RT-qPCR Easyᵀᴹ (Chigawo Chimodzi) -Taqman
Kufotokozera Kwa Zida:
-Mmodzi-sitepe kit imathandizira kulembedwa kwa reverse ndi PCR kuti ichitike mu chubu chomwecho, muyenera kuwonjezera template RNA, zoyambira za PCR ndi RNase-Free ddH.2O.
- Kusanthula kwanthawi yeniyeni kwa ma virus a RNA kapena kufufuza RNA kumatha kuchitika mwachangu komanso molondola.
- Zidazi zimagwiritsa ntchito makina apadera a Foregene reverse transcription reagent ndi Foregene HotStar Taq DNA Polymerase ophatikizidwa ndi njira yapadera yamachitidwe kuti apititse patsogolo kukulitsa bwino komanso momwe zimachitikira.
- Dongosolo lokonzekera bwino lomwe limapangitsa kuti zomwe zimachitikazo zikhale ndi chidwi chodziwikiratu, kukhazikika kwamafuta, komanso kulolerana bwino.
- RT-qPCR YosavutaTM(One Step) -Taqman kit imabwera ndi utoto wamkati wa ROX, womwe ungagwiritsidwe ntchito kuthetsa zolakwika zamtundu wazizindikiro pakati pa zitsime, zomwe ndizosavuta kuti ogwiritsa ntchito azigwiritsa ntchito mitundu yosiyanasiyana ya zida za PCR.
Kufotokozera
Foregene One-Step RT-PCR Easy Series Zogulitsa zimazindikira momwe ma RNA amagwirira ntchito kupita ku DNA yazingwe ziwiri, ndiye kuti, kumasulira mobwerezabwereza ndi kukulitsa kwa PCR kumamalizidwa munjira yofananira ya centrifuge chubu ndi machitidwe omwewo, motero masitepe oyesera amakhala osavuta, pulani yoyesera imakongoletsedwa, komanso kuyesa bwino.
RT-qPCR YosavutaTM (MmodziGawo)-Taqmanpogwiritsa ntchito Foregene HotStar Taq DNA Polymerase, makina okhathamiritsa a Taqman qPCR amatha kuchita mwachangu komanso mwachindunji RT-qPCR kudziwa kuchuluka kwa ma tempuleti a RNA.
Zofotokozera
100 Rxns (20μl dongosolo)
500 Rxns (20μl dongosolo)
Zida zamagulu
| RT-qPCR EasyTM (Chigawo Chimodzi) -Taqman | ||
| Zida zamagulu | Mtengo wa RT-02131 | Mtengo wa RT-02132 |
| 100Rxns (20μl dongosolo) | 500 Rxns (20μl dongosolo) | |
| 2 × RT-qPCR YosavutaTMMix-Taqman | 1 ml | 1.7ml × 3 |
| 20 × ROX Reference Dye | 100μl | 0.5ml pa |
| RNase-Free ddH2O | 1.7ml pa | 1.7ml × 3 |
| Malangizo Ogwiritsa Ntchito | 1 | 1 |
Mbali & ubwino
- Chida cha sitepe imodzi chimathandizira kulembedwa kwa reverse ndi PCR kuchitidwa mu chubu chomwecho, kumangofunika kuwonjezera template RNA, zoyambira za PCR ndi RNase-Free ddH2O.
- Kusanthula kwanthawi yeniyeni kwa ma virus a RNA kapena kufufuza RNA kumatha kuchitika mwachangu komanso molondola.
- Zidazi zimagwiritsa ntchito makina apadera a Foregene reverse transcription reagent ndi Foregene HotStar Taq DNA Polymerase ophatikizidwa ndi njira yapadera yamachitidwe kuti apititse patsogolo kukulitsa bwino komanso momwe zimachitikira.
- Dongosolo lokonzekera bwino lomwe limapangitsa kuti zomwe zimachitikazo zikhale ndi chidwi chodziwikiratu, kukhazikika kwamafuta, komanso kulolerana bwino.
- RT-qPCR YosavutaTM(One Step) -Taqman kit imabwera ndi utoto wamkati wa ROX, womwe ungagwiritsidwe ntchito kuthetsa zolakwika zamtundu wazizindikiro pakati pa zitsime, zomwe ndizosavuta kuti ogwiritsa ntchito azigwiritsa ntchito mitundu yosiyanasiyana ya zida za PCR.
Zosintha za Kit
Kit application
- Zidazi zimagwiritsidwa ntchito pozindikira mwachangu komanso molondola ma RNAs monga ma virus a RNA.
- Ndizoyenera kuzindikira mwachangu ma tempulo a RNA okhala ndi GC yayikulu kapena mawonekedwe achiwiri ovuta.
Kayendedwe kantchito
Zosungirako
1.Mayendedwemikhalidwe
Njira yonse yamayendedwe otsika otsika oundana, kuonetsetsa kuti zida zili m'malo a <4 ℃.
2.Kusungirako mikhalidwe
Zida zimasungidwa pa -20 ℃.Kusunga mankhwala mu zonse kutentha firiji pa-20 ℃ atangolandira.Ngati zinthu zosungirako zili zoyenera, mankhwalawasichidzanyozetsa ntchito iliyonse pazaka zovomerezeka za 1.
