Plant Total RNA Isolation Kit

Kufotokozera Kwa Zida:

RNase-Free

Chotsani bwino DNA pogwiritsa ntchito DNA-Cleaning Column

Chotsani DNA popanda kuwonjezera DNase

Zosavuta-zochita zonse zimatsirizidwa pa kutentha kwa chipinda

Kuthamanga-ntchito ikhoza kutha m'mphindi 30

Otetezeka-palibe organic reagent yomwe imagwiritsidwa ntchito

foregene strength


Tsatanetsatane wa Zamalonda

Zogulitsa Tags

Zofotokozera

50 Preps, 200 Preps

Chidacho chimagwiritsa ntchito spin column ndi formula yopangidwa ndi Foregene, yomwe imatha kuchotsa bwino RNA yapamwamba komanso yapamwamba kwambiri kuchokera kumitundu yosiyanasiyana yazitsamba yokhala ndi ma polysaccharides otsika ndi ma polyphenols.Pazitsanzo za zomera zokhala ndi ma polysaccharides apamwamba kapena ma polyphenols, tikulimbikitsidwa kugwiritsa ntchito Plant Total RNA Isolation Plus Kit kuti mupeze zotsatira zabwinoko zochotsa RNA.Chidachi chimapereka DNA-Kuyeretsa ndime yomwe imatha kuchotsa mosavuta DNA ya genomic kuchokera ku supernatant ndi minofu lysate.Mzere wa RNA wokha ukhoza kumangirira RNA.Chidacho chimatha kukonza zitsanzo zambiri nthawi imodzi.

Dongosolo lonse lilibe RNase, kotero RNA yoyeretsedwa sidzanyozedwa.Buffer PRW1 ndi Buffer PRW2 akhoza kuonetsetsa kuti RNA yopezedwa siipitsidwa ndi mapuloteni, DNA, ayoni, ndi organic compounds.

Zida zamagulu

Buffer PSL1, Buffer PS, Buffer PSL2

Buffer PRW1, Buffer PRW2

RNase-Free ddH2O, Gawo Loyeretsa DNA

RNA-Only Column

Malangizo

Mbali&zabwino

■ Opaleshoni firiji (15-25 ℃) lonse ndondomeko, popanda ayezi kusamba ndi otsika kutentha centrifugation.
■ Zida zonse za RNase-Free, palibe chifukwa chodera nkhawa za kuwonongeka kwa RNA.
■ DNA-Cleaning Column imamangiriza ku DNA, kuti zida zitha kuchotsa kuipitsidwa kwa DNA popanda kuwonjezera DNase.
■ Zokolola zambiri za RNA: Mzere wa RNA-okha ndi fomula yapadera imatha kuyeretsa RNA bwino.
■ Kuthamanga kwachangu: kosavuta kugwiritsa ntchito ndipo kumatha kutha mkati mwa mphindi 30.
■ Chitetezo: palibe organic reagent yomwe imafunikira.
■ Ubwino wapamwamba: Zidutswa za RNA zoyeretsedwa ndi zoyera kwambiri, zopanda zomanga thupi ndi zonyansa zina, ndipo zimatha kukwaniritsa ntchito zosiyanasiyana zoyesera kumunsi kwa mtsinje.

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Kit application

Ndiwoyenera kuchotsa ndi kuyeretsa RNA yonse kuchokera ku zitsanzo zamtundu wa zomera zatsopano kapena zachisanu (makamaka masamba atsopano a masamba) okhala ndi polysaccharide yochepa ndi polyphenol.

Kuyenda kwa ntchito

plant total RNA-simple workflow

Chithunzi

Plant Total RNA Isolation Kit6

Plant Total RNA Isolation Kit Plus inakonzedwa 50mg masamba atsopano a polysaccharides ndi polyphenols, ndipo 5% yoyeretsedwa RNA inayesedwa ndi electrophoresis.
1: nthochi
2: Chiko
3: thonje
4: Makangaza

Kusungirako ndi moyo wa alumali

Zida zimatha kusungidwa kwa miyezi 12 kutentha kwa firiji (15-25 ℃) pamalo owuma, ndi 2-8 ℃ kwa nthawi yayitali (miyezi 24).

Buffer PSL1 ikhoza kusungidwa pa 4 ℃ kwa mwezi umodzi mutawonjezera 2-hydroxy-1-ethanethiol(ngati mukufuna).


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