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Cell Yonse ya RNA Yodzipatula
Chikwama ichi chimagwiritsa ntchito gawo loyambira ndi chilinganizo chopangidwa ndi Foregene, chomwe chimatha kuchotsa kuyera kwapamwamba komanso kwapamwamba kwambiri kwa RNA yonse kuchokera kumaselo opangidwa mu 96, 24, 12, ndi 6-mbale zabwino. Chikwamacho chimapereka gawo loyeserera la DNA-loyeretsa, lomwe limatha kusiyanitsa supernatant ndi cell lysate, kumangirira ndikuchotsa genomic DNA. Ntchitoyi ndi yosavuta komanso yopulumutsa nthawi; Column yokhayo ya RNA imatha kumangiriza RNA mwanjira yapadera. Ziwerengero zambiri zitha kukonzedwa nthawi imodzi.
Dongosolo lonse ndilopanda RNase, kotero kuti RNA yoyeretsedwa isanyozedwe; Buffer RW1, Buffer RW2 chotsuka chomenyera chotetezera chimapatsa RNA yopanda mapuloteni, DNA, ion, ndi kuipitsa kwamagulu.
