• facebook
  • linkedin
  • youtube
tsamba_banner

Lnc-RT Heroᵀᴹ I(With gDNase)(Super Premix for first-strand cDNA synthesis from lncRNA)

Kufotokozera Kwa Zida:

Super Premix for first-strand cDNA synthesis from lncRNA

Cat.No.RTL-09098/09099

Makina osindikizira ofulumira komanso omvera kwambiri kuti apange cDNA yoyamba kuchokera ku lncRNA.

Chotsani gDNA mu mphindi ziwiri.

Mphindi 15 kuti amalize kaphatikizidwe ka chingwe choyamba cDNA

High-sensitivity reverse transcription system

Kukhazikika kwamafuta ambiri, kutentha kwabwino kwambiri ndi 42 ℃, ndipo kumakhalabe ndi kalembedwe kabwino ka 50 ℃.


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

FAQ

KOWANI ZAMBIRI

Zofotokozera

25×20μl ndi, 50×20μl rxns

Lnc-RT Hero TM I (Ndi gDNase) ndi reverse transcript system yopangidwa mwapadera kuti lncRNA ichotse msanga kuipitsidwa kwa ma genomic DNA.5 × gDNase Mix ikhoza kuchotsa mwamsanga genome yotsala mu RNA pa 42 ° C kwa mphindi 2, popewa kusokoneza kwa genome pa zotsatira za qPCR.

5 × L-RT Hero TM Mix ili ndi Foregene LncRNA Reverse Transcriptase yopangidwa mwapadera ndi Foregene, yomwe ndi mtundu watsopano wa reverse transcriptase makamaka kwa lncRNA ndi ma templates ena aatali a RNA, omwe ali ndi mgwirizano wamphamvu wa RNA komanso kujambula bwino kwambiri.Dongosolo lokhathamiritsa limapangitsa kuti zolembera zosinthika zikhale mwachangu ndipo zimatha kulemba ma tempulo a RNA mosavuta okhala ndi GC yayikulu komanso mawonekedwe achiwiri ovuta.Chingwe choyamba cha cDNA kaphatikizidwe amatha kumaliza pa 42 ° C mu mphindi 15.

Zida zamagulu

5× gDNase Mix
5× L-RT Hero TM Mix
RNase-Free ddH2O
Malangizo

Mbali & ubwino

Kutha kothandiza kuchotsa gDNA, yomwe imatha kuchotsa gDNA mu template mkati mwa mphindi ziwiri.

■ Ikhoza kusinthiratu kulembedwa kwa lncRNA.Pamene katundu wa reverse transcript akutsatiridwa ndi qPCR, mtengo wa Ct umakhala wotsikirapo kuposa wamakina osinthika wamba.

■ Makina osindikizira a reverse, zimangotenga mphindi 15 kumaliza kaphatikizidwe ka chingwe choyamba cha cDNA.

■ Ma tempulo ovuta: ma tempulo okhala ndi GC apamwamba komanso mawonekedwe achiwiri ovuta amathanso kusinthidwa ndikuchita bwino kwambiri.

■ High-sensitivity reverse transcription system, pg-level templates ingathenso kupeza cDNA yapamwamba kwambiri.

■ The reverse transcript system imakhala ndi kukhazikika kwamafuta ambiri, kutentha kwabwino kwambiri ndi 42, ndipo ikadali ndi ntchito yabwino yosinthira ku 50.

Kit application

lncRNA wachibale kuchulukana kusanthula.

lncRNA mtheradi wochulukirachulukira.

Imatha kusanthula mwachangu komanso molondola kuti ifufuze RNA monga kachilombo ka RNA.

Sinthani kumasulira kwa ma tempulo a RNA okhala ndi GC yayikulu kapena mawonekedwe achiwiri ovuta.

Kuyenda kwa ntchito

Lnc-RT HeroTM I(Ndi gDNase)(Super Premix for first-strand cDNA synthesis from lncRNA)

Kusungirako ndi moyo wa alumali

Chidacho chiyenera kusungidwa pa -20°C. Sungani mankhwalawa mufiriji yotentha nthawi zonse pa -20°C atangolandira.Ngati zosungirako zili zoyenera, mankhwalawa sangawononge ntchito iliyonse pazaka zovomerezeka za 1.


  • Zam'mbuyo:
  • Ena:

  • Vuto Kusanthula Buku

    The following is an analysis of the problems that may be encountered in the use of Lnc-RT HeroTM I (With gDNase) series kits, hoping to be helpful to your experiments. In addition, we have dedicated technical support to help you with other experimental or technical problems beyond the operating instructions and questions. If you have any needs, please contact us: 028-83361257 or E-mail: Tech@foregene.com.

     

    Non-kukulitsa kwapadera

    1. Kupanga koyambirira kopanda nzeru

    Malangizo: Pangani zoyambira molingana ndi mfundo zoyambira.

    2. Zotsalira za ma genome.

    Malangizo: Onetsetsani kuti sitepe yoyamba ya kutentha kwa gDNA kuchotsa ndi 42 ° C, ndipo nthawi yochitira imatha kupitilira 5min.

     

    Palibe chizindikiro chokulitsa ndi RT-qPCR

    1. RNA yawonongeka.

    Malangizo: Zopangira RNA ziyenera kukhala zatsopano momwe zingathere, komanso RNA yapamwamba komanso yoyera kwambiri iyenera kugwiritsidwa ntchito.

    2. RNA ili ndi zoletsa.

    Malangizo: Reverse transcription inhibitors nthawi zambiri amaphatikizapo SDS, mchere wa guanidine, EDTA, ndi zina zotero. Ndi bwino kutsuka madzi a RNA ndi 70% ethanol kuchotsa zoletsa.

    3. Nkhani zoyambira kupanga.

    Malangizo: Molingana ndi mfundo yoyambira, panganinso zoyambira kuti ziwunikenso.

     

    Gulu lomwe mukufuna likuwoneka muzowongolera zopanda kanthu

    1. Kudetsedwa kwa zida zogwirira ntchito kapena ma reagents.

    Chidziwitso: Ma reagents onse kapena zida zoyeserera ziyenera kukhala autoclaved.Samalani komanso mofatsa pogwira kuti ma DNA asamalowe mu pipette kapena kutayikira mu chubu cha centrifuge.

    2. Kuipitsidwa kunachitika panthawi yokonzekera dongosolo la PCR.

    Langizo: Samalani mosamala pogwira, monga: kuvala magolovesi a latex, kugwiritsa ntchito nsonga zosefera.Gwiritsani Ntchito Real Time PCR Mix munjira yotsimikizira kuipitsidwa.

    3. Zoyambira ndizowonongeka.

    Malingaliro: Gwiritsani ntchito electrophoresis ya SDS-PAGE kuti muwone ngati zoyambira zawonongeka, ndikusintha ndi zoyambira zatsopano zoyesera kuzindikira za fluorescence.

    Buku Lachidziwitso:

    Lnc-RT HeroTM I(Ndi gDNase)(Super Premix for first-strand cDNA synthesis from lncRNA) Instruction Manual

     

    Lembani uthenga wanu apa ndikutumiza kwa ife