Kumayambiriro kwa mliriwu, chifukwa chakukula mwachangu, kuzindikira mwachangu kwa odwala omwe akuwakayikira ndiye chinsinsi chopewera COVID-19.Ma reagents ena ovomerezeka a nucleic acid amakhala ndi nthawi yayitali yachitukuko, ndipo pali zovuta monga kutsimikizira magwiridwe antchito mwachangu, kukhathamiritsa kwa reagent, komanso kusiyana kwakukulu pakati pamagulu;Mavuto a ma laboratories osiyanasiyana azachipatala m'magawo osiyanasiyana a nucleic acid kuzindikira atha kukhudzanso kulondola kwa zotsatira za nucleic acid.Nkhaniyi ifotokoza za maulalo ndi mfundo zazikuluzikulu pakuzindikirika kwaposachedwa kwa SARS-CoV-2 nucleic acid, ndikuwunikanso zovuta zabodza komanso kuwunikanso kolondola kwa labotale ya nucleic acid kuzindikira komanso kusagwirizana kwachipatala.

Mfundo za SARS-CoV-2 nucleic acid kuzindikira

SARS-CoV-2 ndi kachilombo ka RNA komwe kamakhala ndi ma genome pafupifupi 29 kb, okhala ndi majini 10, omwe amatha kuphatikiza mapuloteni 10.Mavairasi amapangidwa ndi RNA ndi mapuloteni, ndipo wosanjikiza wakunja ndi wokutira wakunja wopangidwa ndi lipids ndi glycoproteins.M'kati mwake, puloteni yotchedwa capsid imakutira RNA mmenemo, motero imateteza RNA (P1) yomwe imatha kuwonongeka mosavuta.

P1 Kapangidwe ka SARS-COV-2

Ma virus amalowa m'maselo kudzera m'maselo apadera a cell kuti ayambitse matenda, ndikugwiritsa ntchito ma cell omwe akukhala nawo kuti abwereze.

Mfundo yozindikiritsa ma virus nucleic acid ndikuvumbulutsa kachilombo ka RNA kudzera mu cell lysate, kenako kugwiritsa ntchito nthawi yeniyeni ya fulorosenti reverse transcription-polymerase chain reaction (RT-PCR) kuti azindikire.

Chinsinsi cha mfundo yodziwikiratu ndikugwiritsa ntchito zoyambira ndi ma probes kuti mukwaniritse "kufananitsa" kwa ma nucleic acid, ndiye kuti, kupeza ma nucleic acid a SARS-CoV-2 omwe ndi osiyana ndi ma virus ena pafupifupi 30,000 maziko (kufanana kwa nucleic acid ndi ma virus ena) "Low" dera), zoyambira ndi ma probe.

Zoyambira ndi ma probes zimagwirizana kwambiri ndi dera la SARS-CoV-2 nucleic acid, ndiye kuti, kutsimikizika kwake ndikwamphamvu kwambiri.Zotsatira zakukwezeredwa kwa fulorosenti ya RT-PCR zenizeni zachitsanzo kuti ziyesedwe zimakhala zabwino, zimatsimikizira kuti SARS-CoV-2 ilipo pachitsanzocho.Onani P2.

P2 Masitepe a SARS-CoV-2 nucleic acid kutsimikiza (nthawi yeniyeni fulorosenti RT-PCR)

Mikhalidwe ndi zofunikira za labotale pakuzindikira kwa SARS-CoV-2 nucleic acid

Ma labotale oyesa ma Nucleic acid ndi omwe ali abwino kwambiri potengera kupanikizika koyipa, ndipo akuyenera kuyang'anitsitsa kuwunikira, kusunga mpweya, ndikuchotsa ma aerosols.Ogwira ntchito yoyesa ma Nucleic acid ayenera kukhala ndi ziyeneretso zofananira, alandire maphunziro oyenera a polymerase chain reaction ndikupambana mayesowo.Laborator iyenera kuyang'aniridwa mosamalitsa, yoyikidwa pamalo ake, ndipo ogwira ntchito osayenera ndi oletsedwa kulowa.Malo aukhondo ayenera kukhala ndi mpweya wabwino ndi kuthira tizilombo.Zinthu zofunikira zimayikidwa m'magawo, zoyera ndi zakuda zimasiyanitsidwa, kusinthidwa munthawi yake, ndikuchotsedwa m'malo mwake.Kupha tizilombo toyambitsa matenda: Mankhwala okhala ndi chlorine ndi njira yayikulu yothetsera madera akuluakulu, ndipo 75% ya mowa imatha kugwiritsidwa ntchito kumadera ang'onoang'ono.Njira yabwino yothanirana ndi ma aerosols ndikutsegula mazenera olowera mpweya wabwino, ndipo kupha tizilombo toyambitsa matenda kungathenso kuchitidwa ndi cheza cha ultraviolet, kusefera, ndi kupha tizilombo toyambitsa matenda.

