-
Cell Direct RT qPCR zida
Chida ichi chimagwiritsa ntchito pulogalamu yapadera ya lysis yomwe imatha kutulutsa RNA mwachangu pamaselo otukuka amachitidwe a RT-qPCR, potero ndikuchotsa kuyeretsa kwa RNA komwe kumawononga nthawi yambiri komanso kotopetsa. Chithunzi cha RNA chitha kupezeka mu mphindi 7 zokha. The 5 × Direct RT Mix ndi 2 × Direct qPCR Mix-SYBR reagents zoperekedwa ndi zida zitha kupeza mwachangu komanso moyenera zotsatira za PCR zenizeni zenizeni.
5 × Direct RT Mix ndi 2 × Direct qPCR Mix-SYBR ali ndi mphamvu yolekerera zoletsa, ndipo lysate yazitsanzo zitha kugwiritsidwa ntchito ngati template ya RT-qPCR mwachindunji. Chikwamachi chili ndi RNA yodziwika bwino kwambiri ya Foregene reverse transcriptase, ndi Hot D-Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer ndi stabilizer.
