SARS-CoV-2 Variant Nucleic Acid Detection Kit II (Multiplex PCR Fluorescent Probe Method) -pozindikira mitundu yosiyanasiyana kuchokera ku UK, South Africa, Brazil, ndi India
Kufotokozera Kwa Zida:
◮ Direct PCR:Palibe chifukwa cha zida za nucleic acid extaction ndi System, zomaliza 96 mu ola limodzi kapena apo.
◮ Kufunika kwa zida zochepa komanso kugwiritsa ntchito kosavuta:Kungofunika Real-Time PCR system .
◮ LoD komanso kukhudzika kwakukulu:Kuzindikira kumatha kufika pamlingo wotsika kwambiri ngati makope 500/ml.
Zofotokozera
48 Rxns/kit, 96 Rxns/kit
| Ayi. | Chigawo | Ndalama | Chofunikira chachikulu | |||
| 48 nsi | 96 nsi | |||||
| 1 | Nucleic acid kumasula wothandizira | 1.4 mL / chubu | 2 machubu | 5.3 mL / botolo | 1 botolo | Wokwera pamwamba |
| 2 | Chitetezo cha RNA | 53 μL / chubu | 1 chubu | 106 μL / chubu | 1 chubu | RNase inhibitor |
| 3 | Mtundu wa SARS-CoV-2 reaction solution A | 800 μL / chubu | 1 chubu | 1600 μL / chubu | 1 chubu | Poyamba, probe, reaction buffer, dNTP |
| 4 | Mtundu wa SARS-CoV-2 reaction solution B | 800 μL / chubu | 1 chubu | 1600 μL / chubu | 1 chubu | Poyamba, probe, reaction buffer, dNTP |
| 5 | SARS-CoV-2 enzyme osakaniza | 160 μL / chubu | 1 chubu | 320 μL / chubu | 1 chubu | Kutentha koyambira kwa Taq enzyme, enzyme ya M-MLV |
| 6 | SARS-CoV-2 kulamulira kwabwino | 100 μL / chubu | 1 chubu | 100 μL / chubu | 1 chubu | Recombinant plasmid yokhala ndi chidutswa chandamale, RNA |
| 7 | Kuwongolera koyipa kwa SARS-CoV-2 | 1200 μL / chubu | 1 chubu | 1200 μL / chubu | 1 chubu | TE buffer |
Chidziwitso: Zida zamitundu yosiyanasiyana sizingagwiritsidwe ntchito mosinthana.
Zina & Ubwino:
Mwachangu komanso mophweka: malizitsani mayeso pafupifupi ola limodzi
Pewani kutaya kwa nucleic acid: palibe chifukwa choyeretsera nucleic acid
Malire odziwikiratu otsika komanso kukhudzika kwakukulu, makamaka suitabe kuti azindikire mwachangu kwambiri
Kufunika kwa zida zochepa komanso kugwiritsa ntchito kosavuta
Kuzindikira kumatha kufika pamlingo wotsika kwambiri ngati makope 500/mL).
Momwe mungagwiritsire ntchito:
1.Chida chothandizira
ABI7500,Bio-Rad CFX96,Roche LightCycler 480,SLAN-96S
2.Njira yodziwira
Kukonzekera kwachitsanzo: Naopharyngeal kapena oropharyngeal swab chitsanzo (opareshoni imodzi yokha) (Dikirani kwa mphindi 10)
PCR amplificaton (<= 55min)
Kayendedwe kantchito:
Posungira:
Osindikizidwa kuchokera ku kuwala ndi kusungidwa pa -20 ± 5 ℃;
Sungani pa 2-8 ℃ mutatsegula, gwiritsani ntchito mkati mwa masiku 7;pewani kuzizira mobwerezabwereza (<5 nthawi)
Alumali moyo:1 chaka