Maulalo ofunikira ndi magawo a SARS-CoV-2 nucleic acid determination (real-time fluorescent RT-PCR)

Ngakhale ma laboratories nthawi zambiri amasamalira kwambiri "kuzindikira" kwa nucleic acid, kwenikweni, "kutulutsa" kwa nucleic acid ndi imodzi mwamasitepe ofunikira kuti azindikire bwino, zomwe zimagwirizana kwambiri ndi kusonkhanitsa ndi kusunga zitsanzo za virus.

Pakalipano, zitsanzo zogwiritsidwa ntchito kwambiri za kupuma, monga swabs za nasopharyngeal, zimagwiritsa ntchito njira yachiwiri, yomwe ndi njira yothetsera (kusungirako) yokonzedwa yochokera ku nucleic acid m'zigawo ndi njira ya lysis.Kumbali imodzi, njira yotetezera kachilomboka imatha kusokoneza puloteni ya kachilomboka, kutaya ntchito zake komanso kusakhalanso ndi matenda, ndikuwongolera chitetezo chamayendedwe ndi kuzindikira;Kumbali ina, imatha kusokoneza kachilomboka mwachindunji kuti itulutse nucleic acid, kuchotsa puloteni yowola ya nucleic acid, ndikuletsa kachilomboka.RNA imachotsedwa.

Njira yothetsera ma virus yokonzedwa pamaziko a nucleic acid extraction lysis solution.Zigawo zazikuluzikulu ndi mchere woyenerera, ethylenediaminetetraacetic acid chelating agent, mchere wa guanidine (guanidine isothiocyanate, guanidine hydrochloride, etc.), anionic surfactant (dodecane) sodium sulfate), cationic surfactant (tetradecyl methyloxalate, hydrochloride, hydrochloride, hydrochloride, hydrochloride, tetradecyl trioxyl, hydrochloride), proteinase K ndi zigawo zina zingapo kapena zambiri.Pakalipano, pali mitundu yambiri ya zida za nucleic acid m'zigawo, ndipo ma nucleic acid m'zigawo zosiyanasiyana ndi ma reagents oyeretsa amagwiritsidwa ntchito.Ngakhale ngati nucleic acid m'zigawo ndi kuyeretsa reagent ntchito, njira m'zigawo za zida aliyense ndi osiyana.

Pakadali pano, zida zozindikirira ma nucleic acid zomwe zavomerezedwa ndi National Medical Products Administration zimasankhidwa kutengera ma jini a ORF1ab, E ndi N mu genome ya SARS-CoV-2.Mfundo zodziwikiratu zazinthu zosiyanasiyana ndizofanana, koma zoyambira ndi kapangidwe kake ndizosiyana.Pali magawo a chandamale chimodzi (ORF1ab), magawo a chandamale apawiri (ORF1ab, N kapena E), ndi magawo atatu (ORF1ab, N ndi E).Kusiyana pakati pa kuzindikira ndi kutanthauzira, nucleic acid m'zigawo ndi nthawi yeniyeni ya fluorescent RT-PCR reaction system iyenera kutchula malangizo oyenerera a zida, ndipo tikulimbikitsidwa kuti ogwiritsa ntchito atsatire mosamalitsa njira yomasulira yomwe ili mu malangizo a zida kuti amasulire.Madera wamba, zoyambira ndi ma probe amatsatiridwa ndi fulorosenti RT-PCR yeniyeni akuwonetsedwa mu P3.

P3 Malo a SARS-CoV-2 amplicon chandamale pa genome ndi mndandanda wa zoyambira ndi zofufuza.

Kutanthauzira kwa zotsatira za SARS-CoV-2 nucleic acid determination (Rku-Tfulorosenti RT-PCR)

"Pneumonia Prevention and Control Plan for SARS-CoV-2 Infection (Second Edition)" kwa nthawi yoyamba idafotokoza njira zowonera zotsatira zakukula kwa jini imodzi:

1. Palibe Ct kapena Ct≥40 ndi zoipa;

2. Ct<37 ndi zabwino;

3. Mtengo wa Ct wa 37-40 ndi dera la imvi.Ndi bwino kubwereza kuyesera.Ngati zotsatira za kubwereza Ct <40 ndi mapindikidwe okulitsa ali ndi nsonga zoonekeratu, chitsanzocho chimayesedwa ngati chabwino, apo ayi ndi cholakwika.

