Plant Total RNA Isolation Plus Kit
Kufotokozera Kwa Zida:
Chotsani bwino DNA pogwiritsa ntchito DNA-Cleaning Column
Chotsani DNA popanda kuwonjezera DNase
Zosavuta-zochita zonse zimatsirizidwa pa kutentha kwa chipinda
Kuthamanga-ntchito ikhoza kutha m'mphindi 30
Otetezeka-palibe organic reagent yomwe imagwiritsidwa ntchito
Zofotokozera
50 Preps, 200 Preps
Chidacho chimagwiritsa ntchito spin column ndi formula yopangidwa ndi Foregene, yomwe imatha kuchotsa bwino RNA yoyera komanso yapamwamba kwambiri kuchokera kumitundu yosiyanasiyana yazitsamba yokhala ndi ma polysaccharides apamwamba kapena ma polyphenols.Amapereka DNA-Cleaning column yomwe imatha kuchotsa mosavuta DNA ya genomic kuchokera ku supernatant ndi minofu lysate.Mzere wa RNA wokha ukhoza kumangirira RNA.Chidacho chimatha kukonza zitsanzo zambiri nthawi imodzi.
Dongosolo lonse lilibe RNase, kotero RNA yoyeretsedwa sidzanyozedwa.Buffer PRW1 ndi Buffer PRW2 akhoza kuonetsetsa kuti RNA yopezedwa siipitsidwa ndi mapuloteni, DNA, ayoni, ndi organic compounds.
Zida zamagulu
| Buffer PSL1, Buffer PS, Buffer PSL2 |
| Buffer PRW1, Buffer PRW2 |
| RNase-Free ddH2O, Gawo Loyeretsa DNA |
| RNA-Only Column |
Mbali&zabwino
■ Opaleshoni firiji (15-25 ℃) lonse ndondomeko, popanda ayezi kusamba ndi otsika kutentha centrifugation.
■ Zida zonse za RNase-Free, palibe chifukwa chodera nkhawa za kuwonongeka kwa RNA.
■ Oyenera makamaka kuyeretsedwa kwa RNA kuchokera ku zitsanzo za zomera za polysaccharides ndi polyphenols.
■ DNA-Cleaning Column imamangiriza ku DNA, kuti zida zitha kuchotsa kuipitsidwa kwa DNA popanda kuwonjezera DNase.
■ Zokolola zambiri za RNA: Mzere wa RNA-okha ndi fomula yapadera imatha kuyeretsa RNA bwino.
■ Kuthamanga kwachangu: kosavuta kugwiritsa ntchito ndipo kumatha kutha mkati mwa mphindi 30.
■ Chitetezo: palibe organic reagent yomwe imafunikira.
■ Ubwino wapamwamba: Zidutswa za RNA zoyeretsedwa ndi zoyera kwambiri, zopanda zomanga thupi ndi zonyansa zina, ndipo zimatha kukwaniritsa ntchito zosiyanasiyana zoyesera kumunsi kwa mtsinje.
Mankhwala magawo
■ Ntchito zotsika: kaphatikizidwe ka cDNA yoyamba, RT-PCR, kupanga maselo, Northern Blot, etc.
■ Zitsanzo: Zomera zatsopano kapena zachisanu za polysaccharides ndi polyphenols
■ Mlingo: 50mg chomera minofu
■ Kuchuluka kwa RNA kumangiriza gawo loyeretsa: 80 μg
■ Voliyumu yowonjezera: 50-200 μl
Kit application
Ndiwoyenera kuchotsa ndi kuyeretsa RNA yonse kuchokera ku zitsanzo zamafuta a zomera zatsopano kapena zachisanu (makamaka masamba atsopano a masamba) okhala ndi polysaccharide ndi polyphenol.
Kuyenda kwa ntchito
Chithunzi
Plant Total RNA Isolation Kit Plus inakonzedwa 50mg masamba atsopano a polysaccharides ndi polyphenols, ndipo 5% yoyeretsedwa RNA inayesedwa ndi electrophoresis.
1: nthochi
2: Chiko
3: thonje
4: Makangaza
Kusungirako ndi moyo wa alumali
Zidazi zitha kusungidwa kwa miyezi 24 pansi pamikhalidwe yowuma kutentha (15-25 ℃);Ngati ikufunika kusungidwa kwa nthawi yayitali, imatha kusungidwa mu 2-8 ℃.
Buffer PSL1 ikhoza kuyikidwa pa 4℃ kwa mwezi umodzi mutawonjezera β-mercaptoethanol (ndikofunikira kuti muwonjezere nthawi yomweyo yoyesera).
