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Chomera tsamba la Direct PCR kit (popanda Zida Zoyeserera) Protocol Direct PCR kuchokera ku Zomera.

Kufotokozera Kwa Zida:

Mwachindunji gwiritsani ntchito tsamba kapena tsamba la lysate ngati template yakukulitsa kwa PCR, popanda kutulutsa kosiyana kwa DNA, kufulumira komanso kukhudzika kwakukulu.

Palibe kuyeretsa kwa DNA kowononga nthawi komanso kokwera mtengo (Nthawi, ntchito ndi mtengo wasungidwa!)

Zinthu zochepa

Zosavuta-chitsanzocho chikhoza kuchitidwa ndi PCR kapena lysis reaction popanda kudula kapena kugaya

2 X Mix-kuchepetsa zolakwika pakukweza zitsanzo ndi nthawi yokonzekera dongosolo

Kukonzekera mwachangu-template kumatha kumaliza mphindi 10, machitidwe a PCR amatha kumaliza mphindi 50

High throughput-lysis reaction itha kumalizidwa mu mbale ya 96-PCR

mphamvu zam'tsogolo


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Kufotokozera

Izi zimagwiritsa ntchito njira yapadera ya lysis buffer kuti iwononge masamba a zomera.Lysate itha kugwiritsidwa ntchito ngati template popanda kuyeretsedwa.Mukawonjezera zoyambira, PCR Mix ya zida izi zitha kugwiritsidwa ntchito pakukulitsa.

Zofotokozera

0×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Zida zamagulu

Gawo I

Mtengo P1

Mtengo P2

6×DNA Loading Buffer

Gawo II

2× Leaf PCR EasyTMSakanizani

Mbali & ubwino

■ Palibe kuyeretsa kwa DNA kowononga nthawi komanso kokwera mtengo
■ Zochepa
■ Chosavuta-chitsanzo chikhoza kuchitidwa ndi PCR kapena lysis reaction popanda kudula kapena kugaya
■ 2 × Mix, kuchepetsa chitsanzo Mumakonda zolakwa ndi anachita dongosolo kukonzekera nthawi
■ Kukonzekera mwachangu template kumatha kumaliza mphindi 10, kuchita kwa PCR kumatha kutha mphindi 50.
■ High throughput-lysis reaction akhoza kumalizidwa mu 96-chitsime PCR mbale

Zosintha za Kit

Kugwiritsa ntchito: chizindikiritso chosinthidwa ma genetic, genotyping, etc.
Chitsanzo: tsamba la mbewu
Mlingo: Diameter 2-3mm (njira yolunjika), m'mimba mwake 5-7mm (njira ya lysis)
Mtundu wozindikiridwa: chidutswa chandamale ≤1kb

Kuyenda kwa ntchito

Chomera Leaf Direct PCR Kit04

Electropherogram

Chomera Leaf Direct PCR Kit05

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