Magazi a SuperDirectᵀᴹ PCR Kit-Heparin Magazi Direct PCR Master Mix for Genotyping of Blood
Kufotokozera Kwa Zida:
Mwachindunji gwiritsani ntchito magazi athunthu a heparin anticoagulated ngati template yakukulitsa PCR, yokhala ndi chidwi chachikulu komanso kuchita bwino kwambiri.
◮Palibe chifukwa choyeretsera DNA nthawi yambiri komanso yokwera mtengo, osakonzekera, komanso kugwiritsa ntchito magazi athunthu ngati template ya PCR.
◮Dongosololi lili ndi kuthekera kokulirapo ndipo limazindikira mwachidwi ma genome ndi zidutswa za DNA zomwe zikufuna m'magazi.
◮2 × SuperEasyTMMix-Heparin Kulekerera kwamagazi kwakukulu: mpaka 45% m'magazi a anthu ndi 20% m'magazi a mbewa.
◮Chitsanzocho chatsekedwa kwathunthu, kotero palibe chifukwa chodera nkhawa za kuipitsidwa kwachitsanzo ndi zotsatira zabodza za PCR.
Zofotokozera
200 × 20μl rxns, 2000 × 20μl rxns
2 × SuperEasyTMMix-Heparin: Ili ndi D-Taq DNA Polymerase yosinthidwa mwapadera, dNTPs, MgCl2, buffer reaction, PCR reaction enhancer, optimizer, stabilizer, ndi zina zotero. Pochita PCR, ingowonjezerani magazi athunthu, zoyambira, ndi ddH oyenera.2O ku 2 × SuperEasy yofananiraTMMix-Heparin kuti agwiritsidwe ntchito pakuchita kwa PCR.
6 × DNA Loading Buffer: The Loading Buffer ilibe SDS.Ndibwino kuti mugwiritse ntchito 6 × DNA Loading Buffer yophatikizidwa ndi zida pochita agarose gel electrophoresis kuti mupeze zotsatira zabwino za electrophoresis.Musagwiritse ntchito Loading Buffer yomwe ili ndi SDS, apo ayi padzakhala mchira waukulu wa kuwala kowala mumsewu panthawi ya electrophoresis, zomwe zidzakhudza zotsatira zoyesera.
Zida zamagulu
| 2 × SuperEasy TM Mix-Heparin |
| 6 × DNA Loading Buffer |
| Malangizo |
Mbali & ubwino
-Palibe chifukwa chotsuka nthawi komanso mtengo wamtengo wapatali wa DNA kuyeretsedwa, kusakonzekera, komanso kugwiritsa ntchito mwachindunji magazi athunthu monga PCR template.
-Dongosololi lili ndi kuthekera kokulirapo ndipo limatha kuzindikira mwachidwi ma genome ndi zidutswa za DNA zomwe zili m'magazi.
-2 × SuperEasyTMMix-Heparin Kulekerera kwamagazi kwakukulu: mpaka 45% m'magazi a anthu ndi 20% m'magazi a mbewa.
-Chitsanzocho chatsekedwa kwathunthu, kotero palibe chifukwa chodera nkhawa za kuipitsidwa kwachitsanzo ndi zotsatira zabodza za PCR.
Kit application
Wodzipereka ku chizindikiritso cha PCR cha Heparin anti-coagulated magazi athunthu.(Kuphatikiza: magazi a munthu, magazi a mbewa, magazi a nkhuku, magazi a mbalame, magazi a ng'ombe, magazi agalu, ndi zina zotero)
Kayendedwe kantchito
Kusungirako ndi moyo wa alumali
Chida ichi chiyenera kusungidwa pa -20 ℃.
2 × SuperEasyTMMix-Heparin, Ngati amagwiritsidwa ntchito kawirikawiri, amatha kusungidwa pa 4 ° C kuti asungidwe kwakanthawi kochepa (ntchito mkati mwa masiku 10).
6 × DNA Loading Buffer, Itha kusungidwa pa 4 ° C kapena -20 ° C kwa nthawi yayitali.
