FFPE DNA Isolation Kit DNA Extraction Purification Kit kuchokera ku FFPE Zitsanzo
Kufotokozera Kwa Zida:
Yeretsani mwachangu ma DNA amtundu wapamwamba kwambiri kuchokera kuzinthu zophatikizika ndi parafini monga magawo a parafun ndi mabulogu a parafini.
◮Palibe kuipitsidwa kwa RNase:DNA-Only Column yoperekedwa ndi zidayi imapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda kuwonjezera RNase panthawi yoyesera, kuletsa labotale kuti isaipitsidwe ndi RNase yachilendo.
◮Liwiro:Foregene Protease imakhala ndi zochita zambiri kuposa ma protease ofanana, ndipo imagaya zitsanzo za minofu mwachangu;ntchitoyo ndi yosavuta, ndipo ntchito yochotsa DNA ya genomic imatha kutha mkati mwa mphindi 20-80.
◮Zabwino:The centrifugation ikuchitika kutentha firiji, ndipo palibe chifukwa 4 ° C otsika kutentha centrifugation kapena Mowa mpweya wa DNA.
◮Chitetezo:Palibe organic reagent m'zigawo zofunika.
◮Mapangidwe apamwamba:DNA yotulutsidwa ya genomic ili ndi zidutswa zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira zosiyanasiyana.
◮Microelution System:Itha kuwonjezera kuchuluka kwa ma DNA amtundu, omwe ndi osavuta kuzindikira kapena kuyesa kuyesa.
Kufotokozera
Zidazi zimaperekedwa kuti zichotse ndikuyeretsa DNA yamtundu wapamwamba kwambiri kuchokera ku minofu yokhazikika ya formaldehyde kapena minofu yokhala ndi parafini.
DNA ya genomic ya chitsanzo chophatikizidwa ndi parafini idzawonongeka chifukwa cha nthawi yosungirako kapena chilengedwe, zomwe zimapangitsa kuti zikhale zovuta kuchotsa DNA ya genomic.Chidachi chimagwiritsa ntchito njira yapadera yokongoletsedwa, yomwe ili yoyenera kuchotsa DNA ya genomic kuchokera ku zitsanzo zokhala ndi parafini ndikuchita bwino kwambiri.Njira yonseyi sifunika kugwiritsa ntchito phenol, chloroform kapena mpweya wa isopropanol.
Chidachi chimagwiritsa ntchito zozungulira zomwe zimatha kumangirira DNA, Foregene Protease Plus yatsopano, komanso makina apadera achitetezo, omwe amatha kutulutsa DNA yapamwamba kwambiri ya parafini mkati mwa maola 2-5.
Zofotokozera
50 Preps, 100 Preps, 250 Preps
Zida zamagulu
| Mtengo FL1 |
| Bafa FL2* |
| PW * |
| Mtengo WB |
| Mtengo wa EB |
| Foregene Protease Plus |
| Nambala ya DNA Yokha |
| Malangizo |
Mbali & ubwino
-Palibe kuipitsidwa kwa RNase: Gawo la DNA-Only loperekedwa ndi zidali limapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda kuwonjezera RNase panthawi yoyesera, kupewa labotale kuti isaipitsidwe ndi RNase yachilendo.
-Kuthamanga Kwambiri: Foregene Protease Plus ili ndi ntchito zambiri kuposa ma protease ofanana, ndipo imagaya zitsanzo za minofu mofulumira;Opaleshoniyo ndi yosavuta, ndipo ntchito yochotsa ma genomic DNA imatha kutha mkati mwa maola 2-5.
-Kuthandiza: The centrifugation ikuchitika kutentha firiji, ndipo palibe chifukwa 4°C kutentha pang'ono centrifugation kapena mpweya wa ethanol wa DNA.
- Chitetezo: palibe organic reagent m'zigawo zofunika.
-Zapamwamba: Zidutswa za DNA zomwe zatulutsidwa ndi zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira pazoyeserera zosiyanasiyana.
Kit application
Ndiwoyenera kuchotsa ndi kuyeretsa DNA ya genomic kuchokera ku zitsanzo zokhazikika za formaldehyde ndi zitsanzo za minofu ya parafini.
Kayendedwe kantchito
Kusungirako ndi moyo wa alumali
-Chida ichi chikhoza kusungidwa kwa miyezi 12 pansi pa nyengo youma kutentha (15-25 ° C);Ngati ikufunika kusungidwa kwa nthawi yayitali, imatha kusungidwa pa 2-8 ° C.
Zindikirani: Ngati atasungidwa kutentha kochepa, yankho limakhala lamvula.Musanagwiritse ntchito, onetsetsani kuti mwayika yankholo mu zida pa kutentha kwapakati kwa nthawi.Ngati n'koyenera, preheat mu madzi osamba 37 ° C kwa mphindi 10 kuti asungunuke mpweya, ndikusakaniza musanagwiritse ntchito.
-Foregene Protease Plus yankho lili ndi njira yapadera yomwe imagwira ntchito ikasungidwa kutentha kwa nthawi yayitali (miyezi itatu);imakhala yogwira ntchito komanso yosasunthika ikasungidwa pa 4 ° C, choncho ndi bwino kuisunga pa 4 ° C, kumbukirani kuti musaisunge pa -20 ° C.
