Animal miRNA Isolation Kit MicroRNA Extraction and Purification Kits for Animal
Kufotokozera Kwa Zida:
Mwachangu komanso mogwira mtima timachotsa zidutswa zing'onozing'ono za RNA za 20-200nt miRNA, siRNA, snRNA kuchokera kumagulu osiyanasiyana anyama ndi ma cell.
◆Njira yonseyi imayendetsedwa pa kutentha kwapakati (15-25 ° C), popanda madzi oundana komanso kutentha pang'ono centrifugation.
◆Zida zonse za RNase-Free, palibe chifukwa chodera nkhawa za kuwonongeka kwa RNA.
◆DNA-Cleaning Column imamangiriza DNA, kuti zida zitha kuchotsa kuipitsidwa kwa DNA popanda kuwonjezera DNase.
◆Zokolola zapamwamba za RNA: Mzere wa RNA-okha ndi fomula yapadera imatha kuyeretsa RNA bwino.
◆Kuthamanga Kwambiri: Ntchitoyi ndi yosavuta ndipo imatha kutha mkati mwa mphindi 30.
◆Chitetezo: palibe organic reagent m'zigawo zofunika.
◆Ubwino wapamwamba: Zidutswa za RNA zochotsedwa ndizoyera kwambiri, zopanda mapuloteni ndi zonyansa zina, ndipo zimatha kukwaniritsa zofunikira zakutsikira kwamtsinje.
◆Zoyeserera zosiyanasiyana ntchito
Kufotokozera
Chidachi chimagwiritsa ntchito spin column ndi formula yopangidwa ndi kampani yathu, yomwe imatha kuchotsa tiziduswa tating'ono ta RNA monga 20-200nt miRNA, siRNA, snRNA kuchokera kumagulu osiyanasiyana anyama ndi ma cell.Chidachi chimapereka DNA-Cleaning Column, yomwe imatha kupatutsa chodabwitsa kwambiri kuchokera ku minofu ya lysate ndikuchotsa DNA ndi RNA yayitali kuchokera pachitsanzo, chomwe ndi chosavuta komanso chopulumutsa nthawi;Mzere wa RNA wokha ukhoza kumangirira RNA ndi chilinganizo chapadera , Ingathe kukonza zitsanzo zambiri nthawi imodzi.Dongosolo lonseli ndi RNase-Free, kotero kuti miRNA yoyeretsedwa isawonongeke;Buffer miRW1, Buffer miRW2 buffer system yotsuka, kuti RNA yopezedwayo ilibe mapuloteni, DNA, ayoni, ndi kuipitsidwa kwapawiri.
Zida zamagulu
| Animal miRNA Kudzipatula Kit | ||||
| Zamkatimu | RE-0101T | RE-01011 | RE-01012 | RE-01014 |
| 5 T | 50 T | 100T | 200T | |
| Buffer miRL* | 3ml ku | 25ml ku | 50 ml pa | 100 ml |
| Mtengo wa miRW1 | 4ml ku | 35ml ku | 70 ml pa | 140 ml |
| Mtengo wa miRW2 | 2.4ml pa | 24ml ku | 48ml ku | 96ml pa |
| RNase-Free ddH2O | 1.7ml pa | 10 ml pa | 20 ml pa | 40 ml pa |
| Mzere wa RNA wokha | 5 seti | 50seti | 100seti | 200seti |
| DNA-Kuyeretsa Mzere | 5 seti | 50seti | 100seti | 200seti |
| IFU | 1 chidutswa | 1 chidutswa | 1 chidutswa | 1 chidutswa |
Mbali & ubwino
◆Njira yonseyi imayendetsedwa pa kutentha kwapakati (15-25 ° C), popanda madzi oundana komanso kutentha pang'ono centrifugation.
◆Zida zonse za RNase-Free, palibe chifukwa chodera nkhawa za kuwonongeka kwa RNA.
◆DNA-Cleaning Column imamangiriza DNA, kuti zida zitha kuchotsa kuipitsidwa kwa DNA popanda kuwonjezera DNase.
◆Zokolola zapamwamba za RNA: Mzere wa RNA-okha ndi fomula yapadera imatha kuyeretsa RNA bwino.
◆Kuthamanga Kwambiri: Ntchitoyi ndi yosavuta ndipo imatha kutha mkati mwa mphindi 30.
◆Chitetezo: palibe organic reagent m'zigawo zofunika.
◆Ubwino wapamwamba: Zidutswa za RNA zochotsedwa ndizoyera kwambiri, zopanda mapuloteni ndi zonyansa zina, ndipo zimatha kukwaniritsa zofunikira zakutsikira kwamtsinje.
◆Zoyeserera zosiyanasiyana ntchito
Kit application
Ndiwoyenera kuchotsedwa ndi kuyeretsedwa kwa miRNA kuchokera kumitundu yosiyanasiyana yanyama yatsopano kapena yozizira kapena ma cell.
Kusungirako ndi moyo wa alumali
Chidachi chikhoza kusungidwa kwa miyezi 24 pansi pouma kutentha kwa firiji (15-25 ° C), ngati ikufunika kusungidwa kwa nthawi yayitali, ikhoza kusungidwa2-8 ° C.Buffer miRL ikhoza kuyikidwa pa4°Ckwa mwezi 1 mutawonjezeraβ-mercaptoethanol(ndikofunikira kuti muwonjezere tsopano).
