China Plant leaf Direct PCR Plus kit (popanda Zitsanzo Zida) Protocol Direct PCR kuchokera kwa Plant Material Manufacturer and Supplier |Foregene

Chomera tsamba Direct PCR Plus zida (popanda Sampling Tools) Protocol Direct PCR from Plant Material

Chomera tsamba Direct PCR Plus zida (popanda Sampling Zida) Protocol Direct PCR kuchokera ku Plant Material Image Featured

Kufotokozera Kwa Zida:

Mwachindunji ntchito polysaccharide ndi polyphenol tsamba lysate monga Chinsinsi PCR matalikidwe, popanda osiyana m'zigawo za DNA, kudya ndi mkulu tilinazo.

◮Palibe kuyeretsa kwa DNA kowononga nthawi komanso kokwera mtengo (Nthawi, ntchito ndi mtengo wasungidwa!)

◮Zinthu zochepa

◮Zosavuta-chitsanzocho chikhoza kuchitidwa ndi PCR kapena lysis reaction popanda kudula kapena kugaya

◮2 X Mix-kuchepetsa zolakwika pakukweza zitsanzo ndi nthawi yokonzekera dongosolo

◮Kukonzekera mwachangu-template kumatha kumaliza mphindi 10, machitidwe a PCR amatha kumaliza mphindi 50

◮High throughput-lysis reaction itha kumalizidwa mu mbale ya 96-PCR

Titumizireni imelo Tsitsani ngati PDF

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Kufotokozera

Izi zimagwiritsa ntchito njira yapadera ya lysis buffer kuti iwononge masamba a zomera.Lysate itha kugwiritsidwa ntchito ngati template popanda kuyeretsedwa.Mukawonjezera zoyambira, PCR Mix ya zida izi zitha kugwiritsidwa ntchito pakukulitsa.

Zofotokozera

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Zida zamagulu

Gawo I	Mtengo wa PS1
	Mtengo P2
	6×DNA Loading Buffer
Gawo II	Mtengo wa PS2
Gawo II	2× Leaf PCR Easy^TMSakanizani

Mbali & ubwino

■ Palibe kuyeretsa kwa DNA kowononga nthawi komanso kokwera mtengo
■ Zochepa
■ Chosavuta-chitsanzo chikhoza kuchitidwa ndi PCR kapena lysis reaction popanda kudula kapena kugaya
■ 2 × Mix, kuchepetsa chitsanzo Mumakonda zolakwa ndi anachita dongosolo kukonzekera nthawi
■ Kukonzekera mwachangu template kumatha kumaliza mphindi 10, kuchita kwa PCR kumatha kutha mphindi 50.
■ High throughput-lysis reaction akhoza kumalizidwa mu 96-chitsime PCR mbale