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lncRNA mawonekedwe:

1. lncRNA nthawi zambiri imakhala yayitali, yokhala ndi mawu osinthika komanso njira zophatikizira zosiyanasiyana panthawi yosiyanitsa;

2. Poyerekeza ndi jini zolembera, lncRNA nthawi zambiri imakhala yotsika;

3. Ma lncRNA ambiri amakhala ndi mawonekedwe owoneka bwino anthawi ndi malo munjira yakusiyanitsa ndi chitukuko;

4. Pali khalidwe mawu zotupa ndi matenda ena;

5. Malo a subcellular a lncRNA ndi osiyanasiyana;

6. Zotsatizanazi ndizochepa posungirako, koma ntchitoyo ili ndi mlingo wina wotetezera ndi zina zotero.

LncRNA reverse transcript solution:

Tikudziwa kuti lncRNA ili ndi mawonekedwe apamwamba m'maselo.RNA yotereyi nthawi zambiri imakhala ndi zida zovuta monga malupu a tsinde kapena ma hairpins.Ngati dongosolo lachiwiri la lncRNA silingathetsedwe bwino, zolembera zobwerera m'mbuyo zimakhala zolephera kapena zosapambana pochita RT-qPCR.Vuto la mphamvu zochepa.

Nthawi zambiri, pali mfundo zitatu zofunika kuti tipeze ma tempulo apamwamba kwambiri a cDNA:

Ubwino wa template ya RNA, zoyambira zolembera zolembera ndi reverse transcriptase, ndi reverse transcriptase ndiye chinthu chofunikira kwambiri.The RNA oyeretsedwa ndi ambiri ntchito RNA m'zigawo reagents nthawi zambiri amakhala zotsalira za mowa ndi guanidine mchere, amene amphamvu chopinga zotsatira zambiri reverse transcriptases;ngati kutentha koyenera kwa reverse transcriptase reaction kuli kochepa, sikungakhale koyenera ku RNA.Kutsegula kwa dongosolo la hierarchical.

Pakali pano, kutentha koyenera kwa reverse transcriptase komwe kumagwiritsidwa ntchito m'ma laboratories ambiri nthawi zambiri ndi 42°C, ndipo kutentha kwakukulu kumatha kufika pafupifupi 50°C.M'malo mwake, kutsegulidwa kwa mawonekedwe apamwamba a RNA kumafuna kutentha kwambiri.

Kutentha kwa kupanga cDNA yoyamba ya Fortune Biotech 2 × RT Easy TM Mix ndi yokwera kwambiri mpaka 50 ℃-65 ℃, zomwe zimathandiza kuti zithetseretu mapangidwe apamwamba a lncRNA, ndipo nthawi yogwiritsira ntchito ndi yochepa.

Kuphatikiza apo, mankhwalawa alinso ndi mphamvu zotsutsana ndi kusokoneza kwa RNA yokhala ndi mowa wotsalira ndi mchere wa guanidine, womwe ungathe kukwaniritsa zofunikira za ma laboratories kuti akwaniritse zolembera zapamwamba kwambiri.

kumasulira1

Choyamba Strand cDNA Synthesis Kit

Katunduyo nambala: RT-01011 (25 nthawi)

Mawonekedwe:

1. Kukhazikika kwamafuta: 65 ℃ ikadali ndi ntchito yabwino yolembera, yomwe imathandiza kutsegula dongosolo lachiwiri lovuta.

2. Kulekerera: Kuphatikizika kwa cDNA kumatha kuchitidwabe bwino pazithunzi za RNA mosayera bwino (zotsalira zamchere za ethanol ndi guanidine)

3. Mwachangu: ntchito yosavuta, kaphatikizidwe koyambirira ka cDNA katha kutha mkati mwa mphindi 25-35

Mapulogalamu otsika: PCR, qPCR, RACE, kulemba chip, kumanga laibulale, etc.

Kuphatikiza apo, FJ Biotech imapereka mayankho athunthu a RT-PCR kuti akwaniritse zosowa zamakasitomala osiyanasiyana, kuphatikiza zida zotsatirazi:

kumasulira1

First Strand cDNA Synthesis Kit (Ya qPCR)

Katunduyo nambala: RT-01021 (nthawi 50)

Mawonekedwe:

1. Kusavuta: Zoyambira zolembera zosinthira zawonjezedwa kuti ntchitoyi ikhale yosavuta

2. Kukhazikika kwamafuta: 65 ℃ ikadali ndi ntchito yabwino yolembera, yomwe imathandiza kutsegula dongosolo lachiwiri lovuta.

3. Kulolerana: kaphatikizidwe ka cDNA katha kuchitidwabe bwino pa ma templates a RNA osayera bwino

4. Sensitivity: oyenera zitsanzo zochepa za RNA

Kugwiritsa ntchito pansi: Zoperekedwa mwapadera kwa qPCR yotsatira

zolemba3

Gawo limodzi la RT-PCR zida

Katunduyo nambala: RT-02011 (100 nthawi)

Mawonekedwe:

1. Kukhazikika kwamafuta: 65 ℃ ikadali ndi ntchito yabwino yolembera, yomwe imathandiza kutsegula dongosolo lachiwiri lovuta.

2. kulolerana: cDNA kaphatikizidwe akadali bwinobwino anachita pa RNA zidindo ndi osauka chiyero

3. Zachidziwitso: PCR MIX yokhala ndi zida imathandizira bwino kukulitsa bwino komanso momwe zimachitikira

Ntchito zotsika: cDNA cloning, kuyesa majini ndi kuyesa kwina kwa ma cell

mawu4

Zida ziwiri za RT-PCR

Katunduyo nambala: RT-02021 (100 nthawi)

Mawonekedwe:

1. Zosavuta komanso zachangu: machitidwe a chubu limodzi

2. Kukhazikika kwamafuta: 65 ℃ ikadali ndi ntchito yabwino yolembera, yomwe imathandiza kutsegula dongosolo lachiwiri lovuta.

3. Zachidziwitso: Foregene Taq yowonjezeredwa imathandizira bwino kukulitsa bwino komanso momwe zimachitikira

Ntchito zotsika: kuyesa kwa mamolekyu monga kuyesa kwa majini

mawu5

Gawo limodzi la RT-qPCR kit-SYBR Green I

Katunduyo nambala: RT-02111 (100 nthawi)

Mawonekedwe:

1. Zosavuta: zomwe chubu limodzi

2. Kukhudzika kwakukulu: kumatha kuzindikira ma templates otsika

3. Kugwirizana: koyenera pazida zambiri za fluorescence

Ntchito zotsika: kuzindikira kwa zitsanzo za RNA monga ma cell ndi ma virus

mawu6

Gawo limodzi la RT-qPCR kit-Taqman

Katunduyo nambala: RT-02131 (100 nthawi)

Mawonekedwe:

1. Mwachangu: zindikirani jini yomwe mukufuna

2. Kukhudzika kwakukulu: kumatha kuzindikira ma templates otsika

3. Kugwirizana: koyenera pazida zambiri za fluorescence

Ntchito zotsika: kuzindikira kwa zitsanzo za RNA monga ma cell ndi ma virus
Forge Bioreverse Transcription Kit ikhoza kukuthandizani kuthetsa vuto lolemba mobwerera.Nthawi yomweyo, imagwirizana ndi Forge Bio RNA Extraction Kit (yomwe imatha kuchotsa zotsalira za DNA) kuti ikupatseni yankho lathunthu loyesera la RNA.


Nthawi yotumiza: Aug-27-2021