Chomera tsamba la Direct PCR kit (popanda Zida Zoyeserera) Protocol Direct PCR kuchokera ku Zomera.
Kufotokozera
Izi zimagwiritsa ntchito njira yapadera ya lysis buffer kuti iwononge masamba a zomera.Lysate itha kugwiritsidwa ntchito ngati template popanda kuyeretsedwa.Mukawonjezera zoyambira, PCR Mix ya zida izi zitha kugwiritsidwa ntchito pakukulitsa.
Zofotokozera
0×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Zida zamagulu
Gawo I | Mtengo P1 |
Mtengo P2 | |
6×DNA Loading Buffer | |
Gawo II | 2× Leaf PCR EasyTMSakanizani |
Mbali & ubwino
■ Palibe kuyeretsa kwa DNA kowononga nthawi komanso kokwera mtengo
■ Zochepa
■ Chosavuta-chitsanzo chikhoza kuchitidwa ndi PCR kapena lysis reaction popanda kudula kapena kugaya
■ 2 × Mix, kuchepetsa chitsanzo Mumakonda zolakwa ndi anachita dongosolo kukonzekera nthawi
■ Kukonzekera mwachangu template kumatha kumaliza mphindi 10, kuchita kwa PCR kumatha kutha mphindi 50.
■ High throughput-lysis reaction akhoza kumalizidwa mu 96-chitsime PCR mbale
Zosintha za Kit
Kugwiritsa ntchito: chizindikiritso chosinthidwa ma genetic, genotyping, etc.
Chitsanzo: tsamba la mbewu
Mlingo: Diameter 2-3mm (njira yolunjika), m'mimba mwake 5-7mm (njira ya lysis)
Mtundu wozindikiridwa: chidutswa chandamale ≤1kb