China Chomera Mbewu(Polysaccharide Polyphenol rich Small) Direct PCR Plus Kit I (popanda Zitsanzo Zida) Wopanga ndi Wopereka |Foregene

Mbewu Zomera(Polysaccharide Polyphenol Rich Small) Direct PCR Plus Kit I (popanda Zida Zoyeserera)

Mbewu Zomera(Polysaccharide Polyphenol Rich Small) Direct PCR Plus Kit I (popanda Zitsanzo Zida) Chithunzi Chowonetsedwa

Kufotokozera Kwa Zida:

Mwachindunji ntchito polysaccharide polyphenol mbewu lysate monga template kwa PCR matalikidwe, popanda m'zigawo osiyana DNA, kudya ndi mkulu tilinazo.

◮Palibe kuyeretsa kwa DNA kotenga nthawi komanso kokwera mtengo komwe kumafunikira.

◮Zofuna zachitsanzo ndizochepa, ingotenga mbeu imodzi.

◮Palibe mankhwala apadera monga kugaya ndi kuphwanya omwe amafunikira, ndipo ntchitoyo ndi yosavuta.

◮Makina okhathamiritsa a PCR amathandizira PCR kukhala ndi mawonekedwe apamwamba komanso kulolerana mwamphamvu kwa PCR reaction inhibitors.

Titumizireni imelo Tsitsani ngati PDF

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Kufotokozera

Mankhwalawa amagwiritsa ntchito njira yapadera ya lysis reaction, yomwe imatha kumasula mwamsanga DNA ya genomic kuchokera ku zitsanzo za mbeu zokhala ndi ma polysaccharides apamwamba ndi ma polyphenols (monga nthochi, thonje, ndi zina zotero) kuti zigwiritsidwe ntchito pazochitika za PCR, choncho ndizoyenera kwambiri kuyesa chibadwa chachikulu.Pofuna kukwaniritsa zofunikira za mayeso osiyanasiyana ozindikira, zida izi zitha kugwiritsa ntchito mitundu ingapo ya zitsanzo (monga njere zonse, zodula ting'onoting'ono, kapena zitsanzo zanthaka zingapo) poyesera.Pakati pawo, kwa zitsanzo za mbewu zomwe zimayenera kumera, mutha kusankha kudula zidutswa zazing'onoting'ono (1-5mg) kupatula miluza ya mbewu yoyesera;pazoyeserera zomwe zimafunikira kuyesa ndikuyesa zitsanzo zambiri, mutha kusankha kusakaniza zitsanzo zingapo ndikuziphatikiza.Pambuyo pogaya mu tinthu tating'onoting'ono ta 0.5mm, kuyesako kutha kuchitika (osachepera 0.1% ya chitsanzo chandamale amatha kuzindikirika).

Zofotokozera

50T,200T,500T,2000T

Zida zamagulu

Plant Seed Direct PCR Plus Kit I Polysaccharide Polyphenol Plant Seed (Yaing'ono) Direct PCR Kit
Kupanga kwa zida		Chithunzi cha TP-0315T	Mtengo wa TP-03151	Mtengo wa TP-03152	Mtengo wa TP-03153
Kupanga kwa zida		50 T	200 T	500 T	2000 T
Gawo I	Mtengo wa SSP1	4.5ml pa	18ml ku	45ml ku	90ml × 2
	Mtengo SP2	3ml ku	10 ml pa	25ml ku	100 ml
	6 × DNA Loading Buffer	1.5ml pa	1.5ml pa	1.5ml pa	1.5ml × 4
Gawo II	Mtengo wa SSP2	1.5ml pa	6ml ku	15ml ku	60 ml pa
Gawo II	2 × Mbewu PCR Yosavuta^TMSakanizani	500μl	1mlx2 pa	1.7ml × 3	1.7ml × 12
Pamanja		1	1	1	1