Buku lachitatu la bukhuli ndi buku lachinayi la bukhuli linapitirizabe zomwe zili pamwambazi.Komabe, chifukwa cha zolinga zosiyanasiyana zomwe zimagwiritsidwa ntchito muzogulitsa zamalonda, kope la 3 lomwe tatchulalo la bukhuli silinapereke njira zowonetsera kuphatikizika kwa zolinga, kutsindika Malangizo operekedwa ndi wopanga adzapambana.Kuyambira pa kope lachisanu la malangizowa, zolinga ziwiri zafotokozedwa, makamaka ziweruzo za cholinga chimodzi chomwe chiri chovuta kuweruza.Ndiye kuti, ngati labotale ikufuna kutsimikizira kuti mlandu ndi wabwino pakuzindikira kwa SARS-CoV-2 nucleic acid, zotsatirazi ziyenera kukwaniritsidwa 1 mwa mikhalidwe iwiri:

(1) Zolinga ziwiri za SARS-CoV-2 (ORF1ab, N) m'chitsanzo chomwecho zimayesedwa ndi fulorosenti RT-PCR yeniyeni.Ngati cholinga chimodzi chili chabwino, kuyesanso ndi kuyesanso ndikofunikira.Ngati zotsatira zoyezetsa zili Ngati cholinga chimodzi chikadali chabwino, chimayesedwa kuti chili ndi HIV.

(2) Zitsanzo ziwiri za nthawi yeniyeni ya fulorosenti RT-PCR inasonyeza chandamale chimodzi chabwino panthawi imodzi kapena zitsanzo ziwiri za mtundu womwewo zinasonyeza zotsatira zabwino zoyesa, zomwe zingayesedwe kuti ndizo zabwino.Komabe, malangizowo akutsindikanso kuti zotsatira zoyipa zakuyesa kwa nucleic acid sizingaphatikizepo matenda a SARS-CoV-2.Zinthu zomwe zingayambitse zolakwika zabodza ziyenera kuchotsedwa, kuphatikizapo khalidwe loipa lachitsanzo (zitsanzo za kupuma kuchokera ku oropharynx ndi mbali zina), kusonkhanitsa zitsanzo mofulumira kwambiri kapena mochedwa kwambiri, Zitsanzo sizinasungidwe, kunyamulidwa, ndi kukonzedwa moyenera, ndipo teknoloji yokha inali ndi mavuto (kusiyana kwa mavairasi, PCR inhibition), ndi zina zotero.

Zomwe zimayambitsa zoyipa zabodza pakuzindikirika kwa SARS-CoV-2

Lingaliro la "bodza labodza" pakuyesa kwa nucleic acid lomwe likukhudza pano, nthawi zambiri limatanthawuza "zolakwika zabodza" momwe zotsatira za mayeso a nucleic acid sizigwirizana ndi mawonetseredwe azachipatala, ndiye kuti, zizindikiro zachipatala ndi zotsatira zazithunzi zimaganiziridwa kwambiri za COVID-19, koma mayeso a nucleic acid amakhala "oyipa" nthawi zambiri.Clinical Laboratory Center ya National Health Commission idafotokoza za kuyesa kwa SARS-CoV-2 "zabodza".

(1) M’maselo a munthu amene ali ndi kachilomboka muli kachilombo kochuluka.Deta yomwe ilipo ikuwonetsa kuti thupi likakhala ndi kachilomboka, kachilomboka kamalowa m'mphuno ndi pakamwa, kenako ku trachea ndi bronchi, kenako kumafika ku alveoli.The kachilombo munthu adzaona makulitsidwe nthawi, wofatsa zizindikiro, ndiyeno ndondomeko kwambiri zizindikiro, ndi magawo osiyanasiyana a matenda.Ndipo kuchuluka kwa kachilombo komwe kamapezeka m'zigawo zosiyanasiyana za thupi kumakhala kosiyana.

