MOONI, abwenzi!
Pambuyo kuchita nucleic asidi m'zigawo kwa zaka zambiri, aliyense amadziwa bwino mfundo yakuti khalidwe nucleic asidi m'zigawo ndi chinsinsi cha kupambana ndi kulondola kwa zoyesa wotsatira.
Nthawi zambiri, timangosamala ngati chiŵerengero cha OD260/OD280 chili mkati mwamulingo woyenera.Monga chonchi…
Mwambiwu umati, sungathe kulankhula kwambiri, ndipo sungakhale wosangalala msanga kwambiri.
Choyamba mverani Mkonzi akuuzeni kankhani kakang'ono!
Nthano zokongola zotere nthawi zambiri zimachitika m'ma laboratories omwe amachotsa nucleic acid.
Mumaganiza kuti mwangotenga RNA, Pepani!
M'malo mwake, kungoti simukudziwa kuti Mr Protein akubisala mobisa.
Kodi mukuganiza kuti mukufuna kupanga Mr Protein kuwoneka ndi OD260/OD280 imodzi yokha?
Wachichepere kwambiri!Zosavuta!
Tiyeni tigwiritse ntchito njira yovuta kwambiri yodetsa siliva kuti ikuthandizireni kuwona mapuloteni obisika mu RNA!
| Zogwiritsidwa ntchito | OD260/280 | Mchida chochepetsera |
| Foregene | 2.09 | Thermo SayansiTMNanoDropTMMmodzi Ultra-trace UV-Vis Spectrophotometer |
| Trizol | 1.91 |
Kalekale, mapuloteni ndi RNA anali mabwenzi apamtima.
Mr Protein ndi amanyazi kwambiri.
Chifukwa chake nthawi zonse yakhala RNA yogwira ntchito komanso yansangala kusewera nayo.
Tsiku lina.
RNA idauza Mr Protein kuti ilandilidwa ndi ena, ndipo a Protein anali achisoni kwambiri.
RNA imakananso kwa Mr protein, nati: "Bwanji ndinakuzembera, umabisala m'zovala zanga, kuyesera kuti usawonekere kwa ena, chabwino?"
Bambo Protein anayamba kumwetulira ndipo anavomera mosangalala.
Patsiku la kulera, Mr Protein adabisala mu zovala za RNA ndipo sanapezeke.Anatsatira munthu woleredwayo kubwerera kwawo ndipo anakhala ndi moyo wosangalala kuyambira pamenepo.
Kuyerekeza kwa kuchotsa kwa RNA ndi ForegeneSeloZonseRNA Extraction Kit
ndi T Company's Trizol Reagent
Titha kuwona bwino njira ya 4 (Trizol), pomwe msewu wa 3 (Foregene) ndiwoyera.Zotsatirazo zinatsimikizira kuti zonyansa za mapuloteni a RNA otengedwa ndi njira ya Trizol akadali madontho ambiri!Ndipo lane 3 (Forgene) ilibe zonyansa zomanga thupi chifukwa woyesayo adagwiritsa ntchito zida za Foregene RNA!(Kutsatsa kudabwera mosayembekezeka)
Zambiri:
https://www.foreivd.com/cell-total-rna-isolation-kit-product/
Nthawi yotumiza: Feb-10-2022
