China Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Mchira wa Mbewa)(wa Genotyping) Wopanga ndi Wopereka |Foregene

Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent (Mchira wa Mbewa) (ya Genotyping)

Kufotokozera Kwa Zida:

Mwachindunji gwiritsani ntchito mbewa mchira wa lysate ngati template yakukulitsa kwa PCR, popanda kutulutsa kosiyana kwa DNA, kufulumira komanso kukhudzika kwambiri.

◮Palibe kuyeretsa kwa DNA kotenga nthawi komanso kokwera mtengo komwe kumafunikira.

◮Zofuna zachitsanzo ndizochepa, ingotenga mbeu imodzi.

◮Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo ntchitoyo ndi yosavuta.

◮Makina okhathamiritsa a PCR amathandizira PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Zofotokozera

Chidachi chimagwiritsa ntchito njira yapadera ya lysis buffer kuti itulutse mwachangu ma genomic DNA kuchokera ku mchira wa mbewa ndi zitsanzo za khutu la mbewa za PCR reaction, kotero ndizoyenera kuyesa kwakukulu kwa majini.

Njira yotulutsa DNA ya genomic kuchokera ku lysis buffer yathamkati mwa mphindi 10-30kukutentha kwa chipinda (20-25).°C).Palibe kutentha komwe kumafunikira, ndipo njira zina monga mapuloteni ndi kuchotsa RNA sizofunika.Ma DNA omwe atulutsidwa atha kugwiritsidwa ntchito ngati Template ya PCR reaction.

2 × M-PCR Yosavuta^TMMix ili ndi kulolerana kwamphamvu kwa PCR reaction inhibitors, ndipo imatha kugwiritsa ntchito lysate yachitsanzo kuti iyesedwe ngati template yokulitsa bwino komanso yeniyeni.Reagent iyi ili ndi Foregene D-Taq DNAPolymerase, dNTPs, MgCl₂, zotchingira zochita, PCR optimizer ndi stabilizer.

D-Taq DNA polymerase ndi DNA polymerase yopangidwa mwapadera ndi Foregene kuti achite mwachindunji PCR.D-Taq DNA polymerase ili ndi kulolerana kwamphamvu kwa mitundu yosiyanasiyana ya PCR reaction inhibitors, ndipo imatha kukulitsa bwino kuchuluka kwa DNA m'machitidwe osiyanasiyana ovuta kuchita, ndipo liwiro lokulitsa limatha kufikira 2Kb/mphindi, lomwe ndiloyenera kwambiri kuchitapo kanthu kwa Direct PCR.

Zofotokozera

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Zida zamagulu

Gawo I	Mtengo NP
	Foregene Protease Plus I
	6 × DNA Loading Buffer
Gawo II	2 × M PCR EasyTM Mix
Malangizo

Mbali & ubwino

■Palibe kuyeretsa kwa DNA kotenga nthawi komanso kokwera mtengo komwe kumafunikira.

■ Zofuna zachitsanzo ndizochepa, ingotengani michira ya mbewa (kapena makutu a mbewa okhala ndi mainchesi 2-5mm) pamwamba pa 2mm.

■ Yosavuta kugwiritsa ntchito, imatha kuyikidwa popanda kutentha kuti ipangitse protease, ndipo chopangidwa ndi lysed chikhoza kuchitidwa mwachindunji ndi PCR.

■ Foregene Mouse Tail Genomic DNA Extraction Kit (DE-05211) ingagwiritsidwe ntchito kuchotsa DNA ya genomic kuchokera ku mchira wa makoswe.

■ Makina a PCR okometsedwa amapangitsa PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.

Zosintha za Kit

Ntchito: Mchira wa mbewa, makutu a mbewa, minofu ndi minofu ina.

DNA yotulutsidwa kuchokera ku lysis yachitsanzo: imagwiritsidwa ntchito ngati template ya PCR.

Zidazi zitha kugwiritsidwa ntchito pazifukwa izi: kuzindikira transgenosis, genotyping ya nyama, ndi zina.

Kuyenda kwa ntchito

Kusungirako ndi moyo wa alumali

Gawo I la zida izi liyenera kusungidwa pa 2-8 ℃.

The reagent Buffer FP ikhoza kusungidwa kwa miyezi 12 pansi pauma;imatha kusungidwa pa 2-8 ℃ kuti isungidwe nthawi yayitali.

Reagent Foregene Protease ili ndi njira yapadera.Kuti muwonetsetse kuti ikugwira ntchito komanso kukhazikika, chonde sungani pa 4 ℃ kwa miyezi 12.

Reagent 6 × DNA Loading Buffer ikhoza kusungidwa ku 4 ℃ kapena -20 ℃ kwa nthawi yayitali.

Gawo II la zida izi ziyenera kusungidwa pa -20 ℃.

Reagent 2 × PCR Easy TM Mix, ngati imagwiritsidwa ntchito kawirikawiri, imathanso kusungidwa pa 4 ℃ kuti isungidwe kwakanthawi kochepa (kugwiritsa ntchito mkati mwa masiku 10).

Zam'mbuyo: Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(mbidzi)

Ena: Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent (Mchira wa Mbewa) (ya Genotyping)

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