Mouse Tail DNA Mini Kit Genomic DNA Extraction Kits kuchokera ku Mouse Tail
Kufotokozera Kwa Zida:
Zida zothamanga kwambiri padziko lonse lapansi zochotsa DNA ya mbewa ya mbewa, yomwe imatha kutulutsa DNA yapamwamba kwambiri mumchira wa mbewa pasanathe ola limodzi.
◮Palibe kuipitsidwa kwa RNase:DNA-Only Column yoperekedwa ndi zidayi imapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda kuwonjezera RNase panthawi yoyesera, kuletsa labotale kuti isaipitsidwe ndi RNase yachilendo.
◮Liwiro:Foregene Protease imakhala ndi zochita zambiri kuposa ma protease ofanana, ndipo imagaya zitsanzo za minofu mwachangu;ntchitoyo ndi yosavuta, ndipo ntchito yochotsa DNA ya genomic imatha kutha mkati mwa mphindi 20-80.
◮Zabwino:The centrifugation ikuchitika kutentha firiji, ndipo palibe chifukwa 4 ° C otsika kutentha centrifugation kapena Mowa mpweya wa DNA.
◮Chitetezo:Palibe organic reagent m'zigawo zofunika.
◮Mapangidwe apamwamba:DNA yotulutsidwa ya genomic ili ndi zidutswa zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira zosiyanasiyana.
◮Microelution System:Itha kuwonjezera kuchuluka kwa ma DNA amtundu, omwe ndi osavuta kuzindikira kapena kuyesa kuyesa.
Kufotokozera
Chifukwa cha kapangidwe ndi katundu wa mbewa mchira palokha, m'zigawo za mbewa mchira genomic DNA wakhala akuvutitsa ofufuza: mmodzi ndi kuti processing nthawi ya mbewa mchira ndi yaitali ndipo DNA genomic mosavuta amadetsedwa, wina ndi zovuta kusamalira ndi chiyero chotengedwa DNA genomic ndi otsika.
Chidachi chimagwiritsa ntchito DNA-Only Column yomwe imatha kumangirira DNA, Foregene Protease yatsopano komanso makina apadera achitetezo, omwe amatha kukumba zitsanzo za mchira wa mbewa mkati mwa mphindi 45, potero kuchepetsa kuwonongeka kwa DNA ya genomic ndikufupikitsa nthawi yokonza zitsanzo.Chidacho chimatha kutulutsa 10-20μg ya DNA yapamwamba kwambiri, yoyera kwambiri ya genomic kuchokera ku 0.5-1cm michira yachinyamata kapena mbewa yachikulire mkati mwa 60-90min.
DNA-yokha silica gel matrix zakuthupi zomwe zimagwiritsidwa ntchito pozungulira ndi chinthu chatsopano cha kampani, chomwe chimatha kutsatsa DNA moyenera komanso mwapadera.Kuchuluka kwa adsorption kwa DNA ndi 80μg.Dongosolo lapadera la buffer ndi elution limatha kukulitsa kuchotsedwa kwa RNA, mapuloteni odetsedwa, ayoni ndi zinthu zina zakuthupi mu cell.Chidutswa cha DNA chotengedwa ndi chachikulu, choyera kwambiri, chokhazikika komanso chodalirika, ndipo kukula kwa chidutswa cha DNA ndi chokhazikika pafupifupi 23kb.
Chidacho chingagwiritsidwenso ntchito kuchotsa minyewa ina ya mbewa kunja kwa mchira wa mbewa, kuphatikizapo makutu a mbewa, minofu ya mbewa, chiwindi cha mbewa ndi minyewa ina, kotero kuti chida chimodzi chitha kugwiritsidwa ntchito pazinthu zingapo ndikukwaniritsa zosowa za ogwiritsa ntchito pazoyeserera zosiyanasiyana.
Zofotokozera
50 Preps, 100 Preps, 250 Preps
Zida zamagulu
| Mtengo wa TL1 |
| Bafa TL2* |
| PW * |
| Mtengo WB |
| Mtengo wa EB |
| Foregene Protease Plus |
| Nambala ya DNA Yokha |
| Malangizo |
Mbali & ubwino
-Palibe kuipitsidwa kwa RNase: Gawo la DNA-Only loperekedwa ndi zidali limapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda kuwonjezera RNase panthawi yoyesera, kupewa labotale kuti isaipitsidwe ndi RNase yachilendo.
-Liwiro lofulumira: Foregene Protease Plus ili ndi zochita zambiri kuposa ma protease ofanana, ndipo imagaya zitsanzo za minofu mwachangu;ntchitoyo ndi yosavuta, ndipo ntchito yochotsa ma genomic DNA imatha kutha mkati mwa mphindi 40.
- Yabwino: The centrifugation ikuchitika kutentha firiji, ndipo palibe chifukwa 4 ° C otsika kutentha centrifugation kapena Mowa mpweya wa DNA.
-Safety: Palibe organic reagent m'zigawo zofunika.
-Zapamwamba kwambiri: DNA yotulutsidwa ya genomic ili ndi zidutswa zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira za mayesero osiyanasiyana.
Kit application
Ndiwoyenera kuchotsa ndi kuyeretsa DNA ya genomic kuchokera ku michira yatsopano kapena yowundana ndi mbewa (yomwe imagwiritsidwanso ntchito kuyeretsa mbewa kapena minofu ya makoswe ndi DNA ya khutu la mbewa).
Kayendedwe kantchito
Kusungirako ndi moyo wa alumali
-Chidachi chikhoza kusungidwa kwa miyezi 12 pansi pa nyengo youma kutentha (15-25 ° C);Ngati ikufunika kusungidwa kwa nthawi yayitali, imatha kusungidwa pa 2-8 ° C.
Zindikirani: Ngati atasungidwa kutentha kochepa, yankho limakhala lamvula.Musanagwiritse ntchito, onetsetsani kuti mwayika yankholo mu zida pa firiji kwakanthawi.Ngati n'koyenera, preheat mu madzi osamba 37 ° C kwa mphindi 10 kuti asungunuke mpweya, ndikusakaniza musanagwiritse ntchito.
-Foregene Protease Plus yankho lili ndi ndondomeko yapadera, imagwira ntchito ikasungidwa kutentha kwa nthawi yaitali (miyezi itatu);kusungidwa pa 4 ° C, ntchitoyo ndi kukhazikika kudzakhala bwino, choncho ndi bwino kusunga pa 4 ° C, kumbukirani kuti musamasunge -20 ° C.
