Cell Total RNA Isolation Kit

Kufotokozera Kwa Zida:

RNase-Free

Imagwiritsidwa ntchito kutentha (15-25 ℃)

Ndi DNA-Cleaning Column

Mtengo wapatali wa magawo RNA

Fast: Malizitsani kutulutsa mu mphindi 11

Chitetezo: Palibe Organic Chemical

foregene strength


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  • Tsatanetsatane wa Zamalonda

    Zogulitsa Tags

    Kufotokozera

    50 Preps, 200 Preps

    Chidachi chimagwiritsa ntchito spin column ndi formula yopangidwa ndi Foregene, yomwe imatha kutulutsa RNA yapamwamba komanso yapamwamba kwambiri kuchokera ku ma cell opangidwa mu mbale 96, 24, 12, ndi 6-zitsime.

    Chidachi chimapereka DNA-Cleaning Column yogwira mtima, yomwe imatha kulekanitsa ma supernatant ndi cell lysate, kumanga ndi kuchotsa DNA ya genomic.Ntchitoyi ndi yosavuta komanso yopulumutsa nthawi.

    Tiye RNA-Column yokha imatha kumangirira RNA ndi njira yapadera.Zitsanzo zambiri zimatha kukonzedwa nthawi imodzi.

    Zida zamagulu

    Kupanga kwa zida RE-03111 RE-03114
    50 T 200 T
    Buffer cRL1* 25 ml pa 100 ml
    Mtengo wa cRL2 15 ml pa 60 ml pa
    Bafa RW1* 25 ml pa 100 ml
    Mtengo wa RW2 24 ml pa 96 ml pa
    RNase-Free ddH2O 10 ml pa 40 ml pa
    RNA-Only Column 50 200
    DNA-Kuyeretsa Mzere 50 200
    Malangizo 1 1

    *Chonde valani magolovesi ndikuchitapo kanthu zodzitchinjiriza mukugwira ntchito popeza Buffer cRL1 ndi Buffer RW1 zili ndi mchere wachaotropic wokwiyitsa.

    Mbali&zabwino

    ■ Njira yonseyi imayendetsedwa ndi kutentha kwa chipinda (15-25 ℃), popanda kusamba kwa ayezi ndi kutentha kochepa kwa centrifugation.
    ■ Zida zonse ndi RNase-Free, palibe chifukwa chodera nkhawa za kuwonongeka kwa RNA.
    ■ DNA-Cleaning Column imamangiriza DNA, kotero kuti zida zitha kuchotsa kuipitsidwa kwa DNA popanda kuwonjezera DNase.
    ■ Zokolola zambiri za RNA: Mzere wa RNA-okha ndi fomula yapadera imatha kuyeretsa RNA bwino.
    ■ Kuthamanga kwachangu: kosavuta kugwiritsa ntchito ndipo kumatha kutha mphindi 11.
    ■ Chitetezo: Palibe organic reagent yomwe imafunikira.
    ■ Ubwino wapamwamba: RNA yoyeretsedwa ndi yoyera kwambiri, yopanda mapuloteni ndi zonyansa zina, ndipo imatha kukumana ndi mayesero osiyanasiyana.

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    Kit application

    Ndiwoyenera kuchotsa ndi kuyeretsa RNA yonse kuchokera ku maselo otukuka mu mbale 96, 24, 12, ndi 6-chitsime.

    Kuyenda kwa ntchito

    cell total RNA

    Chithunzi

    Cell Total RNA Isolation Kit Work Flow1

    The agarose gel osakaniza batire chithunzi cha Cell Total RNA Isolation Kit ankachitira pamwamba manambala osiyanasiyana a maselo, 20μl voliyumu elution, kutenga 2μl oyeretsedwa okwana RNA 1%.

    Kusungirako ndi moyo wa alumali

    Zida zitha kusungidwa kwa miyezi 12 kutentha kwa chipinda (15-25 ℃) kapena 2-8 ℃ kwa nthawi yayitali (miyezi 24).

    Buffer cRL1 ikhoza kusungidwa pa 4 ℃ kwa mwezi umodzi mutawonjezera 2-hydroxy-1-ethanethiol(posankha).


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