Direct PCR

  • Animal Tissue Direct PCR kit

    Matenda a Animal Direct PCR kit

    Chida ichi chimagwiritsa ntchito chida chapadera cha lysis kuti amasule mwachangu ma genomic a DNA kuchokera ku mitundu yazinyama ya PCR, chifukwa chake ndioyenera kwambiri kuyesa kwakukulu kwa majini.

    Njira yotulutsa genomic DNA kuchokera ku lysis buffer imamalizidwa mkati mwa 10-30 mphindi pa 65°C. Palibe njira zina monga kuchotsa mapuloteni ndi RNA zofunika, ndipo DNA yomwe ingafalitsidwe itha kugwiritsidwa ntchito ngati template ya PCR reaction.

    2× PCR YosavutaTM Sakanizani muli ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo mutha kugwiritsa ntchito lysate yachitsanzo kuti ayesedwe ngati template yolimbikitsira bwino. Reagent iyi ili ndi Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer ndi kukhazikika.

    D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.

  • Mouse Tail SuperDirect PCR Kit

    Mbewa Mchira SuperDirect PCR Kit

    Chida ichi chimagwiritsa ntchito njira yapadera ya lysis buffer kuti imasule mwachangu ma genomic a DNA kuchokera kumiyendo ya mbewa ndi mbewa zamakutu kuti PCR ichitepo kanthu, motero ndioyenera makamaka kuyesa kwakukulu kwa majini.

    Njira yotulutsa genomic DNA kuchokera ku lysis buffer yatha mkati mwa mphindi 10-30 kutentha kwapakati (20-25 ° C). Palibe kutenthetsa komwe kumafunikira, ndipo njira zina monga kuchotsa mapuloteni ndi RNA sizofunikira. Zambiri zomwe zatulutsidwa za DNA zitha kugwiritsidwa ntchito ngati template ya PCR reaction.

    2 × M-PCR Easy TM Mix ili ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo itha kugwiritsa ntchito lysate yachitsanzo kuti iyesedwe ngati template yolimbikitsira bwino. Reagent iyi ili ndi Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer ndi stabilizer.

    D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.

  • Animal Tissue Direct PCR kit-UNG

    Zilonda Zanyama Direct PCR kit-UNG

    Chida ichi chimagwiritsa ntchito chida chapadera cha lysis kuti amasule mwachangu ma genomic a DNA kuchokera ku mitundu yazinyama ya PCR, chifukwa chake ndioyenera kwambiri kuyesa kwakukulu kwa majini.

    Njira yotulutsa genomic DNA kuchokera ku lysis buffer imamalizidwa mkati mwa 10-30 mphindi pa 65°C. Palibe njira zina monga kuchotsa mapuloteni ndi RNA zofunika, ndipo DNA yomwe ingafalitsidwe itha kugwiritsidwa ntchito ngati template ya PCR reaction.

    2×PCR YosavutaTM Mix (UNG) imagwiritsa ntchito DUTP m'malo mwa dTTP pamaziko a 2×PCR YosavutaTM Sakanizani, ndikuwonjezera enzyme ya UNG (Uracil-N-glycosylase) yomwe imatha kutsitsa template yomwe ili ndi DUTP nthawi yomweyo. Asanachitike PCR, puloteni ya UNG imagwiritsidwa ntchito kunyoza zomwe PCR imakhala ndi uracil. Enzyme ya UNG sikhala ndi tanthauzo lililonse pazomwe mulibe uracil, potero kuwonetsetsa kuti kulimbitsa ndikulondola ndikulondola ndikuletsa kuthekera kwa kuipitsidwa kwa zinthu za PCR pakuyesa kwamitundu yayikulu.

    D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.

  • Cell Direct RT qPCR Kit

    Cell Direct RT qPCR zida

    Chida ichi chimagwiritsa ntchito pulogalamu yapadera ya lysis yomwe imatha kutulutsa RNA mwachangu pamaselo otukuka amachitidwe a RT-qPCR, potero ndikuchotsa kuyeretsa kwa RNA komwe kumawononga nthawi yambiri komanso kotopetsa. Chithunzi cha RNA chitha kupezeka mu mphindi 7 zokha. The 5 × Direct RT Mix ndi 2 × Direct qPCR Mix-SYBR reagents zoperekedwa ndi zida zitha kupeza mwachangu komanso moyenera zotsatira za PCR zenizeni zenizeni.

