Kutulutsa kwa Nucleic acid ndi luso loyambira pakuyesa kwa biology ya maselo.Kaya ikhoza kuchita bwino ndikugwiritsa ntchito lusoli mwaluso chakhalanso chofunikira kwa oyesera pambuyo pake kuti apititse patsogolo kukhala akatswiri oyesera.
Malinga ndi zida zosiyanasiyana zofufuzira, oyesera amagawidwa m'magulu atatu awa:
Animal major, Plant major, Microbiology major
Mtundu uliwonse woyesera upitiliza kukopera makope kuti ukhale ndi luso lapadera la zitsanzo zinazake.
Masiku ano, mkonzi akufuna kubweretsa zida ndi zida zomwe sizipezeka kudzera m'ndende kwa oyesera.Ndi iwo, mutha kukweza mwachangu mbali zonse.Simaloto kukhala mulungu woyesera.
Nkhaniyi ndi ya akatswiri oyesa mbewu (oyesa ntchito zina athanso kuphunzirapo ~).
Ndikukhulupirira kuti m'badwo uliwonse wa oyesera udzakhala udamvapo za njira zamtundu wamtundu wa nucleic acid zomwe zimaperekedwa kuchokera ku mibadwomibadwo kuchokera kwa omwe adatsogolera.Mayina atatu akulu m'makampani opanga mbewu.
Ngakhale akuluakulu ndi abwino kwambiri, amakhalabe ndi zophophonya zosapeŵeka.
Njira ya TRIzol
Nthawi zambiri amagwiritsidwa ntchito pochotsa
RNA
●Poizoni komanso wovuta
●Kukhala ndi kuipitsidwa kwa ma genome
Njira ya CTAB
Nthawi zambiri amagwiritsidwa ntchito pochotsa
genomeDNA
● Kuwononga nthawi
● Chovuta kwambiri kuchotsa CTAB pochapa kuposa mchere, zotsalira zochepa zimakhala ndi mphamvu yaikulu pa ntchito ya enzyme.
Njira ya SDS
Nthawi zambiri amagwiritsidwa ntchito pochotsa
plasmid
●Sioyenera zomera zokhala ndi ma polysaccharide ambiri
● Kuwonongeka kwa mapuloteni
M'dera lino lamakono lomwe lili ndi maulendo obwereza-liwiro, njira zachikhalidwe zogwirira ntchito sizingathenso kukwaniritsa zosowa za oyesera, ndipo kukonzanso zipangizo kuli pafupi.
Foregene Nucleic Acid Extraction Kit yayesedwa nthawi za N ndi oyesa ambiri.Ndiwosavuta kugwiritsa ntchito, sagwiritsa ntchito ma reagents oopsa, komanso samawonjezera ma enzyme.Nucleic acid yomwe imapezeka ndi yoyera kwambiri, yomwe ndi yofunikira pakugwira ntchito tsiku ndi tsiku kwa oyesera kwambiri.
Foregene RNA Isolation zida
Pano, ndiwululanso njira yobisika ya opaleshoni kwa oyesera, yomwe ndi kusankha njira yoyenera yochotsera molingana ndi mtundu wa zomera (njira yodziwira mtundu wa zomera ili kumapeto kwa nkhaniyo!), Chifukwa chakuti zomera zina za polysaccharides ndi polyphenols ndizochuluka, ndipo njira zachikhalidwe ndizoopsa.Osakwanira kulamulira.
Chabwino, zili ngati izi ...
Njira ya TRIzol inagwada mwachindunji pansi pa zitsanzo zokhala ndi ma polysaccharides ndi ma polyphenols monga masamba a nthochi, masamba a ginkgo ndi masamba a thonje.
Pachifukwa ichi, Foregene wapanga zida ziwiri zothandiza kwa oyesera.Zomera za RNA zotulutsa zomwe zili ndi polysaccharide yotsika ndi polyphenol komanso kuchuluka kwa polysaccharide ndi polyphenol zimatha kupha adani m'njira yolunjika, kotero kuti oyeserawo saopanso zomera zosiyanasiyana.Ingosinthani zida mwachindunji!
Kuphatikiza pa kufunikira kokweza njira yochotsera, ntchito zoyeserera zisanachitike ziyenera kukonzedwanso.
Chifukwa cha mapangidwe apadera a zitsanzo za zomera, mizu ndi tsinde zolimba za zomera zina zimakhala zovuta kupukuta ndi kuphwanya mofanana.
Pogaya, pafupifupi 80% ya anthu amagwiritsa ntchito nayitrogeni wamadzimadzi popera, pomwe osakwana 20% amagwiritsa ntchito magetsi akupera ndi kugaya mikanda.
Ngakhale kupera kwa nayitrogeni wamadzimadzi ndikotsika mtengo komanso kosavuta kugwiritsa ntchito, ndizovuta kwa oyesera omwe ali ndi zofunikira zambiri kuti agaye nthawi iliyonse ndi khama!Ngati matope sanatsukidwe, amayambitsa kuipitsidwa pakati pa zitsanzo.
