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Real Time PCR Easyᵀᴹ-SYBR Green I kit
Zosavuta—2X PCR Mix kuti muchepetse zolakwika zoyeserera ndi nthawi yogwira ntchito
Specific-optimized buffer ndi hot-start Taq enzyme imatha kuteteza kukulitsa kosakhazikika komanso mapangidwe a dimer.
Kumverera kwakukulu - kumatha kuzindikira makope otsika a template
Kusinthasintha kwabwino - kumagwirizana ndi zida zenizeni zenizeni za PCR
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RT-PCR Easyᵀᴹ I (Chigawo Chimodzi)
◮Chida cha sitepe imodzi chimathandizira kuti zolembera zosinthika ndi PCR zizichitika mu chubu chomwecho.Zimangofunika kuwonjezera template ya RNA, zoyambira zenizeni za PCR ndi RNase-Free ddH2O.
◮Kusanthula kwanthawi yeniyeni kwa RNA kumatha kuchitika mwachangu komanso molondola.
◮Zidazi zimagwiritsa ntchito makina apadera a Foregene reverse transcript reagent ndi Foregene HotStar Taq DNA Polymerase ophatikizidwa ndi njira yapadera yamachitidwe kuti apititse patsogolo kukulitsa bwino komanso momwe zimachitikira.
◮Dongosolo lokonzekera bwino lomwe limapangitsa kuti zomwe zimachitikazo zikhale ndi chidwi chodziwikiratu, kukhazikika kwamafuta, komanso kulolerana bwino.
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RT-PCR Easyᵀᴹ II(Magawo Awiri)
◮Kusanthula kwanthawi yeniyeni kwa RNA kumatha kuchitika mwachangu komanso molondola.
◮Zidazi zimagwiritsa ntchito makina apadera a Foregene reverse transcript reagent ndi Foregene HotStar Taq DNA Polymerase ophatikizidwa ndi njira yapadera yamachitidwe kuti apititse patsogolo kukulitsa bwino komanso momwe zimachitikira.
◮ Dongosolo lokonzekera bwino lomwe limapangitsa kuti zomwe zimachitikazo zikhale ndi chidwi chodziwikiratu, kukhazikika kwamafuta, komanso kulolerana bwino.
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Real Time PCR Easyᵀᴹ-Taqman
◮Zosavuta—2 × PCR Mix kuti muchepetse zolakwika zoyeserera ndi nthawi yogwira ntchito
◮Specific-optimized buffer ndi hot-start Taq enzyme imatha kuteteza kukulitsa kosakhazikika komanso mapangidwe a dimer.
◮Kumverera kwakukulu - kumatha kuzindikira makope otsika a template
◮Kusinthasintha kwabwino - kumagwirizana ndi zida zenizeni zenizeni za PCR
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PCR Heroᵀᴹ (Yokhala Ndi Dye)
◮Kukhulupirika kwakukulu: nthawi 6 kuposa enzyme wamba ya Taq;
◮Liwiro lokulitsa mwachangu
◮Zambiri zosinthika za template
◮Kuchita bwino kwambiri kwa amplification
◮Kulekerera kwachilengedwe kumakhala kolimba: kuyikidwa pa 37 ° C kwa sabata, kusunga ntchito zoposa 90%;
◮Ili ndi 5'→ 3' DNA polymerase ntchito ndi 5'→ 3' exonuclease ntchito, popanda 3'→ 5' ntchito exonuclease.
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700bp DNA Ladder
◮700bp DNA Ladder ndi chinthu chokonzeka kugwiritsidwa ntchito ndipo chili kale ndi 1x Loading Buffer.Mutha kutenga mwachindunji kuchuluka koyenera kwa Marker kwa electrophoresis malinga ndi zosowa zoyeserera.
700bpDNA Marker Ⅰ imakhala ndi magulu 7 a DNA, magulu a DNA ndi: 100bp (50ng/5μl), 200bp (30ng/5μl), 300bp (30ng/5μl), 400bp (40ng/5μl), 500bp (500bp/50) , 500bp (20b/50) bp (35ng/5μl).
