Kupangidwa kwa injini ya nthunzi kunabweretsa kusintha kwa mafakitale, kupita patsogolo kwa makompyuta kunapanga dziko lamakono lamakono, ndipo kusintha kwaumisiri kudzabweretsadi kupita patsogolo kwa nthawi!Kubadwa kwaCell Direct RT-qPCR Kitya Foregene yatsala pang'ono kubweretsa nyengo yatsopano yozindikira mamolekyulu!

7min Pezani RNA template

Inde, mudamva bwino, osakayikira, kuchokera ku cell kupita ku RT-qPCR, tsopano masitepe awiri okha

Lekani Kuchitapo kanthu

Q:Kodi kuchotsa RNA ndikokwanira pa liwiro lothamanga chonchi?Kodi padzakhala kuipitsidwa kwa DNA?

A: Osadandaula, zogulitsa zathu zafananizidwa ndi kutsimikizika kosawerengeka, ndipo palibe kusiyana poyerekeza ndi zoyeserera zachikhalidwe za RT-qPCR.Kuphatikiza apo, kukula kwachitsanzo ndi kocheperako, ndipo kuyesako kumatha kuchitidwa ndi ma cell osachepera 10 otukuka.Kaya ndi mbale ya 6, 12, 24 kapena 96-chitsime, maselo amatha kuponyedwa mwachindunji m'zitsime za mbale, zomwe zimakhala zosavuta komanso zachangu.Ndi 5 × Direct RT Mix ndi 2 × Direct qPCR Mix muzogulitsa, template ya RNA ingagwiritsidwe ntchito kusinthika kwapamwamba komanso kukulitsa kwachindunji, ndi kukhudzidwa kwakukulu, kukhazikika kwamphamvu ndi kukhazikika kwabwino.The reverse transcriptase qPCR kit ndi yosavuta.

Cell Direct RT-qPCR Kitsikuti ili ndi dongosolo lapamwamba la lysis, komanso limakhala ndi mphamvu zotsutsana ndi RT-qPCR reaction inhibitors, zomwe zimapangitsa kuti chida chonsecho chikhale ndi malire otsika kwambiri, ndipo chikhoza kupeza zotsatira zabwino pa maselo a 10. Njira imodzi iyi ya RT qPCR ndi yabwino kwambiri.

Tsopano pali njira zobiriwira za SYBR qPCR ndi Taqman qPCR zomwe zilipo.