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Bzalani tsamba la Direct PCR Plus
Chogulitsachi chimagwiritsa ntchito makina apadera a lysis pofunafuna masamba obzala. Lysate itha kugwiritsidwa ntchito ngati template popanda kuyeretsa. Pambuyo powonjezera zoyambira, PCR Mix ya chida ichi itha kugwiritsidwa ntchito kukulitsa.
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Bzalani Mbewu Mwachindunji PCR Kit I / II
Chida ichi chimagwiritsa ntchito njira yapadera ya lysis pofesa mbewu. Lysate itha kugwiritsidwa ntchito ngati template popanda kuyeretsa. Pambuyo powonjezera zoyambira, PCR Mix ya chida ichi itha kugwiritsidwa ntchito kukulitsa.
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Bzalani tsamba Direct PCR kuphatikiza zida-UNG
Kutengera chida cha Plant Leaf Direct PCR Plus, dUTP imagwiritsidwa ntchito m'malo mwa dTTP, ndipo enzyme ya UNG yomwe imatha kutsitsa template yomwe ili ndi dUTP imawonjezeredwa. Mwanjira imeneyi, kutsimikizika ndi kulondola kwa kukulitsa kumatsimikiziridwa, ndipo vuto la kuipitsidwa kwa mankhwala a PCR lomwe lingachitike pakuyesa kwamitundu yayikulu limalephereka.
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Bzalani tsamba Direct PCR kit-UNG
Kutengera chida cha Plant Leaf Direct PCR, dUTP imagwiritsidwa ntchito m'malo mwa dTTP, ndipo enzyme ya UNG yomwe imatha kutsitsa template yomwe ili ndi dUTP imawonjezeredwa. Mwanjira imeneyi, kutsimikizika ndi kulondola kwa kukulitsa kumatsimikiziridwa, ndipo vuto la kuipitsidwa kwa mankhwala a PCR lomwe lingachitike pakuyesa kwamitundu yayikulu limalephereka.
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Bzalani tsamba lachindunji la PCR
Chogulitsachi chimagwiritsa ntchito makina apadera a lysis pofunafuna masamba obzala. Lysate itha kugwiritsidwa ntchito ngati template popanda kuyeretsa. Zoyambira zitha kuwonjezedwa ndipo PCR Mix ya chida ichi itha kugwiritsidwa ntchito kukulitsa.
