Direct PCR

Kodi Direct PCR ndi chiyani

Direct PCR ndi njira yokulitsa DNA molunjika kuchokeramagazi, minofu ya nyama,makoswe mchira,selo, khutu la khoswe,mbidzi nsomba, mazira a nsomba,masamba masamba,mbewukapena zitsanzo zina zoyambira zachilengedwe popanda kupanga DNA kudzipatula ndikuyeretsa.Njira ya Direct PCR ikhoza kuchepetsa kwambiri nthawi yoyesera, komanso mtengo wa genotyping ndi ma projekiti apamwamba kwambiri.Imaperekanso njira yabwinoko mukakumana ndi zovuta zokulitsa zitsanzo zazing'ono kwambiri pomwe gawo loyeretsera limatha kuyambitsa kutayika kwachitsanzo.

FOREGENE's PCR Mix ili ndi mphamvu yolimbana ndi kupsinjika, chifukwa cha FOREGENE mphamvu yovomerezeka ya Taq enzyme( patent yovomerezeka: ZL20161034512.0 (China), EP3450560 (Europe), PCT/CN2017/082154 (US), patent 6894926 (Japan)).Taq enzyme yake imagwiritsa ntchito ukadaulo wa DNA recombination kuti agwirizanenso chigawo chomangirira cha DNA cha ma archaeal nucleic acid omwe amamanga mapuloteni okhala ndi Taq DNA polymerase osamva kutentha kuti apange template ya DNA yokhala ndi kuyanjana kowonjezereka.Taq yotentha yoyambira bwino yomwe imasungabe ntchito zosiyanasiyana za DNA polymerase yoyambirira. Taq enzyme iyi imatha kumangirira ku template ya DNA ndikuyambitsa kaphatikizidwe ka DNA m'ma tempulo otsika komanso malo ovuta.

For chitsanzo,itndizosavuta monga kuwonjezera zanucell,chomera (tsamba laling'ono, muzu kapena njere) kapena zitsanzo za nyama kubuffer, ndikuwonjezera wapaderamaster sakanizani ndimwachindunjizoyambira mu machubu a PCR ndikuyendetsa kudzera pa chozungulira chotenthetsera.Kuchita bwino kwa zida ndikwabwino pakuwunika zitsanzo zazikulu pomwe kupulumutsa nthawi ndikofunikira kwambiri.

Makina a PCR omwe ali ndi UNG enzyme ndi dUTP amasankhidwa malinga ndi zoyeserera, zomwe zimatha kupewa kuipitsidwa komwe kumachitika chifukwa cha zinthu zokulitsa za PCR.

asdada (5)
asdada (1)

Ubwino waDPCR yabwino

 

 

Direct PCR

Njira yachikhalidwe

(Kuyeretsa kwa RNA+ PCR yachizolowezi)

Kugwiritsa ntchito zinthu

Kudya minofu

Zochepa

Zambiri

Kukonzekera ma template

Mtundu wazinthu

Palibe akupera chofunika

Akupera zitsanzo

Ntchito

Mtundu umodzi chubu, 10min

ntchito yovuta, 1h

Flux

1-96

1-24

Zotsatira za PCR

kachitidwe kachitidwe

Kusakaniza kwa 2 × PCR

Pa, MgCl2, 10 × PCR Buffer, Taq enzyme,

Akupempha

01 Kuzindikiritsa tizilombo toyambitsa matenda

02 Chizindikiritso chosinthidwa ma genetic, genotyping

03 Multiplex PCR / SNP kuzindikira / PCR-RFLP

04Kutsata / kufananiza

Product Series

 

*Covid Nucleic acid RT-PCR kit, ya ORF1ab, N, E,S, ndi masinthidwe ena, monga mtundu wa Delta, etc.

