Magazi DNA Midi Kit (1-5ml) Magazi Kudzipatula Midi Kits kwa DNA kuchokera Magazi
Kufotokozera Kwa Zida:
Yeretsani mwachangu DNA yamtundu wapamwamba kwambiri kuchokera m'magazi a anticoagulated (1-5ml).
◮Palibe kuipitsidwa kwa RNase:DNA-Only Column yoperekedwa ndi zidayi imapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda kuwonjezera RNase panthawi yoyesera, kuletsa labotale kuti isaipitsidwe ndi RNase yachilendo.
◮Liwiro:Foregene Protease imakhala ndi zochita zambiri kuposa ma protease ofanana, ndipo imagaya zitsanzo za minofu mwachangu;ntchitoyo ndi yosavuta, ndipo ntchito yochotsa DNA ya genomic imatha kutha mkati mwa mphindi 20-80.
◮Zabwino:The centrifugation ikuchitika kutentha firiji, ndipo palibe chifukwa 4 ° C otsika kutentha centrifugation kapena Mowa mpweya wa DNA.
◮Chitetezo:Palibe organic reagent m'zigawo zofunika.
◮Mapangidwe apamwamba:DNA yotulutsidwa ya genomic ili ndi zidutswa zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira zosiyanasiyana.
◮Microelution System:Itha kuwonjezera kuchuluka kwa ma DNA amtundu, omwe ndi osavuta kuzindikira kapena kuyesa kuyesa.
Kufotokozera
Zitsanzo za magazi zimakhala ndi maselo ofiira ambiri a magazi ndi mapulateleti, zomwe zimapangitsa kuti ntchito ya magazi ikhale yovuta kwambiri.Komabe, magazi a genomic DNA otengedwa ndi njira wamba sakhala oyera komanso otsika kwambiri.Chidachi chimagwiritsa ntchito makina atsopano a Foregene Protease Plus ndi makina apadera a BL1 ndi BL2, omwe amatha kugayitsa magazi oletsa magazi komansocoagulationzitsanzo mu nthawi yaifupi kwambiri, potero kupewa kuwonongeka kwa DNA kwambiri ndikupeza kuchuluka kwakukulu kwa DNA ya genomic.Makina opangira magazi othamanga komanso osavuta opangira ma spin amathandizira kwambiri kuchotsa kwa ma genome amagazi, kotero kuti DNA yapamwamba kwambiri, yoyera kwambiri ya genomic imatha kupezeka mkati mwa mphindi 40.
DNA-yokha silica gel nembanemba ntchito pozungulira ndime ya zida izi ndi wapadera zinthu zatsopano za kampani, amene efficiently ndi makamaka adsorbs DNA, ndipo akhoza kuchotsa RNA, zonyansa mapuloteni, ayoni ndi zina organic mankhwala m'maselo pamlingo waukulu.Zidutswa za DNA zomwe zapezedwa ndi zazikulu, zoyera kwambiri, zokhazikika komanso zodalirika, ndipo mphamvu yonyamulira ya spin column ndi 80 μg DNA.DNA yomwe yapezedwa imatha kugwiritsidwa ntchito poyesa ma cell biology monga kuletsa chimbudzi, PCR, kusakanizidwa kwakum'mwera, kumanga laibulale ndi zina zotero.Chidacho chimatha kukonza mpaka 5ml ya magazi nthawi imodzi kuti ipeze kuchuluka kokwanira kwa DNA yoyera kwambiri ya genomic.
Zofotokozera
50 Preps, 100 Preps, 250 Preps
Zida zamagulu
| Mtengo wa BL1 |
| Bafa BL2* |
| Buffer DC |
| PW * |
| Mtengo WB1 |
| Mtengo wa EB |
| Foregene Protease Plus |
| Nambala ya DNA Yokha |
| Malangizo |
Mbali & ubwino
-Palibe kuipitsidwa kwa RNase: Gawo la DNA-Only loperekedwa ndi zidali limapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda kuwonjezera RNase panthawi yoyesera, kupewa labotale kuti isaipitsidwe ndi RNase yachilendo.
-Liwiro lofulumira: Foregene Protease Plus ili ndi zochita zambiri kuposa ma protease ofanana, ndipo imagaya zitsanzo za minofu mwachangu;ntchitoyo ndi yosavuta, ndipo ntchito yochotsa ma genomic DNA imatha kutha mkati mwa mphindi 40.
- Yabwino: The centrifugation ikuchitika kutentha firiji, ndipo palibe chifukwa 4°C kutentha pang'ono centrifugation kapena mpweya wa ethanol wa DNA.
-Safety: Palibe organic reagent m'zigawo zofunika.
-Zapamwamba kwambiri: DNA yotulutsidwa ya genomic ili ndi zidutswa zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira za mayesero osiyanasiyana.
Kit application
Ndiwoyenera kuchotsa ndi kuyeretsa DNA ya genomic kuchokera kumagazi atsopano kapena oundana omwe ali ndi anticoagulated.
Kayendedwe kantchito
Kusungirako ndi moyo wa alumali
-Zidazi zitha kusungidwa kwa miyezi 12 pansi pamikhalidwe yowuma kutentha kwapakati (15-25℃);ngati ikufunika kusungidwa kwa nthawi yayitali, ikhoza kusungidwa pa 2-8℃.
Zindikirani: Ngati atasungidwa kutentha kochepa, yankho limakhala lamvula.Musanagwiritse ntchito, onetsetsani kuti mwayika yankholo mu zida pa firiji kwakanthawi.Ngati n'koyenera, preheat mu madzi osamba 37 ° C kwa mphindi 10 kuti asungunuke mpweya, ndikusakaniza musanagwiritse ntchito.
-Foregene Protease Plus yankho lili ndi ndondomeko yapadera, yomwe imagwira ntchito ikasungidwa kutentha kwa nthawi yaitali (miyezi 3), ntchito yake ndi kukhazikika kwake kudzakhala bwino pamene kusungidwa pa 4 ° C, choncho tikulimbikitsidwa kusunga pa 4 ° C, kumbukirani kuti musasunge -20℃.
