Bzalani Mbeu (yachikulu ndi yapakatikati) Direct PCR Plus Kit II-UNG(popanda Zida Zoyeserera)
Kufotokozera
Mankhwalawa amagwiritsa ntchito njira yapadera ya lysis reaction, yomwe imatha kumasula mwamsanga DNA ya genomic kuchokera ku zitsanzo za mbeu zokhala ndi ma polysaccharides apamwamba ndi ma polyphenols (monga nthochi, thonje, ndi zina zotero) kuti zigwiritsidwe ntchito pazochitika za PCR, choncho ndizoyenera kwambiri kuyesa chibadwa chachikulu.Pofuna kukwaniritsa zofunikira za mayeso osiyanasiyana ozindikira, zida izi zitha kugwiritsa ntchito mitundu ingapo ya zitsanzo (monga njere zonse, zodula ting'onoting'ono, kapena zitsanzo zanthaka zingapo) poyesera.Pakati pawo, kwa zitsanzo za mbewu zomwe zimayenera kumera, mutha kusankha kudula zidutswa zazing'onoting'ono (1-5mg) kupatula miluza ya mbewu yoyesera;pazoyeserera zomwe zimafunikira kuyesa ndikuyesa zitsanzo zambiri, mutha kusankha kusakaniza zitsanzo zingapo ndikuziphatikiza.Pambuyo pogaya mu tinthu tating'onoting'ono ta 0.5mm, kuyesako kutha kuchitika (osachepera 0.1% ya chitsanzo chandamale amatha kuzindikirika).
Zofotokozera
50T,200T,500T,2000T
Zida zamagulu
Plant Seed Direct PCR Plus Kit II-UNG Polysaccharide polyphenol mbewu mbewu (yaikulu ndi yapakatikati) mwachindunji PCR kit-UNG | |||||
Kupanga kwa zida | Mtengo wa TP-0318T | Mtengo wa TP-03181 | Mtengo wa TP-03182 | Mtengo wa TP-03183 | |
50 T | 200 T | 500 T | 2000 T | ||
Gawo I | Mtengo wa SSP1 | 24ml ku | 96ml pa | 120ml × 2 | 480ml × 2 |
Mtengo SP2 | 3ml ku | 10 ml pa | 25ml ku | 100 ml | |
6 × DNA Loading Buffer | 1.5ml pa | 1.5ml pa | 1.5ml pa | 1.5ml × 4 | |
Gawo II | Mtengo wa SSP2 | 8ml ku | 32ml ku | 80ml ku | 80ml 4 |
2× Mbewu PCR EasyTM Mix | 500μl | 1mlx2 pa | 1.7ml × 3 | 1.7ml × 12 | |
Pamanja | 1 | 1 | 1 | 1 |
Mbali ndi ubwino
- Palibe kuyeretsa nthawi komanso mtengo wa DNA.
- Zofuna zachitsanzo ndizochepa, ingotengani mbeu imodzi.
- Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo ntchitoyo ndi yosavuta.
- Makina okhathamiritsa a PCR amathandizira PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.
- Anti-kuipitsa PCR dongosolo 2 × Mbewu PCR EasyTM Mix (UNG), yomwe imatha kuthetsa kuipitsidwa komwe kumachitika chifukwa cha zinthu za PCR ndikuwonetsetsa kutsimikizika ndi kulondola kwa kukulitsa.
Kayendedwe kantchito
Zosungirako
1.Mayendedwe
Njira yonse yoyendetsa mabokosi otsika kutentha kwa ayezi, kuonetsetsa kuti zida Gawo II zili m'malo a <4 ℃.
2.Kusungirako
Gawo I la chida ichi liyenera kusungidwa kutentha kapena kutentha kwa 2-8 ° C.
Gawo II liyenera kusungidwa pa -20 ° C.