96Rxns/kit

Ubwino:

-Malizani mayesowo pakangotha ​​ola limodzi

-Palibe chifukwa chochotsa RNA ndikudzipatula

-Kukhazikika kwapamwamba komanso tcheru

Tsatanetsatane wa zida zoyeserera za Covid Dinani apa

 

 

 

asdada (3)

Mndandanda

Dzina lazogulitsa

Zofotokozera

Nambala ya Catalog

Zosungirako

Direct RT-qPCR Series

QuickEasyTMCell Direct RT-qPCR Kit-SYBR Green I

200T

DRT-01011

Gawo I 4 ℃, Gawo II -20 ℃

1000T

DRT-01012

asdada (12)

 

Mndandanda

Dzina lazogulitsa

Zofotokozera

Nambala ya Catalog

Zosungirako

 

 

 

Magazi Direct PCR Series

Magazi SuperDirectTMPCR Kit (EDTA)

200 × 20μl rxns

Mtengo wa TP-04111

-20 ℃

2000 × 20μl rxns

Mtengo wa TP-04113

Magazi SuperDirectTMPCR Kit (EDTA) -UNG

200 × 20μl rxns

Mtengo wa TP-04121

-20 ℃

2000 × 20μl rxns

Mtengo wa TP-04123

Magazi SuperDirectTMChithunzi cha PCR
(Heparin)

200 × 20μl rxns

Mtengo wa TP-04211

-20 ℃

2000 × 20μl rxns

Mtengo wa TP-04213

Magazi SuperDirectTMPCR Kit (Heparin) -UNG

200 × 20μl rxns

Mtengo wa TP-04221

-20 ℃

2000 × 20μl rxns

Mtengo wa TP-04223

 

asdada (7)
asdada (4)

 

Sizi

Dzina lazogulitsa

Zofotokozera

Nambala ya Catalog

Zosungirako

Animal Direct PCR Series

Zinyama Zanyama Direct PCR Kit(65 ℃ lysis)

200 × 20μl rxns

Chithunzi cha TP-01111

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-01113

Zinyama Zanyama Direct PCR Kit(65 ℃ lysis) -UNG

200 × 20μl rxns

Mtengo wa TP-01121

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-01123

Mouse Mchira SuperDirectTMChithunzi cha PCR
(kutentha kwa chipinda)

200 × 20μl rxns

Mtengo wa TP-01311

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-01313

Mouse Tail Direct PCR Kit (65 ℃ lysis)

200 × 20μl rxns

Mtengo wa TP-01331

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-01333

Mouse Tail Direct PCR Kit(65 ℃ lysis) - UNG

200 × 20μl rxns

Mtengo wa TP-01341

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-01343

Zebra Fish Direct PCR Kit (65 ℃ lysis)

200 × 20μl rxns

Mtengo wa TP-01411

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Chithunzi cha TP-01413

Zebra Fish Direct PCR Kit(65 ℃ lysis) - UNG

200 × 20μl rxns

Mtengo wa TP-01421

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-01423

asdada (8)
asdada (10)
asdada (9)
asdada (11)

Mndandanda

Dzina lazogulitsa

Zofotokozera

Nambala ya Catalog

Zosungirako

Bzalani Direct PCR Series

Plant Leaf Direct PCR Kit

200 × 20μl rxns

Mtengo wa TP-02111

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-02113

Plant Leaf Direct PCR Kit -UNG

200 × 20μl rxns

Mtengo wa TP-02121

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-02123

Plant Leaf Direct PCR Plus Kit
(Polysaccharide polyphenol chomera)

200 × 20μl rxns

Mtengo wa TP-02131

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-02133

Plant Leaf Direct PCR Plus Kit-UNG
(Polysaccharide polyphenol chomera)

200 × 20μl rxns

Mtengo wa TP-02141

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-02143

Plant Seed Direct PCR Kit I

200 × 20μl rxns

Mtengo wa TP-03111

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-03113

Plant Seed Direct PCR Kit I -UNG

200 × 20μl rxns

Mtengo wa TP-03121

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-03123

Bzalani Mbewu Direct PCR Kit II

200 × 20μl rxns

Mtengo wa TP-03131

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-03133

Plant Seed Direct PCR Kit II -UNG

200 × 20μl rxns

Mtengo wa TP-03141

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-03143

Plant Seed Direct PCR Plus Kit I(Polysaccharide polyphenol plant)

200 × 20μl rxns

Mtengo wa TP-03151

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-03153

Plant Seed Direct PCR Plus Kit I - UNG(Polysaccharide polyphenol plant)

200 × 20μl rxns

Mtengo wa TP-03161

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-03163

Bzalani Mbewu Direct PCR Plus Kit II

200 × 20μl rxns

Mtengo wa TP-03171

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-03173

Bzalani Mbewu Direct PCR Plus Kit II - UNG(Polysaccharide polyphenol plant)

200 × 20μl rxns

Mtengo wa TP-03181

Gawo I 4 ℃, Gawo II -20 ℃

2000 × 20μl rxns

Mtengo wa TP-03183