Magazi a SuperDirectᵀᴹ PCR Kit(UNG)-Heparin Magazi Direct PCR Master Mix for Genotyping of Blood
Zofotokozera
200 × 20μl rxns, 2000 × 20μl rxns
2 × SuperEasyTMMix (UNG) -Heparin: Pamaziko a 2 × SuperEasyTMMix-Heparin, dUTP imagwiritsidwa ntchito m'malo mwa dTTP, ndipo puloteni ya UNG yomwe ingawononge template ya dUTP imawonjezeredwa nthawi yomweyo, zomwe zingathe kuteteza kuipitsidwa kwa zinthu zokulitsa PCR.Muli D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer ndi stabilizer, ndi zina zotero. Mu PCR reaction, ingowonjezerani magazi athunthu, zoyambira, ndi ddH oyenera.2O ku 2 × SuperEasy TM Mix (UNG) -Heparin yogwiritsidwa ntchito pa PCR.
6 × DNA Loading Buffer: The Loading Buffer ilibe SDS.Ndibwino kuti mugwiritse ntchito 6 × DNA Loading Buffer yophatikizidwa ndi zida pochita agarose gel electrophoresis kuti mupeze zotsatira zabwino za electrophoresis.Musagwiritse ntchito Loading Buffer yomwe ili ndi SDS, apo ayi padzakhala mchira waukulu wa kuwala kowala mumsewu panthawi ya electrophoresis, zomwe zidzakhudza zotsatira zoyesera.
Zida zamagulu
2 × SuperEasyTMMix(UNG)-Heparin |
6 × DNA Loading Buffer |
Malangizo |
Mbali & ubwino
-Dongosololi lili ndi kuthekera kokulirapo ndipo limazindikira mwachidwi ma genome ndi zidutswa za DNA zomwe zikufuna m'magazi.
-2 × SuperEasyTMMix(UNG) -Heparin Kulekerera kwakukulu kwa magazi: mpaka 45% m'magazi aumunthu ndi 20% m'magazi a mbewa.
-Chitsanzocho chatsekedwa kwathunthu, kotero palibe chifukwa chodera nkhawa za kuipitsidwa kwachitsanzo ndi zotsatira zabodza za PCR.Anti-kuipitsa PCR dongosolo 2 × SuperEasyTMSakanizani (UNG), kuthetsa bwino kuipitsidwa komwe kumachitika ndi zinthu za PCR, ndikuwonetsetsa kutsimikizika ndi kulondola kwa kukulitsa.
Kit application
Odzipereka ku chizindikiritso cha PCR cha Heparin anticoagulated magazi athunthu.(Kuphatikiza: magazi a munthu, magazi a mbewa, magazi a nkhuku, magazi a mbalame, magazi a ng'ombe, magazi agalu, ndi zina zotero)
Kayendedwe kantchito
Kusungirako ndi moyo wa alumali
Njira yonse yamayendedwe otsika otsika oundana, kuonetsetsa kuti zida zili m'malo a <4 ℃.
2 × SuperEasyTMMix(UNG) -Heparin, ngati imagwiritsidwa ntchito kawirikawiri, imathanso kusungidwa pa 4 ° C kuti isungidwe kwakanthawi kochepa (ntchito mkati mwa masiku 10).
6 × DNA Loading Buffer, ikhoza kusungidwa pa 4 ° C kapena -20 ° C kwa nthawi yaitali.