    5 × Direct RT Mix ndi 2 × Direct qPCR Mix-SYBR ali ndi mphamvu yolekerera zoletsa, ndipo lysate yazitsanzo zitha kugwiritsidwa ntchito ngati template ya RT-qPCR mwachindunji. Chikwamachi chili ndi RNA yodziwika bwino kwambiri ya Foregene reverse transcriptase, ndi Hot D-Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer ndi stabilizer.

  • Mouse Tail Direct PCR Kit

    Mbewa Mchira Direct PCR Kit

    Chida ichi chimagwiritsa ntchito njira yapadera ya lysis kuti imasule mwachangu ma genomic a DNA kuchokera kumiyendo ya mbewa, makutu, minofu ndi mitundu ina ya minyewa yamachitidwe a PCR, chifukwa chake ndioyenera makamaka kuyesa kwakukulu kwa majini.

    Njira yotulutsa genomic DNA kuchokera ku lysis buffer imamalizidwa mkati mwa 10-30 mphindi pa 65 ° C. Palibenso njira zina monga kuchotsa mapuloteni ndi RNA, ndipo DNA yomwe ingatulutsidwe itha kugwiritsidwa ntchito ngati template ya PCR reaction.

    2 × M1-PCR Easy TM Mix ili ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo itha kugwiritsa ntchito lysate yachitsanzo kuti iyesedwe ngati template yolimbikitsira bwino. Reagent iyi ili ndi Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer ndi stabilizer.

    D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.

  • Mouse Tail Direct PCR Kit-UNG

    Mbewa Mchira Direct PCR zida-UNG

    Chida ichi chimagwiritsa ntchito njira yapadera ya lysis kuti imasule mwachangu ma genomic a DNA kuchokera kumiyendo ya mbewa, makutu, minofu ndi mitundu ina ya minyewa yamachitidwe a PCR, chifukwa chake ndioyenera makamaka kuyesa kwakukulu kwa majini.

    Njira yotulutsa genomic DNA kuchokera ku lysis buffer imamalizidwa mkati mwa 10-30 mphindi pa 65 ° C. Palibenso njira zina monga kuchotsa mapuloteni ndi RNA, ndipo DNA yomwe ingatulutsidwe itha kugwiritsidwa ntchito ngati template ya PCR reaction.

    2 × M1-PCR Easy TM Mix (UNG) imagwiritsa ntchito DUTP m'malo mwa dTTP pamaziko a 2 × M1-PCR Easy TM Mix (UNG), ndipo imawonjezera enzyme ya UNG (Uracil-N-glycosylase) yomwe imatha kutsitsa template yomwe ili ndi DUTP ku nthawi yomweyo. Asanachitike PCR, puloteni ya UNG imagwiritsidwa ntchito kunyoza zomwe PCR imakhala ndi uracil. Enzyme ya UNG sikhala ndi zotsatirapo pazomwe sizikhala ndi uracil, potero zimatsimikizira kulondola ndi kulondola kwa kukulitsa ndikuletsa kuthekera kwa kuyesedwa kwamitundu yayikulu. Kuwonongeka kwa zinthu za PCR kunachitika.

    D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.

  • Zebra Fish Direct PCR Kit

    Mbidzi Nsomba Direct PCR Kit

    Chida ichi chimagwiritsa ntchito njira yapadera yolumikizira lysis kutulutsa mwachangu genomic kuchokera ku zebrafish ndi ziwombankhanga zina zamadzi amchere, zipsepse za mchira kapena zitsanzo za mazira a nsomba pakuyankha kwa PCR, kotero ndizoyenera makamaka kuyesa kwakukulu kwa majini. Njira yotulutsa genomic DNA kuchokera ku lysis buffer imamalizidwa mkati mwa 10-30 mphindi pa 65 ° C. Palibenso njira zina monga kuchotsa mapuloteni ndi RNA, ndipo DNA yomwe ingatulutsidwe itha kugwiritsidwa ntchito ngati template ya PCR reaction.