Pankhani ya kuchuluka kwa ma virus amitundu yama cell, alveolar epithelial cell (m'munsi kupuma thirakiti)> airway epithelial cell (pamwamba kupuma thirakiti)> fibroblasts, endothelial cell, ndi macrophages, ndi zina zambiri;kuchokera ku mtundu wa chitsanzo, alveolar lavage fluid (yopambana kwambiri)>kutsokomola sputum>nasopharyngeal swab>oropharyngeal swab>magazi.Kuphatikiza apo, kachilomboka kamathanso kupezeka mu ndowe.Komabe, poganizira kumasuka kwa opaleshoni ndi kuvomereza kwa odwala, chitsanzo chachipatala chomwe chimagwiritsidwa ntchito kwambiri ndi oropharyngeal swab>nasopharyngeal swab>bronchial lavage fluid (opaleshoni yovuta) ndi sputum wakuya (nthawi zambiri chifuwa chouma, chovuta kuchipeza).

Choncho, kuchuluka kwa kachilomboka m'maselo a oropharynx kapena nasopharynx odwala ena ndi ochepa kapena otsika kwambiri.Ngati zitsanzo za oropharynx kapena nasopharynx zimatengedwa kuti ziyesedwe, ma virus nucleic acid sangawonekere.

(2) Palibe ma cell omwe ali ndi ma virus omwe adasonkhanitsidwa panthawi yosonkhanitsa zitsanzo, kapena ma viral nucleic acid sanasungidwe bwino.

[① Malo osonkhanitsira olakwika, mwachitsanzo, posonkhanitsa ma oropharyngeal swabs, kuya kwa kusonkhanitsa sikokwanira, nsonga za nasopharyngeal zosonkhanitsidwa sizimasonkhanitsidwa mozama mumphuno, ndi zina zambiri. Maselo ambiri omwe amasonkhanitsidwa angakhale maselo opanda kachilombo;

②Sampling swabs amagwiritsidwa ntchito molakwika.Mwachitsanzo, ulusi wopangidwa monga PE ulusi, ulusi wa poliyesitala ndi ulusi wa polypropylene amalimbikitsidwa pazinthu zamutu wa swab.Ulusi wachilengedwe monga thonje umagwiritsidwa ntchito pochita ntchito yeniyeni (kutsekemera kwamphamvu kwa mapuloteni komanso kosavuta kutsuka) Ndi ulusi wa nayiloni (mayamwidwe osakwanira amadzi, zomwe zimapangitsa kuti pakhale sampuli yosakwanira);

③Kugwiritsa ntchito molakwika machubu osungira ma virus, monga kugwiritsa ntchito molakwika machubu osungira pulasitiki a polypropylene kapena polyethylene omwe ndi osavuta kuyamwa ma nucleic acid (DNA/RNA), zomwe zimapangitsa kuchepa kwa kuchuluka kwa nucleic acid posungira.M'malo mwake, tikulimbikitsidwa kugwiritsa ntchito pulasitiki ya polyethylene-propylene polima ndi zida zapulasitiki za polypropylene kuti musunge ma virus nucleic acid.]

[① Malo osonkhanitsira olakwika, mwachitsanzo, posonkhanitsa ma oropharyngeal swabs, kuya kwa kusonkhanitsa sikokwanira, nsonga za nasopharyngeal zosonkhanitsidwa sizimasonkhanitsidwa mozama mumphuno, ndi zina zambiri. Maselo ambiri omwe amasonkhanitsidwa angakhale maselo opanda kachilombo;

②Sampling swabs amagwiritsidwa ntchito molakwika.Mwachitsanzo, ulusi wopangidwa monga PE ulusi, ulusi wa poliyesitala ndi ulusi wa polypropylene amalimbikitsidwa pazinthu zamutu wa swab.Ulusi wachilengedwe monga thonje umagwiritsidwa ntchito pochita ntchito yeniyeni (kutsekemera kwamphamvu kwa mapuloteni komanso kosavuta kutsuka) Ndi ulusi wa nayiloni (mayamwidwe osakwanira amadzi, zomwe zimapangitsa kuti pakhale sampuli yosakwanira);

③Kugwiritsa ntchito molakwika machubu osungira ma virus, monga kugwiritsa ntchito molakwika machubu osungira pulasitiki a polypropylene kapena polyethylene omwe ndi osavuta kuyamwa ma nucleic acid (DNA/RNA), zomwe zimapangitsa kuchepa kwa kuchuluka kwa nucleic acid posungira.M'malo mwake, tikulimbikitsidwa kugwiritsa ntchito pulasitiki ya polyethylene-propylene polima ndi zida zapulasitiki za polypropylene kuti musunge ma virus nucleic acid.]

