Blood SuperDirectᵀᴹ PCR Kit(UNG)-EDTA Blood Direct PCR Master Mix ya Genotyping of Blood
Kufotokozera Kwa Zida:
Gwiritsani ntchito mwachindunji magazi athunthu a EDTA anticoagulated ngati template yokulitsa PCR, yokhala ndi chidwi chachikulu komanso kuchita bwino kwambiri.Dongosolo la UNG loletsa kuwononga chilengedwe lawonjezedwa kuti tipewe zolakwika.
◮Palibe chifukwa choyeretsera DNA nthawi yambiri komanso yokwera mtengo, osakonzekera, komanso kugwiritsa ntchito magazi athunthu ngati template ya PCR.
◮Dongosololi lili ndi kuthekera kokulirapo ndipo limazindikira mwachidwi ma genome ndi zidutswa za DNA zomwe zikufuna m'magazi.
◮2 × SuperEasyTMMix(UNG) -EDTA ili ndi kulolerana kwambiri kwa magazi: mpaka 45% m'magazi a anthu ndi 20% m'magazi a mbewa.
◮Chitsanzocho chatsekedwa kwathunthu, kotero palibe chifukwa chodera nkhawa za kuipitsidwa kwachitsanzo ndi zotsatira zabodza za PCR.Anti-pollution PCR system
◮2 × SuperEasyTMMix(UNG) -EDTA, imathetsa bwino kuipitsidwa ndi zinthu za PCR, ndikuwonetsetsa kutsimikizika ndi kulondola kwa kukulitsa.
Zofotokozera
200 × 20μl rxns, 2000 × 20μl rxns
2 × SuperEasyTMMix(UNG)-EDTA : Pamaziko a 2×SuperEasyTMMix-EDTA, dUTP imagwiritsidwa ntchito m'malo mwa dTTP, ndipo UNG enzyme yomwe ingawononge template yomwe ili ndi dUTP imawonjezedwa nthawi yomweyo, zomwe zingathe kuteteza kuipitsidwa kwa zinthu zokulitsa PCR.Muli D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer ndi stabilizer, ndi zina zotero. Mu PCR reaction, ingowonjezerani magazi athunthu, zoyambira, ndi ddH oyenera.2O ku 2 × SuperEasy yofananiraTMMix(UNG)-EDTA kuti igwiritsidwe ntchito pakuchita kwa PCR.
6 × DNA Loading Buffer: The Loading Buffer ilibe SDS.Ndibwino kuti mugwiritse ntchito 6 × DNA Loading Buffer yophatikizidwa ndi zida pochita agarose gel electrophoresis kuti mupeze zotsatira zabwino za electrophoresis.Musagwiritse ntchito Loading Buffer yomwe ili ndi SDS, apo ayi padzakhala mchira waukulu wa kuwala kowala mumsewu panthawi ya electrophoresis, zomwe zidzakhudza zotsatira zoyesera.
Zida zamagulu
| 2 × SuperEasyTMMix(UNG)-EDTA |
| 6 × DNA Loading Buffer |
| Malangizo |
Mbali & ubwino
- Palibe chifukwa choyeretsera DNA nthawi yambiri komanso yokwera mtengo, osakonzekera, komanso kugwiritsa ntchito magazi athunthu ngati template ya PCR.
-Dongosololi lili ndi kuthekera kokulirapo ndipo limatha kuzindikira mwachidwi ma genome ndi zidutswa za DNA zomwe zili m'magazi.
-2 × SuperEasyTMMix(UNG) -EDTA ili ndi kulolerana kwambiri kwa magazi: mpaka 45% m'magazi a anthu ndi 20% m'magazi a mbewa.
-Chitsanzocho chatsekedwa kwathunthu, kotero palibe chifukwa chodera nkhawa za kuipitsidwa kwachitsanzo ndi zotsatira zabodza za PCR.Anti-kuipitsa PCR dongosolo 2 × SuperEasyTMMix(UNG) -EDTA, imathetsa bwino kuipitsidwa ndi zinthu za PCR, ndikuwonetsetsa kutsimikizika ndi kulondola kwa kukulitsa.
Kit application
Wodzipereka ku chizindikiritso cha PCR cha EDTA anticoagulated magazi athunthu.(Kuphatikiza: magazi a munthu, magazi a mbewa, magazi a nkhuku, magazi a mbalame, magazi a ng'ombe, magazi agalu, ndi zina zotero)
Kayendedwe kantchito
Kusungirako ndi moyo wa alumali
Chida ichi chimasungidwa pa -20 ° C.
2 × SuperEasyTMMix(UNG)-EDTA，Ngati amagwiritsidwa ntchito pafupipafupi, amathanso kusungidwa pa 4°C kuti asungidwe kwakanthawi kochepa (ntchito mkati mwa masiku 10).
6 × DNA Loading Buffer, Itha kusungidwa pa 4 ° C kapena -20 ° C kwa nthawi yayitali.
