RT Easyᵀᴹ II Master Premix ya chingwe choyamba cha cDNA kaphatikizidwe ka Real Time PCR
Kufotokozera Kwa Zida:
- Makina olembera osinthika, zimangotenga mphindi 20 kuti amalize kaphatikizidwe ka chingwe choyamba cha cDNA.
- High-sensitivity reverse transcription system, ma tempulo a pg-level amathanso kupeza cDNA yapamwamba kwambiri.
- Makina osindikizira osinthika amakhala ndi kukhazikika kwamafuta ambiri, kutentha kwadongosolo kumatha kufika mpaka 50 ℃, ndipo imakhala ndi ntchito yabwino yolembera.
- Ngakhale zitsanzo zonyansa za RNA (mowa mpaka 60%, mchere wa guanidine mpaka 750mM) ukhoza kusinthidwanso.
- 2 × RT OR-YosavutaTM Mix yawonjezera zoyambira zosinthira (Random Primer, Oligo(dT)18 Choyamba).
Mafotokozedwe a Kit
Foregene's 2 × RT OR-EasyTMMix imatha kupanga cDNA ya chingwe choyamba pa kutentha kwambiri mpaka 50 ° C, zomwe zimathandizira kuti ma tempuleti a RNA asinthe.
RNA yoyeretsedwa mu labotale nthawi zambiri imakhala ndi mowa ndi zotsalira za mchere wa guanidine, zomwe zimakhala ndi zoletsa zoletsa kwambiri pa reverse transcriptases, zomwe zimapangitsa kuti pakhale zotsatira zosasangalatsa zolembera kapena zolembera.Foregene 2 × RT Mix ikuwonetsa kulolerana kwambiri ndi mowa ndi mchere wa guanidine.Kulekerera kwakukulu kwa mowa mu zitsanzo za RNA ndi 60%, ndipo kulolerana kwambiri kwa mchere wa guanidine ndi 750mM.Ngakhale RNA yodetsedwa itha kugwiritsidwa ntchito posinthira zolemba ndi Foregene 2 × RT OR-EasyTMSakanizani.
2 × RT OR-YosavutaTMMix ndi chosinthira mwachangu chosindikizira chomwe chimapangidwira Real Time PCR.Dongosololi limakhala ndi zolembera zam'mbuyo kwambiri ndipo sizifunikira kuwonjezera zoyambira zilizonse, ndipo zimatha kuchita bwino zolembera pama tempulo ochepa a RNA.Dongosolo lapadera la machitidwe limapangitsa kuti machitidwe a RT akhale osavuta, othamanga komanso abwino kwambiri.Chingwe choyamba cha cDNA kaphatikizidwe amatha kumaliza mphindi 20.Izi zitha kugwiritsidwa ntchito molumikizana ndi Foregene's fulorosenti kuchuluka kwa mankhwala Real Time PCR EasyTMkupeza zotsatira zoyesera zapamwamba.
Zofotokozera
| RT EasyTMII Master Premix for first-strand cDNA synthesis for Real Time PCR | |||
| Zida zamagulu | Mtengo wa RT-01021 | Mtengo wa RT-01022 | Mtengo wa RT-01023 |
| 50 T (10μl dongosolo) | 200 T (10μl dongosolo) | 800 T (10μl dongosolo) | |
| 2 × RT OR-YosavutaTMSakanizani | 0.25 ml | 1 ml | 1ml 4 pa |
| RNase-Free ddH2O | 1.7ml pa | 1.7ml pa | 1.7ml × 3 |
| Buku Lolangiza | 1 chidutswa | 1 chidutswa | 1 chidutswa |
Mbali & ubwino
- Makina olembera osinthika, zimangotenga mphindi 20 kuti amalize kaphatikizidwe ka chingwe choyamba cha cDNA.
- High-sensitivity reverse transcription system, ma tempulo a pg-level amathanso kupeza cDNA yapamwamba kwambiri.
- Makina osindikizira osinthika amakhala ndi kukhazikika kwamafuta ambiri, kutentha kwadongosolo kumatha kufika mpaka 50 ℃, ndipo imakhala ndi ntchito yabwino yolembera.
- Ngakhale zitsanzo zonyansa za RNA (mowa mpaka 60%, mchere wa guanidine mpaka 750mM) ukhoza kusinthidwanso.
- 2 × RT OR-YosavutaTMMix yawonjezera zoyambira zosinthira (Random Primer, Oligo(dT)18Choyamba).
Kit application
- Amagwiritsidwa ntchito mwachindunji mu Real Time PCR kusanthula mochulukira ma jini
- Imatha kusanthula mwachangu komanso molondola tinthu tating'ono ta RNA monga ma virus a RNA
- Reverse transcript of RNA templates with high GC content or complex the secondary structure
Kuyenda kwa ntchito
Kusungirako ndi moyo wa alumali
1. Mayendedwe
Njira yonse yoyendetsera kutentha kwa ayezi, kuonetsetsa kuti zida zili m'malo a <4°C.
2. Zosungirako
RT EasyTMII imasungidwa pa -20 ° C.Sungani mankhwalawa mufiriji yokhazikika pa -20 ° C mutangolandira.Ngati zosungirako zili zoyenera, mankhwalawa sangawononge ntchito iliyonse pazaka zovomerezeka za 1.
