RT Easyᵀᴹ I Master Premix yopanga chingwe choyamba cha cDNA
Kufotokozera Kwa Zida:
- Makina olembera osinthika, zimangotenga mphindi 25 kuti amalize kaphatikizidwe ka chingwe choyamba cha cDNA.
- High-sensitivity reverse transcription system, ma tempulo a pg-level amathanso kupeza cDNA yapamwamba kwambiri.
- Makina osindikizira osinthika amakhala ndi kukhazikika kwamafuta ambiri, kutentha kwamachitidwe kumatha kufika 50 ℃,ndipo ili ndi magwiridwe antchito abwino osinthira.
- Ngakhale zitsanzo zonyansa za RNA (mowa mpaka 45%, mchere wa guanidine mpaka 750mM) ukhoza kusinthidwanso.
Mafotokozedwe a Kit
FOREGENE's 2 × RT YosavutaTMMix imatha kupanga cDNA ya chingwe choyamba pa kutentha kwambiri mpaka 50 ° C, zomwe zimathandizira kuti ma tempuleti a RNA asinthe.
RNA yoyeretsedwa mu labotale nthawi zambiri imakhala ndi mowa ndi zotsalira za mchere wa guanidine, zomwe zimakhala ndi zoletsa zoletsa kwambiri pa reverse transcriptases, zomwe zimapangitsa kuti pakhale zotsatira zosasangalatsa zolembera kapena zolembera.FOREGENE 2 × RT YosavutaTMKusakaniza kumasonyeza kulolerana kwambiri kwa mowa ndi mchere wa guanidine, kulekerera kwapamwamba kwambiri kwa mowa mu zitsanzo za RNA ndi 45%, ndipo kulekerera kwambiri mchere wa guanidine ndi 750mM.Ngakhale RNA ilibe yoyera, zolembera zosinthika zitha kuchitidwa ndi FORREGENE 2 × RT Mix.Dongosolo lapadera la machitidwe limapangitsa kuti machitidwe a RT akhale osavuta, othamanga komanso owoneka bwino, ndipo kaphatikizidwe ka chingwe choyamba cha cDNA kumatha kutha mphindi 25.
Izi zitha kugwiritsidwa ntchito molumikizana ndi Foregene's fulorosenti kuchuluka kwa mankhwala Real Time PCR EasyTMkupeza zotsatira zoyesera zapamwamba.
Zofotokozera
| RT EasyTMIne Master Premix for first-strand cDNA synthesis | ||
| Zamkatimu | Mtengo wa RT-01011 | Mtengo wa RT-01012 |
| 25 nthawi (20μl dongosolo) | 100 nthawi (20μl system) | |
| 2 × RT EasyTM Mix | 0.25 ml | 1 ml |
| Zoyambira Zosasintha (50μM) | 50μl pa | 200μl |
| Oligo(dT)18 Primer (50μM) | 50μl pa | 200μl |
| RNase-Free ddH2O | 1.7ml pa | 1.7ml pa |
| Buku la Malangizo | 1 chidutswa | 1 chidutswa |
Mbali & ubwino
- Makina olembera osinthika, zimangotenga mphindi 25 kuti amalize kaphatikizidwe ka chingwe choyamba cha cDNA.
- High-sensitivity reverse transcription system, ma tempulo a pg-level amathanso kupeza cDNA yapamwamba kwambiri.
- Makina osindikizira osinthika amakhala ndi kukhazikika kwamafuta ambiri, kutentha kwadongosolo kumatha kufika mpaka 50 ℃, ndipo imakhala ndi ntchito yabwino yolembera.
- Ngakhale zitsanzo zonyansa za RNA (mowa mpaka 45%, mchere wa guanidine mpaka 750mM) ukhoza kusinthidwanso.
Kit application
- Ochiritsira RT-PCR
-Kuphatikizika kwa cDNA pakufufuza kwa cloning ndi kufotokozera
- Kuwunika kwa njira yowonjezera yoyambira patsamba loyambira zolembera
- RACE (kukulitsa mwachangu kwa cDNA kumatha)
- Kukulitsa kwa Linear RNA
- Chip chizindikiro
Kuyenda kwa ntchito
Kusungirako ndi moyo wa alumali
1.Mayendedwe
Njira yonse yamayendedwe otsika otsika oundana, kuonetsetsa kuti zida zili m'malo a <4 ℃.
2. Zosungirako
RT EasyTMNdimasungidwa pa -20 ° C.Sungani mankhwalawa mufiriji yokhazikika pa -20 ° C mutangolandira.Ngati zosungirako zili zoyenera, mankhwalawa sangawononge ntchito iliyonse pazaka zovomerezeka za 1.
