Nkhani Zamakampani
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Samalani izi kuti muwonjezere kuchuluka kwa zoyeserera zanu za RT-qPCR!
RT-qPCR ndiye kuyesa koyambirira kwa biology ya maselo, ndipo aliyense ayenera kuidziwa bwino.Zimaphatikizanso masitepe atatu: kutulutsa kwa RNA, kusindikizanso ku cDNA, ndi PCR yeniyeni ya fulorosenti.Sizikuthandizira, chikuchitika ndi chiyani?Zikutheka kuti pali vuto ndi...Werengani zambiri -
Kutulutsa kwa RNA, 260/230 ndiyotsika kwambiri, ndipo mtengo wa pcr run ndiwokwera kwambiri.Kodi kuthetsa izo?
Chiŵerengero chochepa cha A260/A230 nthawi zambiri chimayamba chifukwa cha zonyansa zomwe zimakhala ndi kutalika kwa kutalika kwa 230nm.Tiyeni tiwone zomwe zonyansazi zikuphatikiza: Zowononga wamba Mayamwidwe wavelength Ratio zotsatira Protein ~ 230nm ndi 280nm Kuchepetsa munthawi yomweyo kwa A 260 /A 280 ndi A 260 / A ...Werengani zambiri -
Fluorescent quantitative PCR (qPCR) - kapangidwe koyambirira
Pakuyesa kwa qPCR, mapangidwe oyambira nawonso ndi ulalo wofunikira kwambiri.Kaya zoyambira zili zoyenera kapena ayi zimagwirizana kwambiri ndi ngati kukulitsa bwino kumafika pamlingo, kaya zokulitsa zili zenizeni, komanso ngati zotsatira zoyeserera zilipo.Ndiye kupanga bwanji ...Werengani zambiri -
Zowonjezera zomwe zimachitika pa PCR ndi ntchito zake
Ndikukhulupirira kuti aliyense nthawi zonse amakumana ndi zovuta zoterezi kapena zotere pochita machitidwe a PCR, koma ambiri a iwo akhoza kugawidwa m'magulu awiri: Kukulitsa pang'ono kwa template ya jini (kukulitsa);Kuchulukitsitsa kwa jini kopanda cholinga.Kugwiritsa ntchito zowonjezera ndi chimodzi mwazinthu zodziwika bwino ...Werengani zambiri -
Chidule cha mankhwala 45 a gene therapy omwe agulitsidwa padziko lonse lapansi
Majini ndi mayunitsi oyambira omwe amawongolera mawonekedwe.Kupatulapo majini a mavairasi ena, amene amapangidwa ndi RNA, majini a zamoyo zambiri amapangidwa ndi DNA .Matenda ambiri a zamoyo amayamba chifukwa cha kugwirizana kwa majini ndi chilengedwe.Gene therapy imatha kuchiritsa matenda ...Werengani zambiri -
Munthawi ya chithandizo cha RNA, ndani angakhale "wokondedwa watsopano" wamakampani |Zowerengera zapachaka
Gwero: WuXi AppTec Zaka zaposachedwa, gawo la chithandizo cha RNA lawonetsa kuphulika- m'zaka 5 zapitazi zokha, njira zochiritsira 11 za RNA zavomerezedwa ndi FDA, ndipo chiwerengerochi chimaposanso kuchuluka kwamankhwala ovomerezeka a RNA!Poyerekeza ndi mankhwala azikhalidwe, RNA therapy ca...Werengani zambiri -
PCR wamba, fulorosenti PCR, digito PCR;kusanthula ubwino ndi kuipa!
Zosintha zingapo m'mbiri yaukadaulo wozindikira m'malingaliro mwanga ndiukadaulo wa immunolabeling potengera mfundo yomangiriza ma antigen-antibody, ukadaulo wa PCR ndi ukadaulo wotsatizana.Lero tikambirana zaukadaulo wa PCR.Malinga ndi t...Werengani zambiri -
Chidule|Pofika mu Ogasiti 2022, mankhwala 41 a gene akhazikitsidwa padziko lonse lapansi.
Posachedwapa, mankhwala atatu ochizira jini avomerezedwa kuti azigulitsidwa, zomwe ndi: (1) Pa Julayi 21, 2022, PTC Therapeutics, Inc. (NASDAQ: PTCT) idalengeza kuti AAV gene therapy Upstaza™ idavomerezedwa ndi European Commission Ndiwoyamba kugulitsidwa gene therapy mwachindunji ...Werengani zambiri -
Zakale ndi Zamakono Za CACLP
Kuyambira pa Okutobala 26 mpaka 28, 2022, phwando latsopano lamankhwala oyesera komanso matenda a in vitro - The 19th China China Association of Clinical Laboratory Practice Expo The 2nd China IVD Supply Chain Expo (CISCE) idachitikira bwino ku Nanchang Greenland International Expo Center.FOREGENE Co...Werengani zambiri -
PCR makina|Kodi mukumvetsa?
PCR makina|Kodi mukumvetsa?Ukadaulo wa PCR wopambana Mphotho ya Nobel Mu 1993, wasayansi waku America Mulis adalandira Mphotho ya Nobel mu Chemistry, ndipo zomwe adachita zinali kutulukira luso la PCR.Matsenga aukadaulo wa PCR ali m'makhalidwe awa: Choyamba, kuchuluka kwa DNA ku ...Werengani zambiri -
Thesis Yaposachedwa Yachilengedwe: Unikani kapangidwe ndi kudula kachitidwe ka DNA ka chida chatsopano chosinthira jini ISRB
M'zaka khumi zapitazi, teknoloji yokonza jini yochokera ku CRISPR yakula mofulumira, ndipo yagwiritsidwa ntchito bwino pochiza matenda a chibadwa ndi khansa m'mayesero a anthu.Nthawi yomweyo, asayansi padziko lonse lapansi amangogwiritsa ntchito zida zatsopano pogwiritsa ntchito gene editing po...Werengani zambiri -
Gene mankhwala, kwathunthu "kudzutsa" khutu
Kutaya kumva (HL) ndi matenda omwe amapezeka kwambiri mwa anthu.M'mayiko otukuka, pafupifupi 80% ya milandu prelingual ugonthi ana amayamba chifukwa cha majini.Zofala kwambiri ndi zolakwika zamtundu umodzi (monga momwe ziwonetsedwera mkuyu.Werengani zambiri