    2 × PCR Easy TM Mix ili ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo itha kugwiritsa ntchito lysate ya nyemba kuti iyesedwe ngati template yolimbikitsira bwino. Reagent iyi ili ndi ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer ndi stabilizer. Amagwiritsidwa ntchito molumikizana ndi lysis buffer amatha kuzindikira mosavuta zitsanzo, ndipo ali ndi mawonekedwe a chidwi chachikulu, kulimba kwamphamvu, ndi kukhazikika kwabwino.

    D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.

  • Zebra Fish Direct PCR Kit-UNG

    Mbidzi Nsomba Direct PCR Kit-UNG

    Chida ichi chimagwiritsa ntchito njira yapadera yolumikizira lysis kutulutsa mwachangu genomic kuchokera ku zebrafish ndi ziwombankhanga zina zamadzi amchere, zipsepse za mchira kapena zitsanzo za mazira a nsomba pakuyankha kwa PCR, kotero ndizoyenera makamaka kuyesa kwakukulu kwa majini. Njira yotulutsa genomic DNA kuchokera ku lysis buffer imamalizidwa mkati mwa 10-30 mphindi pa 65 ° C. Palibenso njira zina monga kuchotsa mapuloteni ndi RNA, ndipo DNA yomwe ingatulutsidwe itha kugwiritsidwa ntchito ngati template ya PCR reaction.

    2 × PCR Easy TM Mix (UNG) imagwiritsa ntchito DUTP m'malo mwa dTTP pamaziko a 2 × PCR Easy TM Mix, ndipo imawonjezera enzyme ya UNG (Uracil-N-glycosylase) yomwe imatha kutsitsa template yomwe ili ndi DUTP nthawi yomweyo. Asanachitike PCR, puloteni ya UNG imagwiritsidwa ntchito kunyoza zomwe PCR imakhala ndi uracil. Enzyme ya UNG sikhala ndi zotsatirapo pazomwe sizikhala ndi uracil, potero zimatsimikizira kulondola ndi kulondola kwa kukulitsa ndikuletsa kuthekera kwa kuyesedwa kwamitundu yayikulu. Kuwonongeka kwa zinthu za PCR kunachitika.

    D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.

  • Plant leaf Direct PCR Plus kit

    Bzalani tsamba la Direct PCR Plus

    Chogulitsachi chimagwiritsa ntchito makina apadera a lysis pofunafuna masamba obzala. Lysate itha kugwiritsidwa ntchito ngati template popanda kuyeretsa. Pambuyo powonjezera zoyambira, PCR Mix ya chida ichi itha kugwiritsidwa ntchito kukulitsa.

  • Plant Seed Direct PCR Kit I/II

    Bzalani Mbewu Mwachindunji PCR Kit I / II

    Chida ichi chimagwiritsa ntchito njira yapadera ya lysis pofesa mbewu. Lysate itha kugwiritsidwa ntchito ngati template popanda kuyeretsa. Pambuyo powonjezera zoyambira, PCR Mix ya chida ichi itha kugwiritsidwa ntchito kukulitsa.

  • Plant leaf Direct PCR plus kit-UNG

    Bzalani tsamba Direct PCR kuphatikiza zida-UNG

    Kutengera chida cha Plant Leaf Direct PCR Plus, dUTP imagwiritsidwa ntchito m'malo mwa dTTP, ndipo enzyme ya UNG yomwe imatha kutsitsa template yomwe ili ndi dUTP imawonjezeredwa. Mwanjira imeneyi, kutsimikizika ndi kulondola kwa kukulitsa kumatsimikiziridwa, ndipo vuto la kuipitsidwa kwa mankhwala a PCR lomwe lingachitike pakuyesa kwamitundu yayikulu limalephereka.

  • Plant leaf Direct PCR kit-UNG

    Bzalani tsamba Direct PCR kit-UNG

    Kutengera chida cha Plant Leaf Direct PCR, dUTP imagwiritsidwa ntchito m'malo mwa dTTP, ndipo enzyme ya UNG yomwe imatha kutsitsa template yomwe ili ndi dUTP imawonjezeredwa. Mwanjira imeneyi, kutsimikizika ndi kulondola kwa kukulitsa kumatsimikiziridwa, ndipo vuto la kuipitsidwa kwa mankhwala a PCR lomwe lingachitike pakuyesa kwamitundu yayikulu limalephereka.