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Buccal Swab/FTA Card DNA Isolation Kit Genomic DNA Extraction kapena Purifiction Kit kuchokera ku Buccal Swabs

Kufotokozera Kwa Zida:

Yeretsani mwachangu ma genomic DNA kuchokera ku zitsanzo za buccal swab/FTA Card.

Palibe kuipitsidwa kwa RNase:DNA-Only Column yoperekedwa ndi zidayi imapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda kuwonjezera RNase panthawi yoyesera, kuletsa labotale kuti isaipitsidwe ndi RNase yachilendo.

Liwiro:Foregene Protease imakhala ndi zochita zambiri kuposa ma protease ofanana, ndipo imagaya zitsanzo za minofu mwachangu;ntchitoyo ndi yosavuta, ndipo ntchito yochotsa DNA ya genomic imatha kutha mkati mwa mphindi 20-80.

Zabwino:The centrifugation ikuchitika kutentha firiji, ndipo palibe chifukwa 4 ° C otsika kutentha centrifugation kapena Mowa mpweya wa DNA.

Chitetezo:Palibe organic reagent m'zigawo zofunika.

Mapangidwe apamwamba:DNA yotulutsidwa ya genomic ili ndi zidutswa zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira zosiyanasiyana.

Microelution System:Itha kuwonjezera kuchuluka kwa ma DNA amtundu, omwe ndi osavuta kuzindikira kapena kuyesa kuyesa.

mphamvu zam'tsogolo


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

FAQ

Kufotokozera

Chidachi chimapereka njira yabwino komanso yofulumira yopezera ma DNA okwera kwambiri kuchokera ku buccal swabs ndi FTA Card (madontho amagazi).Kugwiritsa ntchito kampani yathu's yapadera ya DNA-yokha silica membrane spin column ndi formula, yophatikizidwa ndi Foregene Protease, high-concentration, high-concentration, high-quality genomic DNA ikhoza kuchotsedwa mu mphindi 80.Gawo laling'ono loyeretsedwa lopangidwa mwapadera limamanga DNA ya genomic, ndipo DNA imatha kuchotsedwa pang'ono (15μl) elution system kuti muwonjezere kuchuluka kwa ma genomic DNA omwe amapezeka, omwe ndi osavuta kuzindikirika kapena kuyesa kutsika.Chidacho chikhoza kukonza chitsanzo chimodzi kapena zingapo panthawi imodzi, ndipo kuyeretsa sikufuna kuchotsa zinthu zakuthupi monga phenol, chloroform, ndi isopropanol kapena mpweya wa ethanol, ndipo ntchitoyo ndi yosavuta komanso yopulumutsa nthawi.

Zofotokozera

50 Zokonzekera

Zida zamagulu

Mtengo wa ST1

Mtengo wa ST2
 Linear Acrylamide
Mtengo PW
Mtengo WB
Mtengo wa EB
 Foregene Protease
Nambala ya DNA Yokha

Malangizo

Mbali & ubwino

-Palibe kuipitsidwa kwa RNase: Gawo la DNA-Only loperekedwa ndi zidali limapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda kuwonjezera RNase panthawi yoyesera, kupewa labotale kuti isaipitsidwe ndi RNase yachilendo.

-Kuthamanga Kwambiri: Foregene Protease ili ndi zochita zambiri kuposa ma protease ofanana, imagaya zitsanzo mwachangu;ntchito yosavuta.

- Yabwino: The centrifugation ikuchitika kutentha firiji, ndipo palibe chifukwa 4 ° C otsika kutentha centrifugation kapena Mowa mpweya wa DNA.

-Safety: Palibe organic reagent m'zigawo zofunika.

-Zapamwamba kwambiri: DNA yotulutsidwa ya genomic ili ndi zidutswa zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira za mayesero osiyanasiyana.

-Micro-elution system: Itha kuwonjezera kuchuluka kwa ma DNA a genomic, omwe ndi osavuta kuzindikira kapena kuyesa kutsika.

Kit application

Ndizoyenera kuyeretsa DNA ya genomic kuchokera ku zitsanzo zotsatirazi: buccal swabs, FTA Card (madontho a magazi).

Kusungirako ndi moyo wa alumali

-Chida ichi chikhoza kusungidwa kwa miyezi 12 pansi pa nyengo youma kutentha (15-25 ° C);Ngati ikufunika kusungidwa kwa nthawi yayitali, imatha kusungidwa pa 2-8 ° C.

Zindikirani: Ngati atasungidwa kutentha kochepa, yankho limakhala lamvula.Musanagwiritse ntchito, onetsetsani kuti mwayika yankholo mu zida pa firiji kwakanthawi.Ngati n'koyenera, preheat mu madzi osamba 37 ° C kwa mphindi 10 kuti asungunuke mpweya, ndikusakaniza musanagwiritse ntchito.