(4) Ntchito ya labotale yachipatala siimakhazikika.Zitsanzo zamayendedwe ndi kusungirako, magwiridwe antchito okhazikika a labotale azachipatala, kutanthauzira kwazotsatira ndi kuwongolera bwino ndizo zinthu zazikuluzikulu zowonetsetsa kulondola komanso kudalirika kwa zotsatira za mayeso.Malinga ndi zotsatira za kuwunika kwakunja komwe kunachitika ndi Clinical Laboratory Center ya National Health Commission pa Marichi 16-24, 2020, mwa ma labotale 844 omwe adalandira zotsatira zolondola, 701 (83.1%) anali oyenerera, ndipo 143 (16.9%) sanali.Oyenerera, miyeso yonse yoyezetsa ma labotale ndiabwino, koma ma labotale osiyanasiyana akadali ndi kusiyana kwa magwiridwe antchito a ogwira ntchito, kuthekera kotanthauzira kwachitsanzo chimodzi, komanso kuwongolera bwino.

Momwe mungachepetsere zabodza za SARS-CoV-2 nucleic acid kuzindikira?

Kuchepetsa zolakwika zabodza pakuzindikira kwa nucleic acid kuyenera kukonzedwa kuchokera kuzinthu zinayi zopanga zolakwika zabodza.

(1)M’maselo a munthu amene ali ndi kachilomboka muli kachilombo kochuluka.Kuchuluka kwa kachilomboka m'madera osiyanasiyana a thupi la omwe akuganiziridwa kuti ali ndi kachilomboka kumakhala kosiyana nthawi zosiyanasiyana.Ngati palibe pharynx, ikhoza kukhala mu bronchial lavage fluid kapena ndowe.Ngati mitundu ingapo ya zitsanzo ingasonkhanitsidwe nthawi imodzi kapena pazigawo zosiyanasiyana za kukula kwa matenda kukayezetsa, Zingathandize kupewa zolakwika zabodza.

(2) Maselo okhala ndi kachilombo amayenera kusonkhanitsidwa panthawi yosonkhanitsa zitsanzo.Vutoli litha kuthetsedwa kwambiri polimbikitsa maphunziro a otolera zitsanzo.

(3) Ma reagents odalirika a IVD.Pochita kafukufuku wokhudzana ndi kuwunika kwa magwiridwe antchito amtundu wamtundu uliwonse, ndikukambirana zovuta zomwe zidalipo, kuzindikira kwa ma reagents kumatha kupititsidwa patsogolo komanso kuzindikira kwa kusanthula kumatha kupitilizidwa.

(4) Ntchito yokhazikika ya ma laboratories azachipatala.Mwa kulimbikitsa maphunziro a ogwira ntchito m'ma laboratory, kupititsa patsogolo kasamalidwe ka khalidwe la ma laboratory mosalekeza, kuonetsetsa magawano oyenera, ndi kupititsa patsogolo luso la ogwira ntchito kuti azindikire, ndizotheka kuchepetsa zolakwika zabodza chifukwa cha ntchito zosayenera za labotale.

Zifukwa zoyezetsanso kuyesa kwa SARS-CoV-2 nucleic acid mwa odwala omwe achira komanso otulutsidwa

"COVID-19 Diagnosis and Treatment Plan (Trial Seventh Edition)" imafotokoza momveka bwino kuti njira imodzi yoti odwala a COVID-19 achire ndikutulutsidwa m'chipatala ndikuti zitsanzo ziwiri zotsatizana za kupuma kwa ma nucleic acid (maola 24 motalikirana), koma ndi ochepa kwambiri.

(1)SARS-CoV-2 ndi kachilombo katsopano.M`pofunika kupitirira kumvetsa ake tizilombo limagwirira, chithunzi chonse cha matenda chifukwa ndi makhalidwe a matenda Inde.Chifukwa chake, kumbali imodzi, ndikofunikira kulimbikitsa kasamalidwe ka odwala omwe atulutsidwa ndikuwunika kwachipatala masiku 14.Chitani zotsatirazi, kuyang'anira zaumoyo ndi chitsogozo cha thanzi kuti mumvetse bwino ndondomeko yonse ya zochitika, chitukuko ndi zotsatira za matendawa.

