• facebook
  • linkedin
  • youtube
tsamba_banner

Bzalani Mbeu (yachikulu ndi yapakatikati) Direct PCR Plus Kit II-UNG(popanda Zida Zoyeserera)

Kufotokozera Kwa Zida:

Mwachindunji ntchito polysaccharide polyphenol mbewu lysate monga template kwa PCR matalikidwe, popanda m'zigawo osiyana DNA, kudya ndi mkulu tilinazo.Dongosolo la UNG loletsa kuwononga chilengedwe lawonjezedwa kuti tipewe zolakwika.

Palibe kuyeretsa kwa DNA kotenga nthawi komanso kokwera mtengo komwe kumafunikira.

Zofuna zachitsanzo ndizochepa, ingotenga mbeu imodzi.

Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo ntchitoyo ndi yosavuta.

Makina okhathamiritsa a PCR amathandizira PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.

mphamvu zam'tsogolo


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Kufotokozera

Mankhwalawa amagwiritsa ntchito njira yapadera ya lysis reaction, yomwe imatha kumasula mwamsanga DNA ya genomic kuchokera ku zitsanzo za mbeu zokhala ndi ma polysaccharides apamwamba ndi ma polyphenols (monga nthochi, thonje, ndi zina zotero) kuti zigwiritsidwe ntchito pazochitika za PCR, choncho ndizoyenera kwambiri kuyesa chibadwa chachikulu.Pofuna kukwaniritsa zofunikira za mayeso osiyanasiyana ozindikira, zida izi zitha kugwiritsa ntchito mitundu ingapo ya zitsanzo (monga njere zonse, zodula ting'onoting'ono, kapena zitsanzo zanthaka zingapo) poyesera.Pakati pawo, kwa zitsanzo za mbewu zomwe zimayenera kumera, mutha kusankha kudula zidutswa zazing'onoting'ono (1-5mg) kupatula miluza ya mbewu yoyesera;pazoyeserera zomwe zimafunikira kuyesa ndikuyesa zitsanzo zambiri, mutha kusankha kusakaniza zitsanzo zingapo ndikuziphatikiza.Pambuyo pogaya mu tinthu tating'onoting'ono ta 0.5mm, kuyesako kutha kuchitika (osachepera 0.1% ya chitsanzo chandamale amatha kuzindikirika).

Zofotokozera

50T,200T,500T,2000T

Zida zamagulu

Plant Seed Direct PCR Plus Kit II-UNG

Polysaccharide polyphenol mbewu mbewu (yaikulu ndi yapakatikati) mwachindunji PCR kit-UNG

 Kupanga kwa zida

Mtengo wa TP-0318T Mtengo wa TP-03181

Mtengo wa TP-03182

Mtengo wa TP-03183
50 T 200 T 500 T 2000 T
 

Gawo I

Mtengo wa SSP1 24ml ku 96ml pa

120ml × 2

480ml × 2
Mtengo SP2 3ml ku 10 ml pa 25ml ku 100 ml
6 × DNA Loading Buffer 1.5ml pa 1.5ml pa 1.5ml pa 1.5ml × 4
 

Gawo II

Mtengo wa SSP2 8ml ku 32ml ku 80ml ku 80ml 4
2× Mbewu PCR EasyTM Mix 500μl 1mlx2 pa

1.7ml × 3

1.7ml × 12
Pamanja 1 1 1 1

Mbali ndi ubwino

- Palibe kuyeretsa nthawi komanso mtengo wa DNA.

- Zofuna zachitsanzo ndizochepa, ingotengani mbeu imodzi.

- Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo ntchitoyo ndi yosavuta.

- Makina okhathamiritsa a PCR amathandizira PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.

- Anti-kuipitsa PCR dongosolo 2 × Mbewu PCR EasyTM Mix (UNG), yomwe imatha kuthetsa kuipitsidwa komwe kumachitika chifukwa cha zinthu za PCR ndikuwonetsetsa kutsimikizika ndi kulondola kwa kukulitsa.

Kayendedwe kantchito

Plant Seed Direct PCR Plus Kit I(Yaing'ono)-UNG

Zosungirako

1.Mayendedwe

Njira yonse yoyendetsa mabokosi otsika kutentha kwa ayezi, kuonetsetsa kuti zida Gawo II zili m'malo a <4 ℃.

2.Kusungirako

Gawo I la chida ichi liyenera kusungidwa kutentha kapena kutentha kwa 2-8 ° C.

Gawo II liyenera kusungidwa pa -20 ° C.


  • Zam'mbuyo:
  • Ena:

  • Lembani uthenga wanu apa ndikutumiza kwa ife