Njira za PCR ndi kuipitsidwa kwa nucleic acid aerosol m'ma labotale oyesa ma nucleic acid zili ngati mbali ziwiri zandalama.Tingasankhe kukhala nazo kapena ayi, koma sitingathe kusankha kaya tizifuna kapena kuzigwiritsa ntchito.

1. Kuunika kwa chochotsa DNA

Kuti mukwaniritse kuchotsedwa kwa ma nucleic acid aerosol, ndikofunikira kuyang'ana zochotsa ma DNA zomwe zimatha kuchotsa ma nucleic acid mumadzimadzi.Chifukwa palibe zambiri zochotsa DNA zomwe zimagwira ntchito.Kwa njira yoyesera, chonde onani: Kuchotsa DNA sikungakhale "ngodya yachinsinsi" ya labotale!

Pakuyesa uku, makope a 100 / μL (CT pafupifupi 31) a ASFV plasmid ndi DNA remover yowerengedwa ndi digito PCR adasakanizidwa molingana, kenako adachita kutentha kwa 10min, 20min ndi 30min motsatana.Pambuyo pochotsa nucleic acid, kukulitsa kwa qPCR kunachitika.Kuwongolera kwabwino kosakanikirana ndi plasmid ndi madzi kunafanizidwa.Popeza kuyesako sikunamalizidwe nthawi yomweyo, pangakhale kusiyana kwina pakati pa zotsatira, koma sizimakhudza mapeto a kuyesa.Pakadali pano, ndawunika zinthu 10 zochotsa DNA zamalonda.Nambala 1 yokha, No. 6 ndi No.Mankhwala ena alibe pafupifupi chilichonse.

Table 1 Nucleic acid kuchotsa zotsatira za malonda a DNA remover

2. Kuyesa kwa DNA kuchotsa zotsatira za mankhwala omwe amagwiritsidwa ntchito kwambiri

1. Kuwunika mankhwala omwe amagwiritsidwa ntchito kwambiri

Pali mitundu yambiri ya mankhwala ophera tizilombo omwe amagwiritsidwa ntchito kwambiri m'ma laboratories: aldehydes, phenols, alcohols, quaternary ammonium salt, peroxides, kukonzekera kwa chlorine, ndi zidulo ndi maziko.Zotsatira zakupha tizilombo toyambitsa matendawa pa tizilombo tating'onoting'ono tatsimikiziridwa mokwanira ndi zoyesera, koma Palibe deta yoyesera yokwanira pakuwonongeka kwa ma nucleic acid.Ma labotale oyesa ma Nucleic acid amafunikira mankhwala ophera tizilombo omwe samangokhala ndi zotsatira zabwino zophera tizilombo, komanso amatha kuwononga DNA.Zotsatira zoyesa zikuwonetsa kuti mankhwala awiri okha a chlorine a 84 opha tizilombo ndi trichloroisocyanuric acid ndi 1 M hydrochloric acid amakwaniritsa zofunikira.Popeza 1 M hydrochloric acid imawononga kwambiri, tikulimbikitsidwa kuti mankhwala a chlorine agwiritsidwe ntchito ngati kutaya zinyalala m'ma laboratories oyesa ma nucleic acid.Komabe, kukonzekera kwa chlorine kumawononga zitsulo ndipo sikungagwiritsidwe ntchito popha zida ndi zida.

Table 2 DNA kuchotsa zotsatira za mitundu yosiyanasiyana ya mankhwala ophera tizilombo

2. The osachepera ogwira ndende ya mankhwala klorini

Mankhwala ophera tizilombo toyambitsa matenda a klorini ali ndi mphamvu yowonongeka ya DNA, koma chifukwa cha zitsulo zawo zowonongeka ndi kupsa mtima, amatha kugwiritsidwa ntchito kuyeretsa pansi, zopangira zitsulo zopanda zitsulo, nsonga zonyowa, machubu a centrifuge ndi zinthu zina zoyesera.

