Real Time PCR Easyᵀᴹ-SYBR Green I kit
Kufotokozera Kwa Zida:
Zosavuta—2X PCR Mix kuti muchepetse zolakwika zoyeserera ndi nthawi yogwira ntchito
Specific-optimized buffer ndi hot-start Taq enzyme imatha kuteteza kukulitsa kosakhazikika komanso mapangidwe a dimer.
Kumverera kwakukulu - kumatha kuzindikira makope otsika a template
Kusinthasintha kwabwino - kumagwirizana ndi zida zenizeni zenizeni za PCR
Mafotokozedwe a Kit
The 2 × Real PCR YosavutaTMMix-SYBR yoperekedwa ndi Real Time PCR EasyTM-SYBR Green I kit ndi dongosolo latsopano la premix lomwe limagwiritsa ntchito SYBR Green I for Real Time PCR amplification reactions, yomwe ingathe kusintha kwambiri tsatanetsatane wa malonda ndi kachitidwe kake.ROX imaperekedwa ngati utoto wowongolera mkati.Kuchuluka kwa fluorescence kwa zida izi ndi 3-5 kuwirikiza kawiri kwa zinthu zofanana, zomwe zimatha kukhala zovutirapo komanso kuwonetsa mwachilengedwe kuchuluka kwa template ya DNA.
2 × Real PCR YosavutaTMMix-SYBR ili ndi Taq DNA Polymerase yapadera ya Foregene.Poyerekeza ndi ma enzyme wamba a Taq, ili ndi zabwino zake pakukweza kwakukulu, kuthekera kokulirapo kwamphamvu komanso kutsika kosagwirizana.Itha kuchepetsa kukulitsa kosakhazikika ndikuwongolera kulondola kwa PCR.
Zofotokozera
| Real Time PCR Easyᵀᴹ-SYBR Green I Kit | ||||
| Kapangidwe ka zida (20μl system) | QP-01011 | QP-01012 | QP-01013 | QP-01014 |
| 200T | 500T | 1000T | 2000T | |
| 2 × Real PCR YosavutaTMMix-SYBR | 1 ml × 2 | 1.7ml × 3 | 1.7ml × 6 | 1.7ml × 12 |
| 50 × ROX Reference Dye | 400 μl | 1 ml | 1 ml × 2 | 1 ml × 4 |
| DNase-Free ddH2O | 1.7 ml | 1.7ml × 2 | 10 ml | 20 ml |
| Malangizo | 1 | 1 | 1 | 1 |
Mbali & ubwino
■ Yosavuta—2X PCR Mix kuti muchepetse zolakwika zoyeserera ndi nthawi yogwira ntchito
■ Specific-optimized buffer ndi hot-start Taq enzyme imatha kuletsa kukulitsa kosakhazikika komanso kupangika kwa dimer.
■ Kuzindikira kwakukulu-kumatha kuzindikira makope otsika a template
■ Kusinthasintha kwabwino—kumagwirizana ndi zida zambiri zapanthawi yeniyeni za PCR
Kit application
qPCR kusanthula
Kuyenda kwa ntchito
Zithunzi
Kusungirako ndi moyo wa alumali
Chidachi chiyenera kusungidwa kutali ndi kuwala ndipo chiyenera kusungidwa pa -20 ℃.Ngati imagwiritsidwa ntchito pafupipafupi, imathanso kusungidwa ku 4 ℃ kwakanthawi kochepa (masiku 10).
