Bakiteriya DNA Isolation Kit Bakiteriya Genomic DNA M'zigawo Kuyeretsa Kits
Kufotokozera Kwa Zida:
Yeretsani mwachangu ma genomic DNA kuchokera ku mabakiteriya omwe ali mu gawo la kukula kwa logarithmic.
◮Palibe kuipitsidwa kwa RNase:DNA-Only Column yoperekedwa ndi zidayi imapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda kuwonjezera RNase panthawi yoyesera, kuletsa labotale kuti isaipitsidwe ndi RNase yachilendo.
◮Liwiro:Foregene Protease imakhala ndi zochita zambiri kuposa ma protease ofanana, ndipo imagaya zitsanzo za minofu mwachangu;ntchitoyo ndi yosavuta, ndipo ntchito yochotsa DNA ya genomic imatha kutha mkati mwa mphindi 20-80.
◮Zabwino:The centrifugation ikuchitika kutentha firiji, ndipo palibe chifukwa 4 ° C otsika kutentha centrifugation kapena Mowa mpweya wa DNA.
◮Chitetezo:Palibe organic reagent m'zigawo zofunika.
◮Mapangidwe apamwamba:DNA yotulutsidwa ya genomic ili ndi zidutswa zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira zosiyanasiyana.
◮Microelution System:Itha kuwonjezera kuchuluka kwa ma DNA amtundu, omwe ndi osavuta kuzindikira kapena kuyesa kuyesa.
Kufotokozera
Chidachi chimapereka njira yofulumira komanso yosavuta yochotsera DNA ya genomic ku zikhalidwe za mabakiteriya (Gram-negative ndi Gram-positive) kuchokera kuzinthu zosiyanasiyana;imatha kukonza zosakwana 3ml za chikhalidwe cha mabakiteriya mu gawo la kukula kwa logarithmic (1 × 109 mabakiteriya).Chidacho chikufanana ndi Foregene Protease yapamwamba kwambiri, yomwe imalola 15-50μg ya DNA yapamwamba kwambiri ya genomic kuti ichotsedwe mkati mwa ola limodzi.Kuphatikiza apo, zida zimathanso kutulutsa ma genetic kupatula ma genome, monga plasmids, Cosmid, BAC, ndi zina.
DNA-yokha silica gel matrix zakuthupi zomwe zimagwiritsidwa ntchito pozungulira ndi chinthu chatsopano cha kampani, chomwe chimatha kutsatsa DNA moyenera komanso mwapadera.Kuchuluka kwa adsorption kwa DNA ndi 80μg.Dongosolo lapadera la buffer ndi elution limatha kukulitsa kuchotsedwa kwa RNA, mapuloteni Osadetsedwa, ayoni ndi ma organic compounds m'maselo.Chidutswa cha DNA chotengedwa ndi chachikulu, choyera kwambiri, chokhazikika komanso chodalirika, ndipo kukula kwa chidutswa cha DNA ndi chokhazikika pafupifupi 23kb.
Zofotokozera
50 Preps, 100 Preps, 250 Preps
Zida zamagulu
| Mtengo wa ML1 |
| Mtengo wa ML2 |
| Mtengo PW |
| Mtengo WB |
| Mtengo wa EB |
| Foregene Protease |
| Lysozyme |
| Mtengo wa TE |
| Nambala ya DNA Yokha |
| Malangizo |
Mbali & ubwino
-Palibe kuipitsidwa kwa RNase: Palibe chifukwa chowonjezera RNase kuti muchotse RNA mu genomic DNA, kupewa labotale kuti isaipitsidwe ndi RNase yakunja.
-Kuthamanga Kwambiri: Foregene Protease imakhala ndi zochita zambiri kuposa ma protease ofanana ndipo imatha kugaya zitsanzo za mabakiteriya osweka pakanthawi kochepa.
-Kuthandiza: The centrifugation ikuchitika kutentha firiji, ndipo palibe chifukwa 4 ° C otsika kutentha centrifugation kapena Mowa mpweya wa DNA.
- Chitetezo: palibe organic reagent m'zigawo zofunika.
-Zapamwamba: Zidutswa za DNA zomwe zatulutsidwa ndi zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira pazoyeserera zosiyanasiyana.
Kit application
Chidachi ndi choyenera kuyeretsa DNA ya ma genomic mwa zitsanzo zotsatirazi: mabakiteriya a gram-negative ndi mabakiteriya a gram-positive mu gawo la kukula kwa logarithmic.
Kayendedwe kantchito
Kusungirako ndi moyo wa alumali
-Chida ichi chikhoza kusungidwa kwa miyezi 12 pansi pa nyengo youma kutentha (15-25 ° C);Ngati ikufunika kusungidwa kwa nthawi yayitali, imatha kusungidwa pa 2-8 ° C.
Zindikirani: Ngati atasungidwa kutentha kochepa, yankho limakhala lamvula.Musanagwiritse ntchito, onetsetsani kuti mwayika yankholo mu zida pa kutentha kwapakati kwa nthawi.Ngati n'koyenera, preheat mu madzi osamba 37 ° C kwa mphindi 10 kuti asungunuke mpweya, ndikusakaniza musanagwiritse ntchito.
-Foregene Protease yankho lili ndi ndondomeko yapadera, yomwe imagwira ntchito ikasungidwa kutentha kwa nthawi yaitali (miyezi itatu);ikasungidwa pa 4 ° C, ntchito yake ndi kukhazikika kwake kudzakhala bwino, choncho ndi bwino kuti muzisunga pa 4 ° C, kumbukirani kuti musamasunge -20 ° C.
-Lysozyme ndi ufa wouma, womwe umagwira ntchito ukasungidwa pa 2-8 ° C kwa nthawi yaitali (miyezi itatu).Kuti musunge nthawi yayitali, chonde sungani pa -20°C.
