Dothi DNA Isolation Kit Extraction Purification Kits & Reagents for Dothi DNA
Kufotokozera Kwa Zida:
Yeretsani mwachangu ndikupeza DNA yapamwamba kwambiri kuchokera ku tizirombo tating'ono tadothi.
◮Palibe kuipitsidwa kwa RNase:DNA-Only Column yoperekedwa ndi zidayi imapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda kuwonjezera RNase panthawi yoyesera, kuletsa labotale kuti isaipitsidwe ndi RNase yachilendo.
◮Liwiro:Foregene Protease imakhala ndi zochita zambiri kuposa ma protease ofanana, ndipo imagaya zitsanzo za minofu mwachangu;ntchitoyo ndi yosavuta, ndipo ntchito yochotsa DNA ya genomic imatha kutha mkati mwa mphindi 20-80.
◮Zabwino:The centrifugation ikuchitika kutentha firiji, ndipo palibe chifukwa 4 ° C otsika kutentha centrifugation kapena Mowa mpweya wa DNA.
◮Chitetezo:Palibe organic reagent m'zigawo zofunika.
◮Mapangidwe apamwamba:DNA yotulutsidwa ya genomic ili ndi zidutswa zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira zosiyanasiyana.
◮Microelution System:Itha kuwonjezera kuchuluka kwa ma DNA amtundu, omwe ndi osavuta kuzindikira kapena kuyesa kuyesa.
Kufotokozera
Chidachi chimapereka njira yachangu komanso yosavuta yochotsera DNA ya ma genomic kuchokera kudothi zosiyanasiyana.Pali zoletsa zambiri monga humic acid ndi ayoni achitsulo mu zitsanzo za nthaka.Ngakhale zinthuzi zitakhalapo pang'ono mu DNA yoyeretsedwa, zimakhala ndi mphamvu pazochitika zapansi, monga PCR ndi kuletsa kugaya kwa enzyme.Choncho, chinsinsi kuyeretsa nthaka DNA ndi mmene bwino kuchotsa inhibitors m'nthaka.
Chidachi chimagwiritsa ntchito DNA-Only Column yomwe ingathe kumangirira ku DNA, Foregene Protease yatsopano komanso njira yapadera yosungiramo chitetezo, yomwe imatha kuchotsa zoletsa zosiyanasiyana m'nthaka popanda kutulutsa zosungunulira za organic kapena ethanol ndi mpweya wa isopropanol.Kutulutsa kwa DNA kuchokera ku dothi kumatha kutha mphindi 90.
Zofotokozera
50 Preps, 100 Preps, 250 Preps
Zida zamagulu
| Mtengo wa SG1 |
| Mtengo wa SG2 |
| Mtengo wa SG3 |
| Mtengo wa SG4 |
| Mtengo PW |
| Mtengo WB |
| Mtengo wa EB |
| Mtengo wa TE |
| Foregene Protease |
| Nambala ya DNA Yokha |
| Malangizo |
Mbali & ubwino
-Palibe kuipitsidwa kwa RNase: Gawo la DNA-Only loperekedwa ndi zidali limapangitsa kuti zitheke kuchotsa RNA ku genomic DNA popanda kuwonjezera RNase panthawi yoyesera, kupewa labotale kuti isaipitsidwe ndi RNase yachilendo.
-Kuthamanga Kwambiri: Ntchitoyi ndi yosavuta, ndipo ntchito yochotsa DNA ya nthaka imatha kutha mkati mwa mphindi 90.
- Yabwino: The centrifugation ikuchitika kutentha firiji, ndipo palibe chifukwa 4°C kutentha pang'ono centrifugation kapena mpweya wa ethanol wa DNA.
-Safety: Palibe organic reagent m'zigawo zofunika.
-Zapamwamba kwambiri: DNA yotulutsidwa ya genomic ili ndi zidutswa zazikulu, palibe RNA, palibe RNase, komanso ma ion otsika kwambiri, omwe amatha kukwaniritsa zofunikira za mayesero osiyanasiyana.
Kit application
Ndiwoyenera kuyeretsedwa kwa DNA ya genomic kuchokera ku zitsanzo zotsatirazi: zitsanzo za silt, dothi la maluwa, nthaka yopondapo, nthaka ya m'nkhalango, nthaka yamtunda, nthaka yamadzi, nthaka ya m'mphepete mwa nyanja, nthaka ya udzu ndi zitsanzo zina.
Kayendedwe kantchito
Kusungirako ndi moyo wa alumali
-Chida ichi chikhoza kusungidwa kwa miyezi 12 pansi pa nyengo youma kutentha (15-25 ° C), ngati chiyenera kusungidwa kwa nthawi yaitali, chikhoza kusungidwa pa 2-8 ° C.
Zindikirani: Ngati atasungidwa kutentha kochepa, yankho limakhala lamvula.Musanagwiritse ntchito, onetsetsani kuti mwayika yankholo mu zida pa firiji kwakanthawi.Ngati n'koyenera, preheat mu madzi osamba 37 ° C kwa mphindi 10 kuti asungunuke mpweya, ndikusakaniza musanagwiritse ntchito.
-Foregene Protease yankho lili ndi ndondomeko yapadera, yomwe imagwira ntchito ikasungidwa kutentha kwa nthawi yaitali (miyezi itatu);ntchito yake ndi kukhazikika kwake kudzakhala bwino pamene kusungidwa pa 4 ° C, choncho tikulimbikitsidwa kusunga pa 4 ° C, kumbukirani kuti musamasunge -20 ° C.
-Dry Lysozyme ufa amasungidwa pa -20 ° C;njira yothetsera Lysozyme imagawidwa m'magawo ang'onoang'ono ndikusungidwa pa -20 ° C.