-Foregene Protease yankho lili ndi ndondomeko yapadera, yomwe imagwira ntchito ikasungidwa kutentha kwa nthawi yaitali (miyezi itatu);ntchito yake ndi kukhazikika kwake kudzakhala bwino pamene kusungidwa pa 4 ° C, choncho tikulimbikitsidwa kusunga pa 4 ° C, kumbukirani kuti musamasunge -20 ° C.


  • Zam'mbuyo:
  • Ena:

  • Mzere woyeretsa watsekedwa

    Mu zida izi, mu genomic DNA m'zigawo ntchito, ndime kuyeretsedwa mwachindunji adsorbed pa chitsanzo enzymatic lysis osakaniza popanda sitepe centrifugation, ndi ndime kuyeretsedwa akhoza oletsedwa chifukwa chosakwanira enzymization ndi mkulu mamasukidwe akayendedwe a chitsanzo.

    Zifukwa zotsatirazi ndi izi:

    1. Kusakwanira kwa enzymatic chimbudzi cha zitsanzo za minofu.

    Malangizo: Zitsanzo za nthawi yopangira Foregene Protease zitha kukulitsidwa moyenerera kapena supernatant ingatengedwe pambuyo pa centrifugation pa 12,000 rpm (~13,400 × g) kwa 5 min.

    2. Kugwiritsa ntchito kwambiri zitsanzo za minofu kapena minofu yaikulu.

    Malangizo: Ndibwino kuti musapitirire 1 Buccal swab mu chitsanzo;ngati chitsanzocho ndi chachikulu kwambiri, onjezani mlingo wa Buffer ST1, Foregene Protease, buffer ST2 moyenerera.

    3. Kukhuthala kwachitsanzo ndikokwera kwambiri.

    Malangizo: Zitsanzo zitha kuchepetsedwa moyenera ndi 10 mM ya Tris-HCl musanayambe kutulutsa ma genomic DNA.

    4. Zidutswa za khadi la magazi zayamwa.

    Malangizo: Nthawi yocheperako ya centrifugation ya sitepe 6 ya malo amagazi (FTA Card) yotulutsa ma genomic imatha kukulitsidwa moyenerera.

    Zokolola zochepa kapena palibe DNA

    Nthawi zambiri pamakhala zinthu zosiyanasiyana zomwe zimakhudza zokolola za DNA ya genomic, kuphatikizapo chiyambi cha zitsanzo, mikhalidwe yosungiramo zitsanzo, kukonzekera zitsanzo, kusintha, ndi zina zotero.

    Genomic DNA sichingapezeke panthawi yochotsa

    Zomwe zingatheke ndi izi:

    1. Kusungidwa molakwika kwa zitsanzo kapena kusungidwa kwa nthawi yayitali kumabweretsa kuwonongeka kwa ma genomic DNA.

    Malangizo: Masamba a pakamwa amayenera kutsatiridwa mwatsopano, ndipo sikoyenera kugwiritsa ntchito swabs zosungidwa pochotsa ma genomic DNA;zitsanzo za malo a magazi ziyenera kuonetsetsa kuti khalidweli ndi loyenerera ndipo nthawi yosungira sikuyenera kukhala yaitali kwambiri.

    2. Kugwiritsa ntchito minofu pang'ono kungapangitse kuti DNA ya ma genomic isatuluke.

    Malangizo: Tsatirani malangizo a buccal swab mu bukhu la opareshoni, ndipo pukutani kangapo momwe mungathere kuti ma cell okwanira amangiridwe ku swab yapakamwa kuti achotse ma genomic DNA;pochotsa magazi, malo odulira magazi amatha kuonjezedwa moyenerera.

    3. Foregene Protease imasungidwa molakwika, zomwe zimapangitsa kuchepa kwa ntchito yake kapena kusakhazikika.

    Maupangiri: Tsimikizirani zosungirako za Foregene Protease kapena m'malo mwake ndi Foregene Protease yatsopano ya ma enzymatic reaction.

    4. Kusungidwa kosayenera kwa zida kapena nthawi yosungirako ndi nthawi yayitali kwambiri, zomwe zimapangitsa kuti zigawo zina ziwonongeke muzitsulo.

    Malangizo: Gulani zida zatsopano zodzipatula za Buccal swab DNA kuti zigwirizane nazo.

    5. Buffer WB sichiwonjezera ethanol.

    Malangizo: Tsimikizirani kuti buffer WB imawonjezera voliyumu yolondola ya ethanol.

    6. Chosowacho sichinawonjezedwe molondola ku filimu ya silikoni.

    Malangizo: Onjezani madontho a 65 ° C otenthedwa asanatenthedwe pakati pa nembanemba ya silikoni ndikusiya kutentha kwa mphindi 5 kuti muwonjezere mphamvu.

