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Bzalani Chiwerengero cha RNA Chokha
Chikwamachi chimagwiritsa ntchito gawo loyambira ndi chilinganizo chopangidwa ndi Foregene, chomwe chimatha kuchotsa kuyera kwambiri komanso kwapamwamba kwambiri kwa RNA kuchokera kumatumba osiyanasiyana okhala ndi polysaccharides otsika ndi polyphenols. Pazitsanzo zazomera zomwe zili ndi polysaccharides kapena polyphenols, tikulimbikitsidwa kuti mugwiritse ntchito Plant Total RNA Isolate Plus Kit kuti mupeze zotsatira zabwino za RNA. Chikwamacho chimapereka gawo loyera la DNA lomwe lingachotse mosavuta genomic DNA kuchokera ku supernatant ndi minofu lysate. Mzere wa RNA wokha umatha kumangiriza RNA. The zida akhoza pokonza ambiri zitsanzo pa nthawi yomweyo.
Makina onsewa alibe RNase, chifukwa chake RNA yoyeretsedwa sidzakhala yowonongeka. Buffer PRW1 ndi Buffer PRW2 zitha kuonetsetsa kuti RNA yomwe idapezedwa siyodetsedwa ndi mapuloteni, DNA, ayoni, ndi zinthu zina.
