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Mbewa Mchira Direct PCR Kit
Chida ichi chimagwiritsa ntchito njira yapadera ya lysis kuti imasule mwachangu ma genomic a DNA kuchokera kumiyendo ya mbewa, makutu, minofu ndi mitundu ina ya minyewa yamachitidwe a PCR, chifukwa chake ndioyenera makamaka kuyesa kwakukulu kwa majini.
Njira yotulutsa genomic DNA kuchokera ku lysis buffer imamalizidwa mkati mwa 10-30 mphindi pa 65 ° C. Palibenso njira zina monga kuchotsa mapuloteni ndi RNA, ndipo DNA yomwe ingatulutsidwe itha kugwiritsidwa ntchito ngati template ya PCR reaction.
2 × M1-PCR Easy TM Mix ili ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo itha kugwiritsa ntchito lysate yachitsanzo kuti iyesedwe ngati template yolimbikitsira bwino. Reagent iyi ili ndi Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer ndi stabilizer.
D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene pakuyankha kwa PCR. D-Taq DNA polymerase imakhala ndi kulolerana kwamphamvu kwa ma PCR reaction inhibitors, ndipo imatha kukulitsa kuchuluka kwa DNA m'njira zosiyanasiyana, ndipo liwiro lokulitsa limatha kufikira 2Kb / min, yomwe ili yoyenera makamaka kwa Direct PCR reaction.
