Mbiri
M'zaka zaposachedwa, ma extracellular vesicles (EVs) akopa chidwi cha anthu ngati chida chothandizira kuchiza;komabe, chithandizo chamankhwala cha EVs pa endometriosis sichinanenedwe.Endometriosis ndi matenda omwe si owopsa omwe amakhudza 10-15% ya amayi omwe ali ndi zaka zobereka, zomwe zimayambitsa zizindikiro zosiyanasiyana, zomwe zimapangitsa kuchepa kwa moyo komanso kulemetsa kwakukulu.
Mau oyamba a Nkhani
Pa Julayi 20, 2021, gulu lofufuza la Pulofesa Wang Guoyun waku Chipatala cha Qilu ku Shandong University lidasindikiza pepala lofufuzira lotchedwa "M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting Development of Endometriosis" pa Frontiers in immunology, yomwe idakambirana za kuchepa kwa macrophages.Kuthekera kwa nanovesicles (NVs) pochiza endometriosis.
Nkhaniyi ntchito mosalekeza extrusion njira kukonzekera M1NVs, ndi ntchito Co-chikhalidwe njira kuphunzira kusintha angiogenesis, kusamuka, kuwukira ndi zizindikiro zina za eutopic endometrial stromal maselo (EM-ESCs) kwa odwala endometriosis.Panthawi imodzimodziyo, chitsanzo cha mbewa cha endometriosis chinakhazikitsidwa, ndipo mbewa zinathandizidwa ndi PBS, MONVs kapena M1NVs, motero, kuti awunike mphamvu ndi chitetezo cha M1NV pochiza endometriosis.
Zotsatira zinasonyeza kuti mu vitro M1NVs akhoza mwachindunji kapena mwachindunji kuletsa kusamuka ndi kuwukira kwa EM-ESCs, ndi kuletsa angiogenesis.Muchitsanzo cha mbewa, ma M1NV amalepheretsa kuchitika kwa endometriosis kudzera mu M2 macrophage reprogramming popanda kuwononga chiwalo.Zikuwonetsa kuti M1NVs imatha kuletsa mwachindunji kuchitika kwa endometriosis, komanso imathanso kuletsedwa ndi repolarizing macrophages amtundu wa M2 kukhala mtundu wa M1.Choncho, kugwiritsa ntchito M1NVs kungakhale njira yatsopano yochizira endometriosis.
Thandizo la Foregene
Mu phunziroli, chifukwa M1NV inakonzedwa ndi kufinya mosalekeza M1 macrophages, nkhaniyi inagwiritsa ntchito qRT-PCR kuti izindikire zomwe zimayambitsa kutupa ndi zizindikiro za M1 macrophage iNOS, TNF-a ndi IL-6 mRNA mu M1NV ndi M1 macrophages.Kusintha kofananirako.Zotsatira zinawonetsa kuti ma M1NVs anali ndi zolembera zowonjezereka za pro-inflammatory factor mRNA ndi M1 macrophage, zomwe zimasonyeza kuti M1NVs ikhoza kusunga bwino machitidwe a maselo a M1.Njira yofufuzirayi imagwiritsa ntchito QuickEasy Cell Direct RT-qPCR Kit-SYBR Green I ya Foregene.
Cell Direct RT-qPCR Kitzambiri

Zochitika zantchito:
 
1. Kuwongolera ma gene ndi kusanthula kwamafotokozedwe, kutsimikizira kuchulukitsitsa kwa jini kapena kusokoneza, kuyang'anira mankhwala osokoneza bongo, ndi zina zambiri;
2. Kuzindikira mawu a jini a maselo ovuta kulimidwa monga maselo oyambirira, maselo amtundu, ndi maselo a mitsempha;
3. Kuzindikira mRNA mu zitsanzo monga exosomes ndi nanovesicles.
Mawonekedwe: