◮Chida cha sitepe imodzi chimathandizira kuti zolembera zosinthika ndi PCR zizichitika mu chubu chomwecho.Zimangofunika kuwonjezera template ya RNA, zoyambira zenizeni za PCR ndi RNase-Free ddH2O.
◮Kusanthula kwanthawi yeniyeni kwa RNA kumatha kuchitika mwachangu komanso molondola.
◮Zidazi zimagwiritsa ntchito makina apadera a Foregene reverse transcript reagent ndi Foregene HotStar Taq DNA Polymerase ophatikizidwa ndi njira yapadera yamachitidwe kuti apititse patsogolo kukulitsa bwino komanso momwe zimachitikira.
◮Dongosolo lokonzekera bwino lomwe limapangitsa kuti zomwe zimachitikazo zikhale ndi chidwi chodziwikiratu, kukhazikika kwamafuta, komanso kulolerana bwino.