Malinga ndi "New Coronary Pneumonia Epidemic Disinfection Technical Guidelines": Zowononga (magazi a odwala, zotuluka ndi masanzi) zilipo klorini 5g/L-10g/L mankhwala ophera tizilombo toyambitsa matenda;Pansi, makoma, ndi zinthu zina amagwiritsa ntchito klorini 1g/L mankhwala ophera tizilombo toyambitsa matenda: zovala, zofunda ndi nsalu zina zimanyowa poyamba mu mankhwala ophera tizilombo toyambitsa matenda a chlorine ndi 0.5g/L wopezeka kwa chlorine kwa mphindi 30, kenako amatsukidwa monga mwa nthawi zonse.

Table 3 DNA kuchotsa zotsatira za woipa woipa wa chlorine mankhwala

Zotsatira zoyezetsa zikuwonetsa kuti: chlorine ikakhala yochuluka kuposa kapena yofanana ndi 1.2 g/L, mankhwala ophera tizilombo toyambitsa matenda okhala ndi klorini amatha kutsitsa plasmid 100/μL akagwiritsidwa ntchito kwa mphindi zisanu.Pochita kwa mphindi 10, mankhwala ophera tizilombo 84 okhala ndi chlorine wochuluka kuposa kapena wofanana ndi 0.6 g/L amatha kunyozetsa makope 100/μL a plasmids.

3. Kuyesera kuchotsa mpweya woipitsidwa ndi nucleic acid aerosol

Momwe mungayeretsere mpweya woipitsidwa ndi nucleic acid aerosols?Malingaliro ambiri ndi kupanga malo okhala ndi aerosol ndikuyeretsa, kapena kuyeretsa ma nucleic acid pambuyo poipitsidwa.Kwa ine, kuyesa komwe sikungabwerezedwe ndipo zotsatira za kuyesako sizingawunikidwe mochulukira zilibe tanthauzo, kotero ndidabwereka njira zina zophera tizilombo toyambitsa matenda mumlengalenga.Onani "GB27948-2020 General Zofunikira pa Opha tizilombo toyambitsa matenda a Air" ndi "Mafotokozedwe aukadaulo a Disinfection"

1. Zida zoyesera

1.1 DNA remover: Pakuyesa uku, zotulutsa ziwiri za DNA zomwe zimapezeka pamalonda 6 ndi 8 mu Table 1 zidasankhidwa.

1.2 Mayeso a plasmid: Poganizira kuti mtengo wa CT wa kuwonongeka kwa labotale udzakhala wapamwamba kuposa 30, kuchuluka kwa ASFV gene plasmid yomwe imagwiritsidwa ntchito nthawi ino ndi pafupifupi 100 makope / μL, ndipo fulorosenti yochulukira PCR CT ndi pafupifupi 31.07.Onjezani plasmid ku swab ya thonje yoyandama ndikuwuma mpweya.

1.3 Test chida: Bilingkehan KVBOX disinfection chida, amene mokoma mtima mothandizidwa ndi Shenzhen Runlian Environmental Technology Co., Ltd., ali kwambiri mpweya disinfection ntchito ndi hydrogen peroxide tizilombo toyambitsa matenda, ndipo angathe kupha spores ndi tizilombo tina.

1.4 Malo oyesera: zenera lotsekeka losamutsa la pafupifupi ma kiyubiki mita 0.1.

2. Njira yoyesera

Kuchuluka kwa mankhwala ophera tizilombo pamakina ophera tizilombo ndi 10ml, ndipo kuchuluka kwake ndi 100ml/m3, komwe ndikwambiri kuposa "GB27948-2020 General Requirements for Air Disinfectants": mukamagwiritsa ntchito kupopera kwa aerosol, kuchuluka kwa mankhwala ophera tizilombo kuyenera kukhala ≤10 ml/m3.