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W:M'bale, kusindikiza kwa reverse kumalizidwa, ndipo potsiriza sitepe yomaliza - fulorosenti kuchuluka kwa PCR!Hei, m'bale, kodi PCR yeniyeni yeniyeni ndi chiyani?
M:PCR mumadziwa nthawi zonse, sichoncho?
W: Mukudziwa, PCR ndiye polymerase chain reaction, ndipo ndiukadaulo womwe umachulukitsa DNA!
M:Ndi pafupifupi… Ndi njira yaukadaulo yokulitsa ndandanda ya chandamale kupitilira nthawi miliyoni imodzi kudzera muzoyambira zenizeni, koma izi zimangowonetsa kukhalapo kapena kusapezeka kwa mndandanda wazomwe mukufuna, ndipo sangathe kudziwa kuchuluka kwa template yoyambira.Fluorescence quantitative PCR ndi njira yaukadaulo yomwe idapangidwa kuti ithetse vutoli.Nthawi yeniyeni PCR ndi kuchuluka kwa PCR (qPCR) nthawi zambiri zimatengera izi.
W: Kusiyana kwa PCR wamba kuli pa fluorescence quantification?
M: Inde, ndiye kuti, mu gawo lokulitsa la PCR iliyonse, mphamvu ya siginecha ya fluorescence imadziwika, ndipo kuchuluka kwa template koyambirira kumatha kuwerengedwa molingana ndi kusintha kwamphamvu kwa siginecha.
W: Zikumveka zovuta!Kodi ndiwerengere ndekha?
M: Izi zimafuna kuti muphunzire pang'onopang'ono, ndi bwino kuti mumvetse mfundoyi.Tsopano mutha kuyang'ana pa mtengo wa CT poyamba, womwe ndi chidziwitso choyesera, ndipo kukula kwake kumagwirizana ndi kukula kwa template yoyamba.Palinso ma graph monga ma curve amplification ndi ma curve osungunuka.Iwo ali ndi mawu omaliza ngati kuyesa kwanu kukuyenda bwino kapena ayi.
W: O, m'bale.Ndiye ndipanga kuyesa kwa qPCR!
M: Samalani mwapadera kuti musawonjezere ma templates kumalo a reagent, apo ayi kusakaniza kwanu kosagwiritsidwa ntchito kudzakhala koipitsidwa mosavuta!
W: Ndamva!

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Real Time PCR Easyᵀᴹ-SYBR Green I Kit

Kuchita bwino kwambiri, kutsimikizika kwakukulu
High tilinazo, zabwino zogwirizana

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Nthawi yotumiza: Jul-22-2022