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2000bp DNA Ladder
◮ 2000bp DNA Ladder ndi chinthu chokonzeka kugwiritsidwa ntchito ndipo chili kale ndi 1x Loading Buffer.Mutha kutenga mwachindunji kuchuluka koyenera kwa Marker kwa electrophoresis malinga ndi zosowa zoyeserera.BM2000 DNNARD imakhala ndi 6 DNA, mabanki a DNA ndi awa: 100ng / 5μb (70bp (55ng / 5μb (2000b).
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5000bp DNA Ladder
◮ 2000bp DNA Ladderndi chinthu chokonzeka kugwiritsidwa ntchito ndipo chili kale ndi 1x Loading Buffer.Mutha kutenga mwachindunji kuchuluka koyenera kwa Marker kwa electrophoresis malinga ndi zosowa zoyeserera.BM5000 DNA Marker imakhala ndi magulu 8 a DNA, magulu a DNA ndi: 100bp (50ng/5μl), 250bp (50ng/5μl), 500bp (50ng/5μl), 750bp(75ng/5μl), 1000)ng/0b/5μl), 1000)ng/0b/5μl, 1000)ng/5μl ), 3000bp(30ng/5μl), 5000bp(50ng/5μl).
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PCR Easyᵀᴹ (yokhala ndi utoto)
2 × PCR HeroTM Mix system ili ndi kulolerana kwakukulu kwa PCR inhibitors kuposa njira wamba ya PCR Mix, ndipo imatha kuthana ndi kukulitsa kwa PCR kwama tempulo osiyanasiyana ovuta.Dongosolo lapadera la machitidwe a Taq Hero komanso kuchita bwino kwambiri kwa Taq Hero kumapangitsa kuti machitidwe a PCR akhale ndi magwiridwe antchito apamwamba, kutsimikizika komanso chidwi.
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PCR Easy
Dongosolo lapadera la machitidwe komanso magwiridwe antchito apamwamba a Taq DNA Polymerase amapangitsa kuti ma PCR azichita bwino kwambiri, amvekere komanso amvekere.
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PCR Hero ndi
◮Kukhulupirika kwakukulu: 6nthawi ya enzyme wamba ya Taq;
◮Mofulumira aliwiro lokulitsa:liwiro lokulitsa ndi 5-10sec/kb, lomwe ndi 6-12 nthawi ya enzyme wamba ya Taq;
◮Zambiri zosinthika za template: imatha kukulitsa bwino ma tempulo osiyanasiyana ovuta a DNA okhala ndi ma GC apamwamba komanso ovuta kukulitsa;
◮ZapamwambaKukulitsa luso: kuchuluka kwa machulukidwe am'mimba ndi otsika kuposa ma enzyme wamba a Taq;
◮Smotalika Kulekerera kwachilengedwe: imayikidwa pa 37 ° C kwa sabata, kusunga kuposa90%ntchito;
◮Ili ndi 5'→ 3' DNA polymerase ntchito ndi 5'→ 3' exonuclease ntchito, popanda 3'→ 5' ntchito exonuclease.
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QuickEasyᵀᴹ Real Time PCR Kit-Taqman
◮Makina apadera okhathamiritsa a PCR amapanga 2×QuickEasyTMReal PCR Mix-Taqman imagwirizana kwambiri.
◮Hot-start Foregene HS Taq Polymerase ili ndi mphamvu zokulirapo, kukhudzika kwapamwamba, komanso kukwezedwa kwambiri.
◮2 × QuickEasyTMReal PCR Mix -Taqman amagwiritsa ntchito kachitidwe kapadera kokometsedwa ndi Foregene kuti apititse patsogolo kukhudzika ndi kutsimikizika kwa kafukufuku wotsatizana, womwe ungagwiritsidwe ntchito pozindikira ma genotyping ndikutsitsa manambala ndikupeza zotsatira zolondola.
◮Chogulitsachi chimabwera ndi utoto wamkati wa ROX, womwe ungagwiritsidwe ntchito kuchotsa maziko azizindikiro ndi zolakwika zazizindikiro pakati pa zitsime, zomwe ndizosavuta kuti makasitomala azigwiritsa ntchito mumitundu yosiyanasiyana ya zida za PCR.
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