Lofalitsidwa ndi: CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
IFE: 69.504
Zothandizira:Cell Total RNA Isolation Kit
Ulalo Woyambirira:https://www.nature.com/articles/s41586-022-05271-2
Matenda a filovirus monga Ebola virus (EBOV) ndi kachilombo ka Marburg angayambitse zizindikiro zoopsa zachipatala, kuphatikizapo kutentha kwa thupi komanso kulephera kwa ziwalo zambiri.4.Apa tidatsimikiza kapangidwe ka Ebola virus L protein muzovuta ndi tetrameric VP35 pogwiritsa ntchito ma cryo-electron microscopy (boma 1).Kusanthula kwadongosolo kunawonetsa kuti kachilombo ka Ebola L ali ndi filovirus-enieni yoyika chinthu chomwe chili chofunikira pakupanga kwa RNA, komanso kuti VP35 imalumikizana kwambiri ndi N-terminal dera la L ndi ma protomer atatu a VP35 tetramer.
Mu pepala ili, RNA yonse idatulutsidwa pogwiritsa ntchito ForegeneCell Total RNA Isolation Kitndipo kuchuluka kwa RNA kunayesedwa ndi Nanodrop pa 260 nm, ndiyeno kupanga RT/qPCR kuzindikira.
■ Njira yonseyi imayendetsedwa ndi kutentha kwa chipinda (15-25 ℃), popanda kusamba kwa ayezi ndi kutentha kochepa kwa centrifugation.
■ Zida zonse ndi RNase-Free, palibe chifukwa chodera nkhawa za kuwonongeka kwa RNA.
■ DNA-Cleaning Column imamangiriza DNA, kotero kuti zida zitha kuchotsa kuipitsidwa kwa DNA popanda kuwonjezera DNase.
■ Zokolola zambiri za RNA: Mzere wa RNA-okha ndi fomula yapadera imatha kuyeretsa RNA bwino.
■ Kuthamanga kwachangu: kosavuta kugwiritsa ntchito ndipo kumatha kumalizidwa mu mphindi 11.
■ Chitetezo: Palibe organic reagent yomwe imafunikira.
■ Ubwino wapamwamba: RNA yoyeretsedwa ndi yoyera kwambiri, yopanda mapuloteni ndi zonyansa zina, ndipo imatha kukumana ndi mayesero osiyanasiyana.
Nthawi yotumiza: Nov-04-2022
