China Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent(Mouse Tail)(for Genotyping) Wopanga ndi Wopereka |Foregene

Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent (Mchira wa Mbewa) (ya Genotyping)

Kufotokozera Kwa Zida:

Mchira wa mbewa wa lysate umagwiritsidwa ntchito mwachindunji ngati template yakukulitsa kwa PCR popanda kutulutsa kosiyana kwa DNA, komwe kumakhala kofulumira komanso kosavuta.Adawonjezera UNG anti-pollution system kuti athetse zolakwika.

◮Palibe kuyeretsa kwa DNA kotenga nthawi komanso kokwera mtengo komwe kumafunikira.

◮Zofuna zachitsanzo ndizochepa, ingotenga mbeu imodzi.

◮Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo ntchitoyo ndi yosavuta.

◮Makina okhathamiritsa a PCR amathandizira PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Kufotokozera

Chidachi chimagwiritsa ntchito njira yapadera ya lysis buffer kuti itulutse mwamsanga DNA ya genomic kuchokera ku michira ya mbewa, makutu, minofu ndi zitsanzo zina zamtundu wa PCR, kotero ndizoyenera kwambiri kuyesa ma genetic.

Njira yotulutsa DNA ya genomic kuchokera ku lysis buffer imatsirizidwa mkati mwa mphindi 10-30 pa 65 ° C.Palibe njira zina monga kuchotsedwa kwa mapuloteni ndi RNA zomwe zimafunikira, ndipo DNA yotulutsidwa ingagwiritsidwe ntchito ngati template ya PCR reaction.

2 × M1-PCR Yosavuta^TMMix (UNG) imagwiritsa ntchito dUTP m'malo mwa dTTP pamaziko a 2 × M1-PCR Easy^TMSakanizani (UNG), ndikuwonjezera UNG enzyme (Uracil-N-glycosylase) yomwe ingawononge template yomwe ili ndi dUTP nthawi yomweyo.PCR isanachitike, puloteni ya UNG imagwiritsidwa ntchito kusokoneza mankhwala a PCR okhala ndi uracil.Enzyme ya UNG sidzakhala ndi zotsatirapo pa template yomwe ilibe uracil, potero imatsimikizira kutsimikizika ndi kulondola kwa kukulitsa ndikuletsa kuthekera kwa kuyesa kwakukulu kwa majini.Kuyipitsidwa kwa zinthu za PCR kunachitika.

D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene kuti achite mwachindunji PCR.D-Taq DNA polymerase ili ndi kulolerana kwamphamvu kwa mitundu yosiyanasiyana ya PCR reaction inhibitors, ndipo imatha kukulitsa bwino kuchuluka kwa DNA m'machitidwe osiyanasiyana ovuta kuchita, ndipo liwiro lokulitsa limatha kufikira 2Kb/mphindi, lomwe ndiloyenera kwambiri kuchitapo kanthu kwa Direct PCR.

Zofotokozera

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Zida zamagulu

Gawo I	Buffer MP
	Foregene Protease Plus
	6 × DNA Loading Buffer
Gawo II	2 × M1 PCR Yosavuta^TMMix(UNG)
Malangizo

Mbali & ubwino

■Palibe kuyeretsa kwa DNA kotenga nthawi komanso kokwera mtengo komwe kumafunikira.

■ Zofuna zachitsanzo ndizochepa, momwe minofu ya mbewa ya 5mg kapena 2-5mm mchira wa mbewa ingayesedwe.

■ Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo opaleshoniyo ndi yosavuta.

■ The wokometsedwa PCR dongosolo zimapangitsa PCR kukhala apamwamba enieni ndi kupirira mwamphamvu PCR reaction inhibitors.

■ Pulogalamu ya PCR yolimbana ndi kuipitsidwa 2×M1-PCR Yosavuta^TMMix (UNG), yomwe imathetsa bwino kuipitsidwa ndi zinthu za PCR ndikuwonetsetsa kutsimikizika ndi kulondola kwa kukulitsa.

Zosintha za Kit

Kuchuluka kwa ntchito: mchira wa mbewa, khutu la mbewa, minofu ndi minofu ina.

DNA yotulutsidwa kuchokera ku lysis yachitsanzo: imagwiritsidwa ntchito ngati template ya PCR.

Zidazi zitha kugwiritsidwa ntchito pazifukwa izi: kuzindikira transgenosis, genotyping ya nyama, ndi zina.

Kuyenda kwa ntchito

Kusungirako ndi moyo wa alumali

Gawo I la zida izi liyenera kusungidwa pa 2-8 ℃.

The reagent Buffer AL ikhoza kusungidwa kwa miyezi 12 pansi pauma;imatha kusungidwa pa 2-8 ℃ kuti isungidwe nthawi yayitali.

Reagent Foregene Protease ili ndi njira yapadera.Kuti muwonetsetse kuti ikugwira ntchito komanso kukhazikika, chonde sungani pa 4 ℃ kwa miyezi 12.

Reagent 6 × DNA Loading Buffer ikhoza kusungidwa ku 4 ℃ kapena -20 ℃ kwa nthawi yayitali.

Gawo II la zida izi ziyenera kusungidwa pa -20 ℃.

Reagent 2 × M1 PCR EasyTM Mix (UNG), ngati imagwiritsidwa ntchito pafupipafupi, imathanso kusungidwa pa 4 ℃ kuti isungidwe kwakanthawi kochepa (kugwiritsa ntchito mkati mwa masiku 10).

Zam'mbuyo: Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent (Mchira wa Mbewa) (ya Genotyping)

Ena: Mouse Tail Direct PCR Kit Direct PCR Lysis Reagent (Mchira wa Mbewa) (ya Genotyping)

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