    Low-zokolola genomic DNA yokha

    Zifukwa zotsatirazi ndi izi:

    1. Kusungidwa molakwika kwa zitsanzo kapena kusungidwa kwa nthawi yayitali kumabweretsa kuwonongeka kwa ma genomic DNA.

    Malangizo: Masamba amkamwa amasankhidwa mwatsopano, ndipo ma swabs osungidwa sayenera kugwiritsidwa ntchito pochotsa ma genomic DNA.

    2. Ngati kuchuluka kwa minofu kuli kochepa kwambiri, zomwe zatulutsidwa mu DNA zimakhala zochepa.

    Malangizo: Tsatirani malangizo a oral swab mu bukhu la opareshoni, pukutani kangapo momwe mungathere kuti ma cell okwanira amangiridwe pakamwa kuti muchotse DNA ya genomic.

    3. Foregene Protease imasungidwa molakwika, zomwe zimapangitsa kuchepa kwa ntchito yake kapena kusakhazikika.

    Maupangiri: Tsimikizirani zosungirako za Foregene Protease kapena m'malo mwake ndi Foregene Protease yatsopano ya ma enzymatic reaction.

    4. Mavuto osaneneka.

    Malangizo: Gwiritsani ntchito Buffer EB kwa elution;ngati mukugwiritsa ntchito ddH2O kapena zofotokozera zina, zimatsimikizira kuti pH ya eluate ili pakati pa 7.0-8.5.

    5. The eluate sanawonjezedwe molondola dropwise.

    Malangizo: Onjezani madontho osawoneka bwino pakati pa nembanemba ya silikoni ndikusiya kutentha kwa mphindi 5 kuti muwonjezere mphamvu.

    6. Madzi a elution amawunjikana pang'ono.

    Malangizo: Gwiritsani ntchito eluent pakuwunika kwa ma genomic DNA monga momwe akufunira mu malangizo, osachepera 15 μl.

    Ochepa chiyero cha genomic DNA okha

    Low genomic DNA chiyero kungayambitse kulephera kapena zotsatira zosakhutiritsa za zoyesera zotsika, monga: ma enzyme sangatsegulidwe, PCR satha kupeza gawo lachidwi, ndi zina zambiri.

    Zomwe zingatheke ndi izi:

    1. Heteroprotein kuipitsa, RNA kuipitsa.

    Kusanthula: Mzere woyeretsedwa sunatsukidwe pogwiritsa ntchito Buffer PW;ndime yoyeretsa ya Buffer PW sinatsukidwe pogwiritsa ntchito liwiro lolondola la centrifugal.

    Malangizo: Onetsetsani kuti palibe mvula mumphamvu yamphamvu musanawonjezere Mowa;onetsetsani kuti mukutsuka ndime yoyeretsera molingana ndi malangizo, ndipo sitepe iyi siyingasiyidwe.

    2. Kudetsedwa kwa ayoni.

    Kuwunika: Mzere woyeretsera wa Buffer WB sunasiyidwe kapena kutsukidwa kamodzi kokha, zomwe zidapangitsa kuipitsidwa kwa ayoni.

    Malangizo: Onetsetsani kuti mukutsuka Buffer WB ka 2 monga mwalangizidwa kuti muchotse ma ion otsalira momwe mungathere.

    3. Kuwonongeka kwa enzyme ya RNA.

    Kusanthula: Ma RNase akunja adawonjezedwa ku buffer;Opaleshoni yotsuka ya Buffer PW inali yolakwika, zomwe zidapangitsa kuti zotsalira za RNase, zomwe zikukhudza zoyeserera zakutsika za RNA, monga zolembera mu vitro.

    Malangizo: Foregene mndandanda nucleic acid kudzipatula zida akhoza kuchotsa RNA popanda kuwonjezera zina RNase, motero buccal Swab/FTA Khadi DNA Kudzipatula Kit sayenera kuwonjezera RNase;onetsetsani kuti mwatsatira malangizo a gawo la kuyeretsedwa kwa Buffer PW, ndipo sitepe iyi siyingasiyidwe.

    4. Zotsalira za Ethanol.

    Kusanthula: Buffer WB sanachite kanthu chubu centrifugation atatsuka ndime yoyeretsa.

    Malangizo: Chitani olondola opanda kanthu chubu centrifugation ntchito malinga ndi malangizo.

    5. Kuipitsa kwina konyansa.

    Kusanthula: Zitsanzo zosungidwa kapena zitsanzo zapadera sizimakonzedweratu.

    Malangizo: Yang'anirani bwino chitsanzo monga mwalangizidwa.

    Lembani uthenga wanu apa ndikutumiza kwa ife

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