Zotsatira za kugaya magetsi zimasiyanasiyana kuchokera kwa munthu kupita kwa munthu, ndipo machitidwe ogwiritsira ntchito oyeserawo adzakhala ndi zotsatira zowonjezereka pa kukhulupirika kwa nucleic acid, ndipo ntchito yotsika kutentha imafunika ngati kuli kofunikira.
kotero, akupera mkanda oscillation akhoza kuonedwa ngati zipangizo panopa yosavuta ndi ogwira pogaya.
Mitundu yodziwika bwino ya ma oscillation imaphatikizira kugwedezeka koyima, kugwedezeka kwapang'onopang'ono ndi mawonekedwe atatu (oscillation yopingasa).Komabe, chophwanya minofu ya Foregene chimadutsa mumtundu womwe ulipo ndikutengera njira yatsopano ya 4D oscillation kuti akuperayo akhale ofanana.
Kukwaniritsa zofunikira zosiyanasiyana, zosavuta kugwiritsa ntchito
Ukadaulo wapadera wophwanyidwa wa 4D, kuphwanya kwachangu kumawonjezeka ndi 20%
Chitsanzocho chatsekedwa kwathunthu, palibe kuipitsidwa kwa mtanda
Chete, ≤56 decibels
Fananizani zotsatira za kuyesa ndi wave ~
Zitsanzo zoyesera: 50mg masamba a tirigu (zochepa za polysaccharides ndi polyphenols)
Chotsani mtundu wa nucleic acid: RNA
Kuchuluka kwa malo: 5μl
Zomwe zimagwiritsidwa ntchito ndi Foregene Plant Total RNA Extraction Kit (RE-05011)
Poyerekeza deta ndi electrophoresis graph, tingaone kuti ndende ndi chiyero cha RNA yotengedwa pambuyo makina akupera ndi apamwamba kuposa wa madzi asafe akupera.Pa nthawi yomweyi, chifukwa makina akupera amatha kuswa chitsanzocho kwathunthu, RNA yaying'ono ikhoza kuchotsedwa bwino ndipo umphumphu wa RNA ukhoza kusungidwa.
Zitsanzo zoyesera: 50mg masamba a ginkgo (okwera polysaccharide ndi polyphenol)
Chotsani mtundu wa nucleic acid: RNA
Kuchuluka kwa malo: 5μl
Kugwiritsa Ntchito Foregene Polysaccharide Polyphenol Plant Total RNA Extraction Kit (RE-05021)
Onani gulu ili la zitsanzo zomwe zili ndi ma polysaccharides ndi ma polyphenols.Zotsatira zake zimakhala zoonekeratu.Ndi kuyera komweko, kuchuluka kwa RNA yopukutira pamakina kumakhala pafupifupi kuwirikiza kasanu kuposa kugaya kwa nayitrogeni wamadzimadzi, ndipo RNA yaying'ono ikuwonekabe bwino ~
Ngati mukuwona kuti palibe zida zomwe zili pamwambazi zomwe zimathamanga kwambiri, ndiye kuti mkonzi ayenera kubwera ndi zida zapamwamba kwambiri!
Bzalani Direct PCR mndandanda
Mbali yapadera ya zida izi ndi yakuti masamba a zomera kapena mbewu siziyenera kugwedezeka ndi kusweka, ndipo zimaponyedwa mwachindunji mu chubu cha EP cha lysis, ndipo kusakaniza kwa lysis kungagwiritsidwe ntchito ngati template ya PCR reaction.Ndi yosavuta ngati sitepe imodzi mofulumira!Panthawi imodzimodziyo, mndandanda wa PCR wolunjika wa Foregene Plants uli ndi UNG anti-pollution system, kotero kuti oyesera asakhalenso ndi nkhawa za kuipitsidwa kwa aerosol mu labotale ndipo akhoza kutenga kuyesa mosavuta.
Malangizo achiweruzo:
Momwe mungaweruzire ngati ndi chomera cha polysaccharide polyphenol
Tengani za 20mg minofu chipika ndi kuika mu woyera 1.5ml centrifuge chubu, kuwonjezera 200ul 200mM NaOH njira, ikani pa 95 ℃ kwa 10min, onani kusintha mtundu pambuyo lysis.
1. Ngati mtundu wa lysate ndi wobiriwira kapena wonyezimira wachikasu, zikutanthauza kuti zomwe zili mu minofu ya polyphenols ndizochepa, ndipo tikulimbikitsidwa kugwiritsa ntchito mndandanda wa Foregene Plant.
2. Ngati lysate ndi brownish-chikasu kapena bulauni-wofiira, zikutanthauza kuti minofu polyphenol zili mkulu, ndipo tikulimbikitsidwa kugwiritsa ntchito Foregene Polysaccharide Polyphenol Plant Plus Series.
Nthawi yotumiza: Aug-20-2021
