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PCR Hero ndi
◮Kukhulupirika kwakukulu: 6nthawi ya enzyme wamba ya Taq;
◮Mofulumira aliwiro lokulitsa:liwiro lokulitsa ndi 5-10sec/kb, lomwe ndi 6-12 nthawi ya enzyme wamba ya Taq;
◮Zambiri zosinthika za template: imatha kukulitsa bwino ma tempulo osiyanasiyana ovuta a DNA okhala ndi ma GC apamwamba komanso ovuta kukulitsa;
◮ZapamwambaKukulitsa luso: kuchuluka kwa machulukidwe am'mimba ndi otsika kuposa ma enzyme wamba a Taq;
◮Smotalika Kulekerera kwachilengedwe: imayikidwa pa 37 ° C kwa sabata, kusunga kuposa90%ntchito;
◮Ili ndi 5'→ 3' DNA polymerase ntchito ndi 5'→ 3' exonuclease ntchito, popanda 3'→ 5' ntchito exonuclease.
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Pamwamba