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Kaya ndinu mwana watsopano ku biological, kapena bwana wamkulu yemwe ali ndi luso lamphamvu ndi mphamvu, kodi munayamba mwavutikirapo kuchotsa ma RNA apamwamba kwambiri?Ndi anthu angati omwe amawononga zitsanzo zamtengo wapatali chifukwa chazovuta komanso zovuta kugwiritsa ntchito njira yachikhalidwe ya Trizol?

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Tsopano, Uthenga Wabwino ukubwera!Tsanzikanani kwathunthu ndi njira zachikhalidwe zowononga nthawi komanso zosagwira ntchito, ndikuchotsa mosavuta RNA yonse yamtengo wapatali m'mphindi 16.

Cell Total RNA Isolation Kit

Dongosolo lilibe RNase, palibe chifukwa chochotsa chloroform, palibe mapuloteni ndi zonyansa zina, kotero mutha kukhala otsimikiza.

Kaya ndinu ongoyamba kumene kapena bwana wamkulu, mudzakhala ndi miyezo yanu yapamwamba pamtundu wa RNA m'zigawo.

Ndiye zida zoyenera ndi zotani?

Mwachidule, zikutanthauza "zambiri ndi bwino."

Palibe vuto, kwaniritsani umbombo wanu.

Chidachi chimatha kutulutsa RNA yonse m'maselo opangidwa mu 96, 24, 12, ndi 6-chitsime mbale zokhala ndi zotulutsa zambiri.Pali mitundu yambiri ya RNA yobwezeretsedwa.Kuphatikiza pa zidutswa zodziwika bwino za 28s ndi 18s, 5s ndi tiziduswa tating'ono ta miRNA zitha kugwiritsidwanso ntchito.Zosonkhanitsidwa.

Gawo lapadera loyeretsa DNA limakupatsani mwayi wochotsa ma genomic DNA osawonjezera DNase, ndipo zotsatira zake zimakhala zabwinoko, kukwaniritsa zomwe mukufuna kuyesa.

Mukuwona apa, kodi onse asunthidwa?Osadandaula, chinthu chabwino, lankhulani ndi zowona!!!

一、Zosavuta!(16min sikulankhula chabe)

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二、pamwamba kwambiri!(Zotsatira zimawonekera)

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Foregene Cell Total RNAIsolation Kits Kuyerekeza zotsatira za njira ya adsorption column ndi njira ya Trizol

三, Wokhazikika!(Mukagwiritsa ntchito mudzati ndizabwino)

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Mtundu wa Foregene QPCR wa jini A pambuyo polemba m'mbuyo

Malangizo:

Pamene kuchuluka kwa maselo kumakhala kochepa kwambiri, zokolola zonse za RNA zidzachepetsedwa, ndipo spectrophotometer sangathe kudziwa molondola mtengo wa OD.Ndikofunikira kuti muyese mayeso amtundu wa qPCR wotsatira.Izi zimagwira ntchito bwino ndi kampani yathu RT EasyTM II (First-strand cDNA synthesis for Real Time PCR) (RT-01022)!

Sankhani Foregene Cultured Cell Total RNA Extraction Kit kuti kuyesa kwanu kukhale gawo limodzi mwachangu.Mukuyembekezera chiyani, chitanipo kanthu tsopano!


Nthawi yotumiza: Jul-08-2021