PCR Heroᵀᴹ (Yokhala Ndi Dye)
Kufotokozera Kwa Zida:
◮Kukhulupirika kwakukulu: nthawi 6 kuposa enzyme wamba ya Taq;
◮Liwiro lokulitsa mwachangu
◮Zambiri zosinthika za template
◮Kuchita bwino kwambiri kwa amplification
◮Kulekerera kwachilengedwe kumakhala kolimba: kuyikidwa pa 37 ° C kwa sabata, kusunga ntchito zoposa 90%;
◮Ili ndi 5'→ 3' DNA polymerase ntchito ndi 5'→ 3' exonuclease ntchito, popanda 3'→ 5' ntchito exonuclease.
Zofotokozera
5ml, 10ml, 40ml
PCR Hero (Wokhala Ndi Dye) Extraction kit ikuphatikiza m'badwo watsopano wapadera wa Foregene wa Taq-Foregene Taq Hero, ma dNTP okhathamiritsa, MgCl2, buffer reaction, PCR reaction enhancer, optimizer ndi stabilizer.Dongosolo la 2 × PCR Hero TM Mix (dye) lili ndi kulekerera kwakukulu kwa PCR inhibitors kuposa dongosolo wamba la PCR Mix, ndipo limatha kuthana ndi kukulitsa kwa PCR kwa ma template osiyanasiyana ovuta.Dongosolo lapadera la machitidwe a Taq Hero komanso kuchita bwino kwambiri kwa Taq Hero kumapangitsa kuti machitidwe a PCR akhale ndi magwiridwe antchito apamwamba, kutsimikizika komanso chidwi.
Zida zamagulu
| 0.1U Taq Hero/μl |
| 400μM dNTPs |
| 20mM Tris-HCl(pH8.8) |
| 100mM KCl |
| 5mM MgCl2 |
| Ena stabilizers ndi enhancers |
| DNA Loading Buffer |
Mbali & ubwino
■Kukhulupirika kwakukulu: nthawi 6 kuposa enzyme wamba ya Taq;
■ Kuthamanga kwachangu kokulitsa : Kuthamanga kwa matalikidwe ndi 5-10sec/kb, yomwe ndi 6-12 nthawi ya Taq enzyme wamba;
■ Kusinthasintha kwa ma template: Imatha kukulitsa bwino ma templates osiyanasiyana ovuta a DNA ndi apamwambaZithunzi za GC;
■ Kuchulukitsitsa kwapamwamba: kuchepekera kwa kakulidwe kocheperako kuposa ma enzyme wamba a Taq;
■ Kulekerera kwachilengedwe ndikolimba: kuyikidwa pa 37°C kwa sabata, kusunga ntchito zoposa 90%;
■ Ili ndi 5'→3'DNA polymerase ntchito ndi 5'→3'exonuclease ntchito, popanda 3'→5'ntchito ya exonuclease.
■ Chidacho chimasakanikirana ndi Electrophoresis Loading Buffer, PCR itatha, chitsanzocho chikhoza kuikidwa mwachindunji kwa electrophoresis;ntchito ndi yosavuta komanso yosavuta.
Kit application
Kukulitsa kwa PCR kwa zidutswa za DNA, zilembo za DNA, kutsatizana kwa DNA, PCR kuphatikiza A mchira
Kusungirako ndi moyo wa alumali
Ikhoza kusungidwa kwa nthawi yaitali pa -20°C;kuti mugwiritse ntchito pafupipafupi, imatha kusungidwa ku 4°C kwa nthawi yayitali.
