Kodi Direct PCR ndi chiyani

Direct PCR ndi njira yokulitsa DNA molunjika kuchokeramagazi, minofu ya nyama,makoswe mchira,selo, khutu la khoswe,mbidzi nsomba, mazira a nsomba,masamba masamba,mbewukapena zitsanzo zina zoyambira zachilengedwe popanda kupanga DNA kudzipatula ndikuyeretsa.Njira ya Direct PCR ingachepetse kwambiri nthawi yoyesera, komanso mtengo wa genotyping ndi mapulojekiti okwera kwambiri.Imaperekanso njira yabwinoko mukakumana ndi zovuta zokulitsa zitsanzo zazing'ono kwambiri pomwe gawo loyeretsera limatha kuyambitsa kutayika kwachitsanzo.

FOREGENE's PCR Mix ili ndi mphamvu yolimbana ndi kupsinjika, chifukwa cha FOREGENE mphamvu yovomerezeka ya Taq enzyme( patent yovomerezeka: ZL20161034512.0 (China), EP3450560 (Europe), PCT/CN2017/082154 (US), patent 6894926 (Japan)).Taq enzyme yake imagwiritsa ntchito ukadaulo wa DNA recombination kuti uphatikizenso chigawo chomangirira cha DNA cha ma archaeal nucleic acid omwe amamanga mapuloteni okhala ndi Taq DNA polymerase osamva kutentha kuti apange template ya DNA yokhala ndi kuyanjana kowonjezereka.Taq yotentha yoyambira bwino yomwe imasungabe ntchito zosiyanasiyana za DNA polymerase. Izi Taq enzyme imatha kumangirira ku template ya DNA ndikuyambitsa kaphatikizidwe ka DNA m'ma tempulo otsika komanso malo ovuta.

For chitsanzo,itndizosavuta monga kuwonjezera zanucell,chomera (tsamba laling'ono, muzu kapena njere) kapena zitsanzo za nyama kubuffer, ndikuwonjezera wapaderamaster sakanizani ndimwachindunjizoyambira mu machubu a PCR ndikuyendetsa kudzera pa chozungulira chotenthetsera.Kuchita bwino kwa zida ndikwabwino pakuwunika zitsanzo zazikulu pomwe kupulumutsa nthawi ndikofunikira kwambiri.

Dongosolo la PCR lomwe lili ndi UNG enzyme ndi dUTP limasankhidwa molingana ndi zofunikira zoyeserera, zomwe zitha kupewa kuipitsidwa komwe kumayambitsidwa ndi zinthu zokulitsa za PCR.