(2)Wodwala akhoza kutenga kachilomboka kachiwiri.Katswiri wamaphunziro Zhong Nanshan adati: Chifukwa odwala ochiritsidwa ali ndi ma antibodies, SARS-CoV-2 imatha kuthetsedwa ndi ma antibodies akabweranso.Pali zifukwa zambiri, zomwe zitha kukhala zomwe zidayambitsa wodwalayo, kapena zitha kukhala zokhudzana ndi kusintha kwa kachilomboka, kapenanso chifukwa chakuyesa kwa labotale.Ngati ndi kachilombo komweko, kusintha kwa SARS-CoV-2 kumatha kupangitsa kuti anti-antibody yopangidwa ndi wodwala wochirayo kuti ikhale yopanda mphamvu polimbana ndi kachilombo kosinthika.Ngati wodwalayo ali ndi kachilombo kosinthika kachiwiri, kuyesa kwa nucleic acid kungakhalenso ndi HIV.

(3) Ponena za njira zoyezera ma labotale, njira iliyonse yoyesera ili ndi malire ake.Kuzindikira kwa SARS-CoV-2 nucleic acid kumachitika chifukwa cha kusankha kwa jini, kapangidwe ka ma reagents, kukhudzika kwa njira ndi zifukwa zina, zomwe zimapangitsa kuti zida zomwe zilipo zili ndi malire awo otsika.Wodwalayo akalandira chithandizo, kachilomboka mthupi kamachepa.Pamene chiwerengero cha mavairasi mu chitsanzo chomwe chiyenera kuyesedwa chiri pansi pa malire otsika, zotsatira "zoyipa" zidzawonekera.Komabe, izi sizikutanthauza kuti kachilombo kamene kali m'thupi katha.Kachilomboka kakhoza kukhala pambuyo posiya mankhwala.Resurgence”, pitilizani kukopera.Choncho, tikulimbikitsidwa kubwereza kamodzi pa sabata mkati mwa masabata a 2 mpaka 4 mutatha kutulutsa.

(4) Nucleic acid ndi chibadwa cha kachilomboka.Kachilomboka amaphedwa wodwala atalandira mankhwala oletsa tizilombo toyambitsa matenda, koma zidutswa za RNA zotsalazo zimasungidwabe m'thupi la munthu, ndipo sizimatulutsidwa m'thupi.Nthawi zina, m'mikhalidwe ina, imatha kusungidwa kwambiri.Kwa nthawi yayitali, ndipo panthawiyi kuyesa kwa nucleic acid kudzakhala "kanthawi kochepa" kwabwino.Ndi kuwonjezereka kwa nthawi ya kuchira kwa wodwalayo, zidutswa zotsalira za RNA m'thupi zikatha pang'onopang'ono, zotsatira za mayeso a nucleic acid zimatha kukhala zoipa.

(5) Zotsatira za mayeso a nucleic acid za SARS-CoV-2 zimangotsimikizira kukhalapo kapena kusowa kwa viral RNA, ndipo sizingatsimikizire momwe kachilomboka kamagwira komanso ngati kachilomboka kamafalikira.Ndikofunikira kutsimikizira ngati wodwala yemwe adayezetsanso kuti ali ndi nucleic acid adzakhalanso gwero la matenda.Ndikofunikira kuchita chikhalidwe cha ma virus pazitsanzo zamankhwala ndikukulitsa kachilombo ka "moyo" kuti titsimikizire kuti ndi kachilombo.

Chidule

Mwachidule, SARS-CoV-2 nucleic acid kuyesa zolakwika zabodza, zoyesereranso, ndi zina zomwe sizikugwirizana ndi mawonekedwe azachipatala sizingapewedwe kwathunthu.Pakuwunika kwenikweni ndi kuyezetsa, tikulimbikitsidwa kuphatikiza zizindikiro zachipatala, mayeso oyerekeza (CT) ndi kuyesa kwa Laboratory (kuyesa kwa nucleic acid + ma virus-specific antibody test) kumapangitsa kuti azindikire mozama kuti apewe matenda omwe adaphonya komanso kusazindikira.Ngati zotsatira zoyezetsa zikuwoneka kuti sizikugwirizana ndi mawonetseredwe azachipatala, tikulimbikitsidwa kuti tifufuze mozama za ulalo wonse woyeserera (zosonkhanitsa zitsanzo, ma circulation and processing links) kuti asatengere kachilombo ka SARS-CoV-2 kachilombo koyambirira, matenda obwerezabwereza kapena kuphatikiza ndi matenda ena a virus opuma, etc.Ngati zinthu zilola, tikulimbikitsidwa kuti mutenge zitsanzo za sputum kapena alveolar lavage fluid kuti muunikenso.

Zogwirizana nazo:

SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Njira)