Poyamba, probe, reaction buffer, dNTP
4	SARS-CoV-2 enzyme osakaniza	80 μL / chubu	1 chubu	160 μL / chubu	1 chubu	Kutentha koyambira kwa Taq enzyme, enzyme ya M-MLV
5	Kuwongolera kwabwino kwa SARS-CoV-2	100 μL / chubu	1 chubu	100 μL / chubu	1 chubu	Recombinant plasmid yokhala ndi chidutswa chandamale, RNA
6	Kuwongolera koyipa kwa SARS-CoV-2	1200 μL / chubu	1 chubu	1200 μL / chubu	1 chubu	TE buffer