RT-PCR ndiukadaulo womwe umaphatikizira reverse transcript RT ya RNA ndi polymerase chain amplification (PCR) ya cDNA.Ukadaulo wa RT-PCR ndiwodziwikiratu komanso wosunthika, ndipo ungagwiritsidwe ntchito kuzindikira kuchuluka kwa mawonekedwe a jini m'maselo/minofu, zomwe zili m'ma virus a RNA m'maselo, komanso kuphatikizika kwachindunji kwa magawo a cDNA amtundu wina.
ZABWINO KWAMBIRI:
✱ RT Kit ya Foregene imakhala yokhazikika pa kutentha kwa 50-65 ℃ (65°C ikhoza kutsegula mulingo wachiwiri wa RNA kuti zolembedwazo ziyende bwino)
✱ Kulekerera kwamphamvu, kumatha kupirira mchere wina wa ethanol ndi guanidine
| Sub-Mndandanda | Dzina lazogulitsa | Zofotokozera | Nambala ya Catalog | Zosungirako |
| PCR Mix Series | 5ml ku | Mtengo wa PH-01013 | -20 ℃ | |
| 40 ml pa | Mtengo wa PH-01015 | |||
| 5ml ku | Chithunzi cha PH-01013-B | -20 ℃ | ||
| 40 ml pa | Chithunzi cha PH-01015-B | |||
| 1 ml | DP-20031 | -20 ℃ | ||
| 3 × 1.7ml | DP-20032 | |||
| 3 × 1.7ml | Chithunzi cha DP-20032-B | -20 ℃ | ||
| 50 ml pa | Chithunzi cha DP-20034-B | |||
| 2 × Rapid PCR reaction premix system 2 × PCR Mix | 1 ml | DP-20041 | -20 ℃ | |
| 3 × 1.7ml | DP-20042 | |||
| 500 × 20μl rxns | QP-01012 | -20 ℃ | ||
| 2000 × 20μl rxns | QP-01014 | |||
| 500 × 20μl rxns | QP-01022 | -20 ℃ | ||
| 2000 × 20μl rxns | QP-01024 | |||
| Chithunzi cha RT | 25 × 20μl Zochita | Mtengo wa RT-01011 | -20 ℃ | |
| 100 × 20μl Kuchita | Mtengo wa RT-01012 | |||
| (Master Premix for first-strand cDNA synthesis for Real Time PCR) | 200 × 10μl Kuchita | Mtengo wa RT-01022 | -20 ℃ | |
| 800 × 10μl Kuchita | Mtengo wa RT-01023 | |||
| Master Premix for first-strand cDNA synthesis for Real Time PCR yokhala ndi gDNase | 100 × 20μl Kuchita | Mtengo wa RT-01032 | -20 ℃ | |
| Lnc-RT HeroTM Ine(Ndi gDNase)(Super Premix for first-strand cDNA synthesis from lncRNA) | 25 × 20μl Kuchita | RTL-09098 | -20 ℃ | |
| 50 × 20μl Kuchita | RTL-09099 | |||
| RT-PCR mndandanda | 100 rxn | RT-02011 | -20 ℃ | |
| 500 rxn | RT-02012 | |||
| 100 rxn | RT-02021 | -20 ℃ | ||
| 500 rxn | RT-02022 | |||
| RT-qPCR mndandanda | 100 rxn | Mtengo wa RT-02111 | -20 ℃ | |
| 500 rxn | Mtengo wa RT-02112 | |||
| 100 rxn | Mtengo wa RT-02131 | -20 ℃ | ||
| 500 rxn | Mtengo wa RT-02132 | |||
| DNA Marker | 50 Zokonzekera | Chithunzi cha DL-20011 | 4 ℃ | |
| 200 Preps | Chithunzi cha DL-20012 | |||
| 50 Zokonzekera | Mtengo wa DL-30011 | 4 ℃ | ||
| 200 Preps | Mtengo wa DL-30012 |
